ABSTRACT
The constituents of the whole plant of Orobanche coerulescens were isolated and purified by using various column chromatographic techniques including D101, silica gel and ODS. The structures were identified by spectroscopic analyses including NMR and MS. A new phenylethanol glycoside was isolated from the whole plant of O. coerulescens, and was identified as 2-(3-methoxy-4-hydroxyphenyl)-ethanol-1-O- [(1→3)-O-α-L-rhamnopyranosyl-4, 6-O-di-feruloyl]-β-D-glucopyranoside, named as orobancheoside B. Through the antibacterial activity test, orobancheoside B was proved to have certain antibacterial activity, and be one of the main active components of O. coerulescens. The research result will laid a foundation for the medicinal materials and quality control research. Activity screening, broomrape orobancheoside B has certain antibacterial activity, as one of the main active components of O. coerulescens, and to constantly improve the quality of the medicinal materials laid a foundation.
ABSTRACT
Objective: To study the chemical constituents from the whole plant of Orobanche coerulescens. Methods: The constituents were isolated and purified by using various column chromatographic techniques including D101, silica gel, and ODS, and the structures were identified by spectroscopic analyses including HR-ESI-MS, NMR, and 2D-NMR. Results: A new phenethyl alcohol glycoside was isolated from 50% ethanol extract in the whole plant of O. coerulescens, and its structure was identified as 2-(3-methoxy-4-hydroxy)-phenyl-ethanol-1-O-α-L-[(1→3)-4-O-acetyl-rhamnopyranosyl-4-O-feruloyl]-O-β-D-glucopyranoside. Conclusion: Compound 1 is a new compound named as orobancheoside A.