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OBJECTIVE: To prepare osteogenic growth peptide(OGP) nasal spray and investigate the absorption characteristics. METHODS: The absorption enhancer was selected by orthogonal experiment, using the declining quantity of osteogenic growth peptide in rat nose as evalution index with rat nasal perfusion model, and further the dose of protease inhibitor was selected. Finally, the acceletated and long term test were made to investigate the stability of osteogenic growth peptide nasal spray according to Chinese Pharmacopoeia 2010. The rat nasal mucosal shape were inspected by electron microscope after perfusion of osteogenic growth peptide nasal spray. RESULTS: The optimal prescription of osteogenic growth peptide nasal spray is composed of osteogenic growth peptide 2 mg, citric acid 50 mg, HP-β-CD 100 mg, Tween-80 300 mg, and bacitracin zinc 50 mg in 10 mL patossium hydrogen phthalate buffer (pH 3.0). Osteogenic growth peptide nasal spray is much lower irritation to the nasal mucosa cilium. CONCLUSION: The stablity of osteogenic growth peptide nasal spray was good, safe and had very good absorption in rat nose.
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Implants have been widely used in restorative treatment for patients who have undergone head and neck cancer surgery. With the development of combination treatment of head and neck cancer, radiotherapy has been a common means of therapy. However, it could induce various changes in hard and soft tissues and reduce the success and survival rate of the implants. Some research, using either animal models or clinical studies, have shown that certain strategies could be used for improving the survival rate of implants. In this review, we discussed the changes in both hard and soft tissues, which may reduce the survival rate of the implants, and the proposed methods for improving the survival rate of patients after radiotherapy.
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Humans , Bone Morphogenetic Proteins , Dental Implants , Head and Neck Neoplasms , Head , Hyperbaric Oxygenation , Methods , Models, Animal , Osteoradionecrosis , Radiotherapy , Survival RateABSTRACT
Objective To investigate the effects of osteogenic growth peptide (OGP) in combination with extracorporeal shock waves (ESWs) on osteoblast proliferation.Methods Passaged cells were divided into four groups for different treatments:a control group,an OGP + ESW group,an ESW group,and an OGP group.After the respective treatments,the cells were cultured for 24 h,48 h and 72 h and counted using methylthiazdy tetrazolium (MTT) and an inverted fluorescence microscope. Immunohistochemical examination was used for detecting protein kinase A (PKA)activity,and a reverse transcription-polymerase chair reaction (RT-PCR) was used for examining PKA mRNA expression at 24 and 48 hours.Results Cell counting revealed that cell proliferation in the OGP + ESW,ESW and OGP groups was significantly promoted compared with the control group.Cell proliferation was greatest in the OGP + ESW group.The immunohistochemical examination showed positive staining intensities in the OGP + ESW,ESW and OGP groups significantly higher than in the control group.The positive staining intensity in the OGP + ESW group was again the highest.PKA activity was also significantly higher in the OGP + ESW,ESW and OGP groups than in the control group with the level in the OGP + ESW group the highest.Conclusion OGP in combination with ESW has a synergistic effect in stimulating osteoblast proliferation and growth.
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Objective:To develop an enzyme immunoassay for the detection of Osteogenic Growth Peptide in serum for clinical investigation.Methods:Two kinds of competitive Enzyme linked immununosorbent assay were employed,based on sensitivity and shape of dose-reaction curve,the most suitable testing pattern was selected for further application.Results:Purified antibody was better than salting-out antibody in competitive Enzyme linked immununosorbent assay in terms of standard curve.Modified competitive Enzyme linked immununosorbent assay was better than competitive Enzyme linked immununosorbent assay in sensitivity.Conclusion:Modified competitive Enzyme linked immununosorbent assay for Osteogenic Growth Peptide is sensitive enough for clinical investigation.
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Objective:To elucidate the effect of osteogenic growth peptide (OGP) on proliferation and differentiation of human periodontal ligament cells on titanium disc in vitro. Methods:Human periodontal ligament cells were cultured located on titanium disc individually in medium contained various concentrations of OGP;and cell proliferation quantity were examined by MTT and alkaline phosphatase activity were examined by enzyme-linked immunosorbentassay. Results:In vitro,the addition of OGP in cultures resulted in an increase of cell proliferation and ALP activity(P
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Objective:To elucidate the effect of osteogenic growth peptide(OGP) on proliferation and protein synthesis of human PDLCs in vitro.Methods:Human PDLCs were cultured individually in medium contained various concentrations of OGP;and cell proliferation quantity and protein synthesis were examined by MTT and coom asie brilliant blue staining.Results:In vitro,the addition of OGP in cultures resulted in an increase of cell proliferation(P