ABSTRACT
Abstract Citrus fruit production occupies a place of considerable importance in the economy of the world including Pakistan. Tristeza disease caused by Citrus Tristeza Virus (CTV) exists in various forms that may or may not cause symptoms in the plants. The bioactive compounds and antioxidants are naturally present in plants and provide a defense mechanism that is generally accelerated in response to a stress. The objective of the present study was to target and analyze the citrus plants that were CTV positive to observe the changes in the enzymatic and non-enzymatic antioxidants of citrus (Sweet Oranges only). It was observed that in response to CTV infection, both the non-enzymatic antioxidants (total flavonoid, ascorbic acid, phenolic acid) and enzymatic antioxidants (catalase, superoxide dismutase and peroxidase) activities showed an increasing trend overall. The profiling of antioxidants in response to a viral infection may help in the discovery of new biomarkers that can be used as a monitoring tool in disease management.
Resumo As frutas cítricas ocupam um lugar de considerável importância na economia do Paquistão, assim como o resto do mundo. A doença da tristeza causada pelo Vírus da Tristeza dos Citros (CTV) existe em várias formas que podem ou não apresentar sintomas nas plantas. Os compostos bioativos e antioxidantes estão naturalmente presentes nas plantas e fornecem um mecanismo de defesa que é geralmente acelerado em resposta a um estresse. O objetivo do presente estudo foi analisar as alterações causadas pelo CTV nos antioxidantes enzimáticos e não enzimáticos de laranjas doces. Foi observado que, em resposta ao ataque de CTV, os antioxidantes não enzimáticos como flavonoides totais, ácido ascórbico, ácido fenólico e antioxidantes enzimáticos, como as atividades de catalase, superóxido dismutase e peroxidase, geralmente mostram uma tendência crescente. O perfil de antioxidantes em resposta a um ataque viral pode ajudar na descoberta de novos biomarcadores que podem ser usados como uma ferramenta de monitoramento no gerenciamento de doenças.
Subject(s)
Plant Diseases/prevention & control , Plant Diseases/virology , Closterovirus/physiology , Citrus sinensis/enzymology , Citrus sinensis/chemistry , Antioxidants/analysis , Antioxidants/classification , Ascorbic Acid/analysis , Flavonoids/analysis , Catalase/analysis , Peroxidase/analysisABSTRACT
Resumen Los estudios de la araneofauna en los agroecosistemas son cruciales para la implementación de las arañas como agentes potenciales de control biológico de plagas. En este trabajo se analiza la distribución del ensamble de arañas en los microhábitats (follaje, tronco y suelo) de los cultivos de Citrus sinensis, (Rutaceae) de la Provincia de Corrientes, Argentina. Los muestreos se realizaron mensualmente mediante cuatro técnicas de captura: agitación de follaje, tamizado de hojarasca, pitfall y captura manual. En total se obtuvieron 2 160 muestras y 7 194 ejemplares de arañas (2 462 en follaje, 983 en tallo y 3 749 en suelo) pertenecientes a 35 familias y 200 especies de Araneomorphae. Hubo diferencias significativas en la estructura de las comunidades de arañas de los tres microhábitats y entre los meses, según el análisis de similitud (ANOSIM). El follaje presentó mayor diversidad, seguido por el suelo y el tronco. Araneidae, Theridiidae y Salticidae predominaron en el follaje; las especies dominantes fueron: Jessica erythostoma y Cheiracantium inclusum, esta araña es una de las principales depredadoras de las larvas de Phyllocnistis citrella (Lepidoptera) que atacan las hojas de Citrus. En el tronco abundaron: Tetragnathidae, Hersiliidae y Theridiidae, destacándose Iviraiva pachyura, una especie especialista en dicho microhabitat; y Leucauge venusta la cual consideramos que es una especie oportunista, debido a que su abundancia se incrementa notablemente en sitios modificados por el hombre. En el suelo abundaron: Lycosidae, Linyphiidae y Coriniidae; Lycosa erythrognatha fue la especie dominante, seguida de Pardosa plumipedata. El índice de similitud Chao-Jaccard fue mayor entre el follaje y el tronco (J-C = 0.95), asimismo el ordenamiento Escalado Multidimencional no Métrico (NMDS) mostró una clara diferenciación de la composición de especies del suelo con respecto al follaje y al tronco. Además, en el suelo hubo mayor recambio temporal de especies y el método de seriación resultó significativo (z = 3.82, P = 0 .00) por lo tanto hubo una sucesión de especies durante el año de muestreo. Las especies en cada microhabitat probablemente evitan la competencia al utilizar distintas estrategias de caza y recursos ecológicos, además de patrones específicos de distribución temporal. Nuestros resultados sugieren que las modificaciones del ambiente, como en estos cultivos de C. sinensis, tienden a albergar varias poblaciones de arañas que se adaptaron a dicho cambio e interactúan de manera equilibrada en espacio y tiempo.(AU)
Abstract Arachnofauna studies from agroecosystems are crucial for implements spider as biological control agents. In this work we analyze the distribution of spider assemblages at three microhabitats (canopy, tree trunk and soil) in Citrus sinensis orchards (Rutaceae) in the Province of Corrientes, Argentina. Spiders were collected monthly using four sampling techniques: beating foliage, leaf litter sifting, pitfall traps and direct capture by hand. A total of 2 160 samples were examined and 7 194 spiders were collected (2 462 on the canopy, 983 on trunk and 3 749 on the floor) belonging to 34 families and 200 species/ morphospecies of Araneomorphae. There were significant differences among microhabitats and months in the structure of the spider communities, according to analysis of similarity (ANOSIM). The diversity was highest on the canopy, followed by soil and tree trunk. Araneidae, Salticidae and Anyphaenidae were the most abundant taxa in the canopy; the dominant species were Jessica erythostoma and Cheiracanthium inclusum, a predator of the leaf herbivore Phyllocnistis citrella (Lepidoptera) a pest of Citrus spp. The most abundant families on the tree trunk were Tetragnathidae, Hersiliidae and Theridiidae, Iviraiva pachyura was a species specialist in such microhabitat; and Leucauge venusta was an opportunistic species, because their abundance increaseds markedly in anthropic environments that modified by man. The most abundant families on the floor were Lycosidae, Linyphiidae and Coriniidae. In this habitat, Lycosa erythrognatha and Pardosa plumipedata were dominant species. The Chao-Jaccard similarity index was highest between the canopy and trunk (J-C = 0.95), likewise ordination by nonmetric multidimensional scaling (NMDS) revealed strong differentiation between assemblages of soil spiders and canopy and trunk spiders. Also, on the soil the temporary replacement of species was greater and the seriation method was significant (z = 3.82, P = 0.00) therefore there was a succession of species during the year. The different species in each microhabitat probably avoided competition by using different hunting strategies and different ecological resources, in addition to specific patterns of temporal distribution. Our results suggest that anthropic environments, such as in Citrus orchards, tend to harbor populations of spiders able to adapt to those environments and interact in a balanced way in space and time.(AU)