ABSTRACT
Objective:To study MTDH and PDCD1O level in colorectal cancer and its clinical significance.Methods:Between December 2014 and December 2015,55 cases of colorectal cancer and 55 intestinal polyps were selected as the research object.Real-time fluorescence quantitative technology(RT-PCR) was used to detect MTDH and PDCD10.Results:By RT-PCR detection,Colorectal cancer tissue MTDH,PDCD10 mRNA was higher than tissue adjacent to carcinoma,colon polyps group,and the difference was statistically significant (P＜0.05),and MTDH,PDCD10 mRNA level comparison of tissue adjacent to carcinoma,intestinal polyp tissue,there was no statistically significant difference (P＞0.05).MTDH,PDCD10 mRNA level of colorectal cancer tissues showed no significant difference between the patients with different age,gender (P＞0.05),and with the increase of patients with malignant degree,MTDH,PDCD10 mRNA level was rising,MTDH,PDCD10 expression in colorectal cancer tissues was associated with liver metastasis,TNM staging,lymph node metastasis.MTDH,PDCD10 expression in the organization of Ⅲ-Ⅳ,with liver metastasis and lymph node metastasis of colorectal cancer patients,was higher than Ⅰ-Ⅱ,no liver metastasis and lymph node metastasis of colorectal cancer patients (P＜0.05).MTDH expression in colorectal cancer tissues positively correlated with the expression of PDCD10 (r=0.409,P=0.409).Conclusions:Expressions of MTDH,PDCD10 in colorectal cancer tissues were higher than normal tissue and intestinal polyp tissues.MTDH,PDCD10 of colorectal cancer tissues associated with TNM stages,differentiation degree,distant metastasis,liver metastasis.
ABSTRACT
Homo sapiens PDCD10(programmed cell death 10,alias,"TF-1 cell apoptosis related gene 15,TFAR15"),cloned by means of cDNA-representational differences analysis,had been initially identified associated with cell apoptosis.Recent research suggested mutations within the PDCD10 gene or deletion were responsible for cerebral cavernous malformations,and PDCD10 was the third CCM gene.On the other hand,other research demonstrated that PDCD10 was strictly modulated and up regulated in many kinds of tumors,which implicated that PDCD10 participated in tumorous signal transduction.The recent research confirmed that PDCD10 interacts with MST4,a member of Ste20-related kinases,and the interaction promoted cell proliferation and transformation via modulation of the ERK-MARK pathway.In conclusion,all these demonstrate that PDCD10 has many biological effects,which suggests that it is a novel player in vascular morphogenesis and/or remodeling,as well as tumorigenesis and cancer progression.