Illuminate hepatobiliary and pancreatic surgery using optical molecular imaging methods / 中华肝胆外科杂志

Since the concept of molecular imaging was put forward in 1999,optical molecular imaging techniques have been widely applied in the field of biomedical and clinical research.Its unique application value is especially shown in hepatobiliary surgery,such as in liver tumor imaging,anatomical liver resection,liver transplant angiography,cholangiography,and bile or pancreatic leakage prevention.Optical molecular imaging technique "lights up" targeted areas in surgical operations and provides convenience in carrying out precision operation.This paper reviewed the advantages of optical molecular imaging technology in clinical research and discussed its limitations in translational surgery,and put forward possible directions in improvement for the future.

Preclinical study of bladder cancer diagnosis using tumor homing peptide fluorescent molecular probe / 中华泌尿外科杂志

Objective To evaluate the features of optical molecular imaging of bladder tumor cells labeled by tumor homing peptide fluorescent molecular probe, and to explore the theoretical foundation of optical molecular imaging for bladder cancer diagnosis.Methods After prepared the FITC-CSNRDARRC fluorescent molecular probe, laser scanning confocal microscope, immuno fluorescence and multispectral fluorescence in vivo optical molecular imaging system have been used to evaluate the binding sites, the affecting factors of binding rates, the specificity and the targets.BIU-87 bladder tumor cell line, BIU-87 bladder tumor cell line, 68 cases of paraffin bladder tumor tissue samples, 16 cases of paraffin glandular bladder inflammatory samples, 43 cases of paraffin renal clear cell carcinoma samples, 68 cases of paraffin gastric adenocarcinoma samples, 29 cases of urine exfoliated cells suspected bladder cancer and BIU-87 bladder cancer nude xenograft have been used in this study.Results The binding site of FITC-CSNRDARRC fluorescent molecular probe were at the nucleus of labeled bladder tumor cells.The binding rates were correlated linearly with the dose of probe and the grade of pathology.The in vitro and in vivo studies demonstrate that the probe has a binding specificity with bladder tumor. When the FITC-CSNRDARRC fluorescent molecular probe labeled tumor cells, bright green spots were observed under laser scanning confocal microscope.The bright green spots were more apparent after stained by DAPI again.The tissue samples and tumor cells in the urine can be successful labeled and identified by fluorescence microscope.Optical molecular imaging of in vivo xenograft tumor tissues showed fluorescent spots under EMCCD.Conclusions The labeled loci of single cell by FITC-CSNRDARRC probe have been identified. The spatial resolution of optical molecular image is related to sensitivity of CCD, and the optical molecular imaging cannot be imaged by the conventional endoscope camera.

Effects of Testosterone on the Intracavernosally Injected Embryonic Stem Cells in Rat / 대한남성과학회지

PURPOSE: Stem cell-based cell therapy has recently been tried as a way to restore cavernosal function in an animal model. The aim of this study was to elucidate the role of testosterone on intracavernosally injected embryonic stem cells (ESCs) in rat. MATERIALS AND METHODS: Male Sprague Dawley rats(12 weeks old; n=25) were divided into five groups: control, castration, ESC injection after castration, testosterone supplementation after castration, and ESC injection with testosterone supplementation after castration(n=5 in each group). ESCs were transfected with firefly luciferase attached to adenovirus and then injected intracavernously. Testosterone propionate(0.1 mg) was subcutaneously injected daily in the testosterone supplementation group. Cell survival was assessed by optical molecular imaging the day after the ESC injection. After 4 weeks of treatment, intracavernosal pressure and systemic arterial pressure were recorded after pelvic nerve stimulation. Serum testosterone levels were measured by radioimmunoassay. RESULTS: With optical molecular imaging, we observed fluorescent signals around the external genitalia of all ESC-injected animals. The percentage of intracavernosal pressure/systolic blood pressure was significantly lower in the castration group(15.9+/-5.3%) compared to the control group(53.7+/-9.7%)(p0.05). The ESC injection with testosterone supplementation group(54.2+/-4.3%) showed erectile function similar to the control group. CONCLUSIONS: Intracavernosal injection of embryonic stem cells did not improve erectile function in the castrated rat. This result implies that testosterone may play an essential role in the proliferation of stem cells in the corpus cavernosum.

Effects of Testosterone on the Intracavernosally Injected Embryonic Stem Cells in Rat / 대한남성과학회지

PURPOSE: Stem cell-based cell therapy has recently been tried as a way to restore cavernosal function in an animal model. The aim of this study was to elucidate the role of testosterone on intracavernosally injected embryonic stem cells (ESCs) in rat. MATERIALS AND METHODS: Male Sprague Dawley rats(12 weeks old; n=25) were divided into five groups: control, castration, ESC injection after castration, testosterone supplementation after castration, and ESC injection with testosterone supplementation after castration(n=5 in each group). ESCs were transfected with firefly luciferase attached to adenovirus and then injected intracavernously. Testosterone propionate(0.1 mg) was subcutaneously injected daily in the testosterone supplementation group. Cell survival was assessed by optical molecular imaging the day after the ESC injection. After 4 weeks of treatment, intracavernosal pressure and systemic arterial pressure were recorded after pelvic nerve stimulation. Serum testosterone levels were measured by radioimmunoassay. RESULTS: With optical molecular imaging, we observed fluorescent signals around the external genitalia of all ESC-injected animals. The percentage of intracavernosal pressure/systolic blood pressure was significantly lower in the castration group(15.9+/-5.3%) compared to the control group(53.7+/-9.7%)(p0.05). The ESC injection with testosterone supplementation group(54.2+/-4.3%) showed erectile function similar to the control group. CONCLUSIONS: Intracavernosal injection of embryonic stem cells did not improve erectile function in the castrated rat. This result implies that testosterone may play an essential role in the proliferation of stem cells in the corpus cavernosum.