Effect of Rhizobium, PSB and Mo on the Growth of Different Varieties of Kabuli Chickpea (Cicer kabulinum L.)

The present investigation was conducted with the objective of finding out the Effect of Rhizobium, PSB and Mo on the Growth of Different Varieties of Kabuli Chickpea. The study comprised six treatments of Inoculants I1-Control, I2-Rhizobium(Rh.)+phosphate solubilizing bacteria (PSB) seed inoculation, I3-Molybdenum (Mo)@0.5 g AM* kg-1 seed, I4-Molybdenum @1.0 g AM kg-1 seed, I5-Rh.+PSB+Mo seed treatment @0.5g AM kg-1 seed, I6-Rh.+PSB+Mo seed treatment 1.0g AM kg-1 seed and two varieties V1-RVSJKG 102, V2-Phule G 0517, evaluated in Factorial randomized block design (FRBD) with three replications. The results of the present study revealed that the seed inoculation, I6Rh.+ PSB + Mo@1 g AM kg-1 seed was found to be the best among other inoculants with respect to growth in chickpeas, and Variety Phule G 0517 produced higher values of growth and yield attributing.

Effect of Plant Growth Regulators and Biofertilizer on the Yield of Fenugreek (Trigonella foenum-graecum L.)

The present investigation entitled “Effect of plant growth regulators and bio fertilizer on growth and quality of fenugreek (Trigonella foenum-graecum L.)” was carried out at the Experimental field, College of Agriculture, RVSKVV, Gwalior during 2020-21 under agro-climatic and soil conditions of Northern Madhya Pradesh. The experiment was laid out in Randomized Block Design (RBD) with three replications. Each replication consists of ten treatments of biofertilizers (viz., Rhizobium, PSB and KSB) and plant growth regulators (viz., GA3 and NAA). All the treatments were randomized separately in each replication. Result showed that the different biofertilizers (i.e. Rhizobium, PSB and KSB) and plant growth regulators (i.e. GA3 and NAA) treatments significantly influenced the yield, yield parameters and economics parameters of fenugreek. Treatment T4 (Rhizobium + NAA) gave the maximum yield, yield parameter and economic parameter it was found best treatment combination as compared to all other treatment combinations of biofertilizers and PGRs, while the minimum yield, yield parameters and economics parameters were recorded in treatment T1 (Control).

Effect of Spacing and Biofertilizers on Growth and Yield of Chickpea

The field experiment was conducted during rabi 2022-23, at Crop Research Farm, Department of Agronomy, SHUATS, Prayagraj (U.P). The soil of the experimental plot was sandy loam in texture, nearly neutral in soil reaction (pH 6.9), low in organic carbon (0.32%), available N (283.93 kg/ha), available P (18.3 kg/ha) and available K (223.5 kg/ha). The experiment was laid out in Randomized Block Design with ten treatments viz. T1: Spacing 20 cm x 15 cm + Uninoculated, T2: Spacing 20 cm x 15 cm + Rhizobium, T3: Spacing 20 cm x 15 cm + PSB, T4: Spacing 30 cm x 10 cm + Uninoculated, T5: Spacing 30 cm x 10 cm + Rhizobium, T6: Spacing 30 cmx 10 cm + PSB, T7: Spacing 40 cm x 10 cm + Uninoculated, T8: Spacing 40 cm x 10 cm + Rhizobium, T9: Spacing 40 cm x 10 cm + PSB and T10: Control: 100% RDF each replicated thrice. The results of the experiment obtained that application of Rhizobium (20 g/kg seeds) along with the Spacing 30cmx10cm significantly increased the growth parameters viz. plant height (46.79 cm), number of nodules (31.93/plant), plant dry weight (22.11 g/plant) and yield parameters viz. number of pods per plant (36.80), seed index (22.15 g), seed yield (3.44 t/ha) and stover yield (4.28 t/ha). This treatment also showed its positive effect on economics viz. gross returns (1,71,834 INR/ha), net returns (1,24,486 INR/ha), B:C (2.63). As the majority of Indian farmers are marginal and poor who cannot afford the use of high cost fertilizers with the result that crops are exposed to nutritional constraints. In such conditions biofertilizers can play an important role in contributing towards soil health and crop productivity. Biofertilizers are a cost effective renewable energy source that plays a crucial role in reducing inorganic fertilizer application and at the same increasing crop yield besides maintaining soil fertility. The optimum spacing for any crop means maintaining uniform and healthy crop stand so as to get optimum yield of crop. The growth and yield of crop is closely related to number of plants per unit area.

Response of China aster to Bio-inoculant Amendments under Pot Experiment

The present investigation was layout out in Completely Randomized design (CRD) to assess the impact of biofertilizers on China aster with total of nine treatments and each treatment replicated thrice. The treatments consist of different combinations of bio-inoculants (Azospirillum, PSB, VAM and KSB). The results revealed that the treatment T8 (75%RDF+Azospirillum+PSB) was found significantly higher compared to other treatment combination, which recorded highest plant height (27.28 cm), Number of leaves (27.44), plant spread (15.28 cm2), Days to bud emergence (47.11 days), days of first bud break (54.67 days), opening first flower (62.33 days),number of flowers per plant(14.12), stalk length (13.00 cm), flower dimeter (4.92cm),Vase life (12.22 days), Leaf area (14.11 cm2).The economics viz. Gross return (Rs. 16,800), Net return (Rs. 8928) and Benefit cost ratio (2.13) was found highest in the same treatment.

Substitution of Nitrogen and Phosphorous by Bio-Inoculants and Their Effect on Growth Parameters, Yield Attributes and Yield of Barley

A field experiment was conducted during Rabi season of 2022-2023 at Rajoula Agriculture farm, of Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya Chitrakoot, Satna (M.P.). The present experiment having 13 treatment combinations replicated thrice in randomized block design. Barley variety HUV-113 was grown with recommended agronomic practices. On the basis of the results emanated from present investigation, it could be concluded that application of T11 [100 % N & P + PSB + Azotobacter + 100 % K] significantly recorded maximum growth parameters of barley such as plant population plot-1 (41.56 m-2), plant height (91.78 cm), and number of tillers (87.74) and maximum yield attributing characters such as no. of spike (73.38 m-2), spike weight plant-1 (51.20), number of spikelet (256.19) and test weight (45.76 g). Similarly highest grain yield (40.19 q ha-1) and straw yield (62.71 q ha-1), with application of treatment T11 [100 % N & P + PSB + Azotobacter + 100 % K] in comparison to all the treatments. While minimum value of all the growth parameters, yield components and yield were associated with the treatment T1 [Control].

Effect of Bio-fertilizer and Phosphorus on Growth and Yield of Green gram (Vigna radiata L.)

The field experiment was conducted at Crop Research Farm, Naini Agriculture Institute, Department of Agronomy, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Zaid 2022 on sandy loamy soil. The experiment was laid out in Randomized Block Design. The experiment consists of treatments i.e., VAM (20g/kg seed) + Phosphorus 30kg ha-1, PSB (20g/kg seed) + Phosphorus 30kg ha-1, VAM + PSB (40g/kg seed) + Phosphorus 30kg/ha, VAM (20g/kg seed) + Phosphorus 40kg ha-1, PSB (20g/kg seed) + Phosphorus 40kg ha-1, VAM + PSB (40g/kg seed) + Phosphorus 40kg/ha, VAM (20g/kg seed) + Phosphorus 50kg ha-1, PSB (20g/kg seed) + Phosphorus 50kg ha-1, VAM + PSB (40g/kg seed) + Phosphorus 50kg/ha, including control i.e., application of 20-40-20 kg NPK ha-1 (Farmer practice), which are replicated thrice. The variety PDM-139 SAMRAT green gram was sown in February 2023. The results of the experiment revealed that the application of VAM + PSB @ (40g/kg seed) along with 50 kg ha-1 of phosphorus significantly increased the growth parameters viz., plant height (32.94 cm), plant dry weight (42.73 g plant-1), crop growth rate (72.1 g m-2 day-1), relative growth rate (2.16 g m-1 day-1), branches per plant (6.53), nodules per plant (16.4) and yield parameters viz, pods per plant (19.20), seeds per pod (11.87), test weight (40.0g), seed yield (1,620 kg ha-1), haulm yield (1,022.22 kg ha-1), harvest index (49.30%) over control. This treatment also showed its positive effect on economics viz., gross returns (Rs. 1,45,770 ha-1), net return (Rs. 1,04,120.40 ha-1) and benefit cost ratio (2.50).

Influence of Integrated Nutrient Management on the Productivity of kharif Redgram (Cajanus cajan L.)

A field experiment was conducted during kharif season of 2021-22 at Agricultural Research Station, Tandur, Vikarabad, Professor Jayashankar Telangana State Agricultural University (PJTSAU) under deep black soils to study the effect of integrated nutrient management on the growth and yield of kharif redgram (Cajanus cajan L.). The experiment was laid out in Randomized block design with 9 treatments in 3 replications. Treatments comprised of T1: Control, T2: 50% RDF (10:25:0 NPK kg ha-1) + 50% FYM (2.5 t ha-1), T3: 75% RDF (15:37.5:0 NPK kg ha-1) + 25% FYM (1.25 t ha-1), T4: 100% RDF (20:50:0 NPK kg ha-1), T5: 50% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T6: 75% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T7: 100% RDF + FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment), T8: 100% RDF + Rhizobium (Seed treatment) + PSB (Seed treatment) and T9: FYM @ 5 t ha-1 + Rhizobium (Seed treatment) + PSB (Seed treatment). Among the treatments, application of 100% RDF, FYM @ 5 t ha-1, Rhizobium and PSB recorded significantly higher seed yield (1898 kg ha-1) and stalk yield (6275 kg ha-1).

Effect of Seed Treatment with Bioinoculants on Seed Yield and Quality Parameters in Chickpea (Cicer arietinum L.)

The field experiment, was conducted during Rabi 2022 at the field experimental center, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, (U.P). The soil of the experimental plot was sandy loam in texture, nearly neutral in soil reaction (pH 7.1), low in organic carbon (0.36%), available N (171.48 kg/ha), available P (15.2 kg/ha) and available K (232.5 kg/ha). The experiment was laid out in Randomized Block Design with thirteen treatments including control which were replicated thrice. The treatments are as follows, T0- Control, T1 - Bacillus subtilis – 20 g/kg seeds, T2 – Rhizobium – 20 g/kg seeds, T3 – P.S.B – 20 g/kg seed, T4 - T. harzianum – 20 g/kg seed, T5 - T. viridae – 20 g/kg seed, T6 – B. subtilis + Rhizobium – (10 g + 10 g/kg seed), T7 - Rhizobium + P.S.B – (10 g + 10 g/kg seed), T8 - P.S.B + T. harzianum – (10 g + 10 g/kg seed), T9 – T. harzianum + T. viridae – (10 g + 10 g/kg seed), T10 - T. viridae + B. subtilis – (10 g + 10 g/kg seed), T11 - B. subtilis + Rhizobium + P.S.B – (10 g + 10 g+10 g/kg seed), T12 - P.S.B + T. harzianum + T, viridae – (10 g + 10 g+10 g/kg seed) respectively. The experiment results revealed that seeds treated with T7 – Rhizobium + P.S.B – 10 g + 10 g gave better than other treatments viz, days for 50% flowering (60.00), Days to 50% pod setting (75.00) and has matured earlier (99.00), Plant height (74.90 cm), number of pods per plant (52.33), number of seeds per pod (3.00), Seed yield per plot (522.80 g), Biological yield per plot (613.58), Seed index (32.24 gm). Were recorded significantly higher compared to other treatments.

Effect of Organic Manure and Bio Fertilizer on Growth, Yield and Quality in Cabbage

The Department of Horticulture at the Naini Agricultural Institute Sam Higginbottom University of Agriculture, Technology & Sciences, Prayagraj (UP) conducted an experiment in 2022 to investigate the "Effect of organic manures and biofertilizers on growth, yield, and quality in cabbage (Brassica oleraceae L. Var. capitata)". There were ten treatments totaling various amounts of organic manure and bio-fertilizer, with water serving as the control. At 20, 40, and 60 days after transplantation, the treatment is sprayed. The plot size was kept at 2 m × 1.5 m, and each treatment was reproduced three times in a randomized block design. The best results were achieved with treatment T6 (Farm Yard Manure @20t/ha + Bio-Fertilizer (Azotobacter + PSB) each @5kg/ha). The maximum plant height (24.33 cm), the plant's spread (68.59), the number of leaves per plant (13.67), the average head weight (1.93 kg), the head's compactness (5.56 Kg/inch), the yield per plot (6.93 kg), the yield per hectare (71.60 t/ha), the TSS (5.9 Brix), and the amount of ascorbic acid per 100g.Under T6, the gross return (Rs 221430/ha), net profit (Rs 172530/ha), and B:C ratio (4.52) were all much greater. Overall results revealed that the application of FYM @20t/ha + Biofertilizer @5kg/ha proved to be better for different growth, yield and quality traits in cabbage.

Fruit Characteristics and Yield of Mango cv. Amrapali as Influenced by Bio-enhancers and Bio-fertilizers

To study the “Fruit Characteristics and Yield of Mango cv. Amrapali as Influenced by Bio-enhancers and Bio-fertilizers” a field experiment was conducted in the Garden, Department of Horticulture, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur (U.P.) during 2020-2021. The experiment consists of seven treatments i.e., FYM (25 kg/tree/year) + Organic mulch (Paddy straw) i.e., Control, FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Amritpani (20%) + Azotobacter (100 g/tree), FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Panchagavya (3%) + Azotobacter (100 g/tree), FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Jivamrit (20%) + Azotobacter (100 g/tree), FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Amritpani (20%) + PSB culture (100 g/tree), FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Panchagavya (3%) + PSB culture (100 g/tree), FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Jivamrit (20%) + PSB culture (100 g/tree) which were replicated thrice in randomized block design by using one plant as a unit per treatment. The application of different bio-enhancers and bio-fertilizers to mango plant was made as per the requirement of the treatment.The experimental results clearly revealed that the plants treated with the application of FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Panchagavya (3%) + Azotobacter (100g/tree) produced fruits with significantly higher length, width, weight, volume, pulp weight, pulp:peel ratio with more yield. However, the fruit with minimum fruit length, width, weight, volume, pulp weight, pulp:peel ratio, and yield were recorded from the plants treated with the application of FYM (25 kg/tree/year) + Organic mulch (Paddy straw) i.e., Control. Thus, on the basis of the above observations, this can be suggested that the application of FYM (25 kg/tree/year) + Organic mulch (Paddy straw) + Panchagavya (3%) + Azotobacter (100 g/tree) were effective for substantially higher physical fruit characteristics and yield under the sub-tropical plains of Central Uttar Pradesh, India.

Effect of Different Sources of Nutrients on Growth, Yield and Quality of Mung Bean (Vigna radiata L.)

A pot experiment was conducted in the experimental research farm of Department of Soil Science, SAS, Nagaland University, and Medziphema campus, Nagaland in the period of June to September 2022 to make a study on “Effect of different sources of nutrients on growth, yield and quality of mung bean (Vigna radiata L.). The experiment was laid out in Complete Randomized Design (CRD) with 12 treatments and 3 replications. The study revealed that the incorporation of different sources of nutrients significantly influenced that the plant growth, yield attributes and quality parameters i.e., NPK and protein content, Nutrient uptake, and availability of nutrients in soil after harvest of green gram. The crop growth attributes such as plant height, number of leaves plant-1 and number of branches plant-1 were significantly influenced by its application. The highest seed yield of 11.67 g pot-1and stover yield of 37.88 g pot-1 recorded with the treatment of 100% RDF+PSB+FYM was significantly superior over all treatments. The quality of green gram was improved by 100% RDF+PSB+FYM in the presence of different sources of nutrients. The highest nutrient content and highest nutrient uptake by green gram was also recorded highest with the treatment 100% RDF+PSB+FYM. The available nutrients (NPK), cation exchange capacity, organic carbon, soil respiration, microbial biomass carbon in soil was also found to be more due to the treatment of 100% RDF+PSB+FYM.

Influence of Cropping System and Nutrient Management Practices on the Yield, and Economics of Pearl Millet [Pennisetum glaucum]

The Field experiments were conducted to evaluate the effect of cropping systems and nutrient management practices on the yield, and economics of pearl millet in the School of Agricultural Science, Karunya Institute of Technology and Sciences, Coimbatore, India). The experiments were laid out in Factorial Randomized Block Design (FRBD) with two factors (Cropping system and Nutrient management) and three replications for two consecutive seasons in 2022-23. The study comprised three cropping system treatments namely C1 – Sole Pearl millet cropping, C2 - Pearl millet + Black gram intercropping (1:1), C3 – Pearl millet + Cowpea intercropping (1:1) along with four nutrient management treatments viz., N1 - 100% Recommended dose of fertilizer (RDF) @80:40:40 kg of N, P2O5, and K2O/ha., N2– 75% RDF + Azospirillum @2kg/ha and Phosphate solubilizing bacteria (PSB) @2kg/ha, N3 – 75% RDF + Soil application of Azospirillum (2kg/ha) and PSB (2kg/ha) + Foliar spray of 2% urea @ 15 and 35 days after transplanting (DAT), N4 – 75% RDF + Soil application of Azospirillum (2kg/ha) and PSB (2kg/ha) + Foliar spray of Panchagavya3% @ 15 and 35 DAT. The results of the experiment revealed that the intercropping of black gram or cowpea in pearl millet proves to be beneficial and advantageous in terms of, grain yield and returns per rupee invested, rather than going for the sole cropping of pearl millet. On the other hand, reduced application of inorganic recommended dose of fertilizers by 25% with the inclusion of biofertilizers like Azospirillum and phosphate solubilizing bacteria, along with the foliar application of 2% urea or 3% Panchagavya will be an economically viable and environmentally sustainable nutrient management practice for improving the yield of pearl millet.

Growth and Yield Response of Cluster Bean (Cyamopsis tetragonoloba L.) cv. Pusa Navbahar to Integrated Nutrient Management

The investigation on cluster bean with application of integrated nutrient management with comprised of 12 treatments with three level of NPK, two levels of PSB and Rhizobium in factorial randomized block design. The treatment T12 has shown the significant results when applied 100% of NPK with PSB and Rhizobium among the different levels of treatment combinations. Growth parameters viz., plant height (90.96 cm), number of nodules plant-1(59.48), number of branches plant-1 (2.66) at 90 DAS (day after sowing), and yield parameters viz., number of clusters plant-1 (14.62), number of pods cluster-1 (13.39), pod length (17.42 cm),pod yield(77.29 q ha-1) has shown best in treatment T12(NPK @100% + PSB @100% + Rhizobium @100%) of cluster bean (Cyamopsis tetragonoloba L.) in comparison to other treatment combination.

Impact of Fertigation Regimes on Quality of Ginger (Rio-de-Janeiro and Himachal)

Ginger is used as one of the important ingredients in traditional as well as modern medicine besides as a spice. It boosts immunity and is a rich source of many biologically active substances and minerals. Although it is a medicinally important crop, its productivity is, however, affected due to poor nutrient management and therefore it requires an adequate supply of nutrients in the form of inorganic fertilizers or organic manuring, or a mixture of both. In this context, the present study was aimed to investigate the effect of fertigation regimes on quality of ginger (Rio-De-Jenereo and Himachal) at vegetable division in department of Horticulture, Gandhi Krishi Vignana Kendra, University of Agricultural Sciences, Bangalore. The experiment comprised of eleven treatments, Sources of nutrients used were FYM (Farm Yard Manure), Azotobacter, PSB (Phosphate Solubilising Bacteria), AMF (Arbuscular Mycorrhizal Fungi), KMB (Potassium Mobilizing Bio fertilizer), Inorganic sources (Urea, DAP, MOP, SSP, Ginger special, Neem cake). The results showed that the application of 200 % RDF (200:100:100 N: P2O5: K2O kg/ha) fertigation + FYM 30 t/ha, Neem cake 2 t/ha] was found effective for increment in essential oil (%), oleoresin content (%), gingerol (%) and crude fibre (%) in both varieties of ginger among the all treatments evaluated in this zone (Bangalore).

Effect of Different Sources of Phosphorus on Protein Content, Soil Fertility and Yield of Greengram

To study the effect of different sources of phosphorus on quality, fertility status and yield of greengram, an experiment was conducted during the kharif season of 2014 at Crop Research Farm, Department of Agronomy, SHIATS, Allahabad, UP. The experiment was conducted in randomized block design with 9 treatments viz. Control (No Phosphorus, 100% P through SSP, 100% P through URP, 50% P through SSP + 50% P through URP, 75% P through SSP + 25% P through URP, 100% P through SSP + PSB inoculation, 100% P through URP + PSB inoculation, 50% P through SSP + 50% P through URP + PSB inoculation, 75% P through SSP + 25% P through URP + PSB inoculation. Greengram variety Samrat was sown at a spacing of 30cm x 10 cm and it was fertilized with Phosphorus, SSP, URP, PSB as per the requirement of the treatments. The experimental results revealed that application of 100% P through SSP + PSB seed inoculation produced significantly higher grain yield (1496.77 kg ha-1), Stover yield (2920 kg ha-1) and protein (24.06%). There was a steady increase in the soil fertility status after harvesting of greengram. However, maximum available P2O5 (36.07 kg ha-1), available N (329.03 kg ha-1), available K2O (220.80 kg ha-1) was obtained with the application of 100% P through SSP + PSB seed inoculation. It also recorded higher net returns (Rs 47002.17 ha-1) and Benefit Cost (1.89). Thus, application of 100% P through SSP + PSB seed inoculation) helps in improving the quality and enhancing the yield in Greengram for resource poor farmers of India.

Influence of Different Organic Sources of Nutrients on Yield and Quality of Pomegranate (Punica granatum L.) cv. Bhagwa

A field experiment entitled, Influence of different organic sources of nutrients on yield and quality of pomegranate (Punica granatum L.) cv. Bhagwa. The current study was designed in RBD with three replications consisting of twenty-two treatments with four different organic manures viz., farmyard manure, vermicompost, poultry manure and neem cake as a source of nitrogen and recommended dose of manure and fertilizers based on plant age with or without biofertilizers (Azotobacter, PSB & KMB) and biopesticides (Trichoderma viride and and Paecilomyces lilacinus). The findings based on pooled data discovered that maximum number of fruits per plant (59.50), yield per plant (12.41 kg), yield per hectare (19.86 tonne), fruit weight (239.39 g), fruit diameter (7.55 cm), aril weight per fruit (147.70 g), juice content per fruit (97.55 ml), peel weight per fruit (91.69 g) along with peel: aril ratio (0.62) was significantly noted under treatment 100 % RDN through poultry manure + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus. The maximum net return (4,12,840/ha) and BCR (3.26) were also obtained in same treatment. Significantly maximum TSS (16.15°Brix), reducing sugar (14.44 %), non-reducing (2.04 %) and total sugar (16.48 %) along with minimum titrable acidity (0.41 %) were recorded with application of 37.5 % RDN through FYM + 37.5 % RDN through neem cake + 50 ml Azotobacter culture + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus treatment. Whereas, maximum ascorbic acid content (16.72 mg/100 ml juice) was significantly found under treatment 100 % RDN through FYM + 50 ml PSB + 25 ml KMB + 5 g Trichoderma viride + 5 ml Paecilomyces lilacinus.

PSB0739 inhibits formation of semen-derived amyloid fibril / 南方医科大学学报

OBJECTIVE@#To explore the inhibitory effect of PSB0739 on the formation of semen-derived amyloid fibrils.@*METHODS@#PAP248-286 (440 μmol/L) was incubated with PSB0739 at different concentrations, and at different time points of incubation, aliquots were taken from each sample for Congo red staining to detect the formation of amyloid fibers. The morphology of amyloid fibrils incubated in the presence or absence of PSB0739 was visualized using transmission electron microscope. The effect of PSB0739 on amyloid fibril formation was determined using virus infection assays at different time points, and the surface charges of amyloid fibril incubated with PSB0739 were calculated using a Zeta potentiometer. The cytotoxicity of PSB0739 in Hela cells was determined using MTT assay. The antiviral effect of PSB0738 against HIV- 1 was evaluated by infection assay.@*RESULTS@#PSB0739 inhibited SEVI fibril formation in a dose-dependent manner . At 48 h of incubation, 220 μmol/L of PSB0739 obviously inhibited the formation of amyloid fibrils in 440 μmol/L of SEVI. Transmission electron microscopy revealed that 220 μmol/L PSB0739 prevented PAP248- 286 (440 μmol/L) from forming amyloid fibrils. PSB0739 antagonized SEVI-mediated enhancement of HIV-1 infection, and 1760 μmol/L of PSB0739 completely reversed the positive charge of SEVI ( < 0.05). PSB0739 below the concentration of 62.5 μmol/L showed no obvious cytotoxicity in Hela cells (>0.05). PSB0739 showed a direct anti-HIV activity with an IC of 21.77±5.15 μmol/L.@*CONCLUSIONS@#PSB0739 can inhibit the formation of semen-derived amyloid fibrils .

Role of phosphate solubilizing bacteria on rock phosphate solubility and growth of aerobic rice.

Use of phosphate-solubilizing bacteria (PSB) as inoculants has concurrently increased phosphorous uptake in plants and improved yields in several crop species. The ability of PSB to improve growth of aerobic rice (Oryza sativa L.) through enhanced phosphorus (P) uptake from Christmas island rock phosphate (RP) was studied in glasshouse experiments. Two isolated PSB strains; Bacillus spp. PSB9 and PSB16, were evaluated with RP treatments at 0, 30 and 60 kg ha-1. Surface sterilized seeds of aerobic rice were planted in plastic pots containing 3 kg soil and the effect of treatments incorporated at planting were observed over 60 days of growth. The isolated PSB strains (PSB9 and PSB16) solubilized significantly high amounts of P (20.05-24.08 mg kg-1) compared to non-inoculated (19-23.10 mg kg-1) treatments. Significantly higher P solubilization (24.08 mg kg-1) and plant P uptake (5.31 mg plant -1) was observed with the PSB16 strain at the highest P level of 60 kg ha-1. The higher amounts of soluble P in the soil solution increased P uptake in plants and resulted in higher plant biomass (21.48 g plant -1). PSB strains also increased plant height (80 cm) and improved root morphology in aerobic rice. The results showed that inoculation of aerobic rice with PSB improved phosphate solubilizing activity of incorporated RP.

The Role of Blind Protected Specimen Brushing (PSB) in Intubated Patients / 결핵

BACKGROUND: In intubated patients, cultures of endotracheal aspirates (EA) are apt to contamination throughout the endotracheal tube. Therefore, the identification of etiologic agents via conventional EA cultures is not always reliable. In order to differentiate a pulmonary infection from a non-infectious disease, and to identify the true etiologic agent of acute pulmonary infection, blinded protected specimen brushing (PSB) was used, and its efficacy evaluated. METHODS: In 51 intubated patients, with suspected pneumonia, blind PSB were performed, and the results compared with blood and EA cultures. A protected specimen brush was introduced through the endotracheal tube, and settled at the affected large bronchus. A specimen brush was introduced to the expected region using the blind method. The tip of the brush was introduced with an aseptic technique after vigorously mixed for 1 minute in 1cm3 of Ringer's lactate solution. The specimens were submitted for quantitative culture within 15 minutes, with a culture being regarded as positive if the colony forming units were above 103/ml. RESULTS: Of the 51 patients, 15 (29.4%) had community-acquired pneumonia (CAP), 27 (52.9%) hospital-acquired pneumonia (HAP) and 9 (17.6%) non-infectious diseases. The sensitivity and specificity of the quantitative PSB culture for the diagnosis of pneumonia were 52.4 and 88.9%, respectively. The sensitivity and specificity of EA were 78.6 and 77.8%, respectively. The blind PSB was superior to the EA for the identification of true etiologic agents. Of 53 episodes of 27 HAP patients, MRSA (Methicillin-resistant staphylococcus aureus) (41.5%) was the most common causative agent followed by Pseudomonas aeruginosa (15.1%), Klebsiella sp. (7.5%) and Acinetobacter sp. (7.5%). CONCLUSIONS: As a simple, non-invasive diagnostic modality, the blind PSB is a useful method for the differentiation of a pulmonary infection from non-infectious diseases and to identify the etiologic agents in intubated patients. A blind PSB can be performed without bronchoscopy, so is safer, more convenient and cost-effectiveness for patients where bronchoscopy can not be performed.