The effect of the new hemostatic agent Ostene on bone healing: an experimental study in rabbits

Introduction: Ostene is a water-soluble wax-like alkylene oxide copolymer preparation for use as a mechanical hemostatic agent. this study aims to evaluate the effects of Ostene on bone healing. materials and methods: twenty albino rabbits were divided into four groups according to post-treatment follow-up (24 hr, 3 days, 7 days, 14 days) with five rabbits in each group. each rabbit in all groups was treated with two study materials (Ostene and Gelfoam). three holes were made in the mandibular bone of each rabbit using 5mm surgical bur; two holes were made on right side: one for testing Ostene and another for Gelfoam. a third hole, on the left side of mandible, was not treated, and was used as a control. finally, the incision was closed. the specimens were collected at different days post-treatment and examined by histopathology. result and discussion: this study showed that there is a significant difference (p-value≤ 0.05) between the Ostene group and the other groups (Gelfoam and control). at 24 hr post intervention, there is a significant difference in osteoblast cell formation (p-value=0.03), and osteoclast cell formation (p-value=0.05). new blood vessel formation, osteoblast and osteoclast cell formation for Ostene group at 3 days post-intervention were also significantly different (p-values = 0.05, 0.03, 0.04, respectively). at 7 days post-intervention p-values were 0.05 for osteoblast formation and 0.04 for osteoclast formation, respectively. after 14 days of healing p-value for osteoblast cell formation in the Ostene group was 0.05 and 0.04 for osteoclast cell formation. conclusions: the bone hemostatic agent Ostene is an effective at enhancing osteogenesis by initiating proliferation of osteoblast and osteoclast cells.

Comparison of ostene and bone wax on bone healing: a comparative experimental study in rabbits

Introduction: Ostene is a new synthetic bone hemostatic wax-like inert and biocompatible material that dissolves within two days after application. bone wax is a well- known topical hemostatic agent, easy to use, and its application is very simple. wound healing is a complex biological process; bone is a dynamic tissue that is continuously resorbed, renewed, and remodeled. materials and methods: twenty domestic rabbits were divided into four groups (day 1, day 3, day 7, and day 14). each rabbit was anaesthetized and three holes were drilled in the mandible: one was filled with Ostene, another with bone wax, and the other was left unfilled as control. sites of intervention were assessed by histopathology. results and Discussion: Ostene and bone wax showed osteoinductive property in bone healing with no inflammatory reaction. our study revealed new bone formation within 14 days in Ostene group. after histopathological analysis and scoring was finished, analysis by SPSS 14 software showed a significant difference between the use of Ostene and bone wax. conclusion: Ostene showed superiority over bone wax in bone healing, and it can be used in the same way as bone wax with no interference with bone healing and osteogenesis. Ostene has no side effects following application.