HCV infection status and risk factors in remunerated blood donors in Jiangsu province / 中华流行病学杂志

Objective To investigate the infection status of HCV in remunerated blood donors and risk factors in Jiangsu province.Methods A Cross-sectional study was conducted among people aged ＞50 years.Questionnaires were used to collect the information about their demographic characteristics and risk behaviors,and venous blood samples were collected from them to detect HCV anti-body,HCV-RNA and other biochemical indicators.EpiData and Stata were used for data entry and statistical analysis.Results The overall HCV sero-prevalence rates were 22.55％ and 61.05％ among remunerated blood donors.Data firom multiple stepwise regression analysis showed that alanine aminotransferase (ALT) (adjusted OR=1.38,95％CI:1.18-1.62) and aspartate aminotransferase (AST) (adjusted OR=l.30,95％CI:1.10-1.54) were associated with the outcomes of HCV infection,and fasting plasma glucose (adjusted OR=1.17,95％CI:1.01-1.35) were associated with HCV RNA viral loads.Conclusion The prevalence of HCV infection in remunerated blood donors was high,clinical ALT,AST and fasting plasma glucose levels were associated with the risk for HCV infection and HCV RNA viral load.

HCV infection status and risk factors in remunerated blood donors in Jiangsu province / 中华流行病学杂志

Objective To investigate the infection status of HCV in remunerated blood donors and risk factors in Jiangsu province.Methods A Cross-sectional study was conducted among people aged ＞50 years.Questionnaires were used to collect the information about their demographic characteristics and risk behaviors,and venous blood samples were collected from them to detect HCV anti-body,HCV-RNA and other biochemical indicators.EpiData and Stata were used for data entry and statistical analysis.Results The overall HCV sero-prevalence rates were 22.55％ and 61.05％ among remunerated blood donors.Data firom multiple stepwise regression analysis showed that alanine aminotransferase (ALT) (adjusted OR=1.38,95％CI:1.18-1.62) and aspartate aminotransferase (AST) (adjusted OR=l.30,95％CI:1.10-1.54) were associated with the outcomes of HCV infection,and fasting plasma glucose (adjusted OR=1.17,95％CI:1.01-1.35) were associated with HCV RNA viral loads.Conclusion The prevalence of HCV infection in remunerated blood donors was high,clinical ALT,AST and fasting plasma glucose levels were associated with the risk for HCV infection and HCV RNA viral load.

Men who have sex with men and its relationship with HIV-1 strains prevailing in the paid blood donors from Zhengzhou city, Henan / 中华流行病学杂志

Objective To clarify the genetic characteristics of human immunodeficiency virus (HIV) circulated in the population of men who have sex with men (MSM) in Zhengzhou,Henan and to analyze its relationship with HIV-1 prevailing in the paid blood donors (PBDs).Methods Thirty-one MSM who were confirmed as HIV positive individuals in 2010 together with 41 HIV-positive former PBDs were enrolled in the study.Information on related epidemiological characteristics and their plasma were collected.RT-PCR was used to amplify HIV-1 full length gag (1584 bp),pol (3147 bp) genes and partial env gene (C2V3 segment,558 bp) followed by sequencing on those subjects.Online software available at LosAlamos HIV Database was used to identify the HIV subtypes based on the findings of the sequences.Phylogenetic tree was used to identify thc possible relationship of transmission.Results Fifty-three full length gag,38 full length pol and 48 partial env (C2V3) genes were collected from 72 participants.Among the 31 HIV ( + )MSM individuals,14 CRF01_AE strains,5 CRF07BC_ strains and 12 subiype B ( 1 subtype B and 11B' ) strains were identified respectively.All of the 41 strains identified from former PBDs were infected by B' strains.The CRF01_AE strains identified in MSM showed a close relationship to those identified from both Hebei and Liaoning provinces.The CRF07 BC strains showed a close relationship with those from Shijiazhuang and Beijing cities.Among the 12 subtype B strains,8 sequences grouped into 1 cluster with 1 sequence from the former PBDs.Two sequences grouped with 02HNseq4 suggested that B' had been prevailed in the MSM population might come from the former PBDs and were closely related to the strains identified in the MSM population.Conclusion Complicated genetic background and multiple introductions of HIV in the MS population in Zhengzhou,were found.This was also the first report which noticed that the subtype B epidemic among Zhengzhou MSM was mainly originated from the B' among the former PBDs.

Impact of delayed diagnoses bias on the estimation of AIDS incubation / 中华流行病学杂志

Objective Using the HIV positive paid blood donors (PBDs) as example, to investigate the impact of the delayed diagnosed HIV/AIDS patients on the calculated median incubation of AIDS. Methods Information was retrospectively collected on HIV/AIDS patients who were infected through paid blood donation. Data was from the national HIV/AIDS case reporting system. The time of diagnosed HIV/AIDS after the screening was defined as delayed diagnoses. All the cases were diagnosed between Jan. 1st 1995 and the end of the screening were classified as group 1, while the cases diagnosed between Jan. 1st 1995 and Dec. 31st 2010 were classified as group 2.Kaplan-Meier method was used to calculate the median AIDS incubation of cases from the two groups. Results from the two groups on theft incubation periods were compared. Results From the data of group 1, we noticed that the median AIDS incubation was 9.62 years but the 95％ CI were not provided in the results of SAS software. When using the data from group 2,we found that the median AIDS incubation was 9.83 years, with the 95％CI as 9.73 to 9.83 years. Conclusion Our data showed that, if excluding the delayed diagnosed cases, the AIDS incubation periods were shorter and biased.

Donadores sanguíneos remunerados: un nuevo grupo de riesgo para desarrollar SIDA en México / Paid blood donors: a high risk group for AIDS in Mexico

Se analizan las características epidemiológicas y clínicas de 7 pacientes con SIDA contraído a través de agujas y equipos para sangría contaminados; en estos pacientes no se demostró otro factor de riesgo conocido. En la Dirección de Epidemiología de los Servicios Coordinados de Salud en el estado de Puebla se notificaron 37 casos de SIDA hasta el 15 de junio de 1988; en este grupo, el 19 por ciento fueron donadores remunerados de sangre y/o plasma. Cinco fueron del sexo masculino y dos del femenino; su edad varió entre 26 y 45 años. Sus datos clínicos fueron: pérdida de peso, fiebre, diarrea crónica, linfadenopatía en siete pacientes, en seis patología respiratoria, en cuatro condidiasis, en dos Tb pulmonar. En la dirección de Epidemiología se tienen notificados 517 donadores sanguíneos remunerados portadores de Virus de Inmunodeficiencia Humana, (VIH). No se conoce el número de donacciones de sangre y por ende a los receptores. El SIDA postranfusional en Puebla suma el 52 por ciento de los casos y se espera que esta cifra irá en aumento. Proponemos se incluya en un nuevo grupo de riesgo a los donadores sanguíneos remunerados

Subgenotype Analysis of Hepatitis C Virus in paid Biood Donors / 中国输血杂志

The detecting of HCV-RNA and genotyping of HCV in sera of 65 paid blood donors which are positive for anti-HCV were conducted,indicating that 60 of them appeared positive for HCV-RNA,and their genotype of HCV was classified as genotype- I . It was suggested that the genotype- I of HCV could be considered as the predominant infectious strain in this group of paid blood donors. It was found that the endonuclease digestion method of typing based on HCV 5' -noncoding region PCR was better compared with the direct method of typing based on HCV core region PCR in terms of its senstivity,economy and simplicity.