The effect of different guanine base number on fluorescence intensity of DNA/ silver nanoclusters / 药学实践杂志

Objective To investigate the effect of different guanine base numbers on the fluorescence intensity of DNA/ silver nanoclusters through C-G base complementary pairing, in order to explore a new method for the construction of fluorescent probe switches. Methods Designed complementary sequences of aptamer parts of a series of silver nanoclusters by using the nucleic acid base complementary pairing principle, and investigated the effect of the number of G bases exposed in the aptamer sequence on the fluorescence signal. Results Base G could enhance the fluorescence signal intensity of silver nanoclusters, and the fluorescence signal strength was positively correlated with the number of G bases. The fitting linear equation was Y=1726.1X+8972.5, r=0.9789. Conclusion This study is a great reference for the regulation of fluorescence intensity of silver nanoclusters and the design of G quadruplet aptamer fluorescent probe switch.

Nomeação Bidirecional em Crianças com Autismo: Efeitos dos Procedimentos de Observação de Pareamento de Estímulos e Instrução com Múltiplos Exemplares / Bidirectional Naming in Children with Autism: Effects of Stimulus Pairing Observation Procedure and Multiple Exemplar Instruction

RESUMO: Este estudo comparou a eficiência dos procedimentos de Instrução com Múltiplos Exemplares (MEI) e de Observação de Pareamento de Estímulos (SPOP) para estabelecer nomeação bidirecional (BiN) em quatro crianças com Transtorno do Espectro do Autismo. Foi utilizado um delineamento de sondas múltiplas entre pares de participantes. Um participante demonstrou emergência de BiN completa, após passar pelos dois tratamentos experimentais (primeiro SPOP, depois o MEI). Dois participantes demonstraram emergência somente do componente de ouvinte, após o SPOP. No teste final de nomeação, dois participantes demonstraram emergência somente do componente de ouvinte da BiN, e um participante apresentou aumento nos dois componentes em relação à linha de base. Discute-se a eficiência dos tratamentos de maneira isolada, quando apresentados em sequência, além da importância do uso de estímulos reforçadores no processo de aquisição da BiN.

ABSTRACT: This study compared the efficiency of Multiple Exemplar Instruction (MEI) and Stimulus Pairing Observation Procedure (SPOP) for establishing bidirectional naming (BiN) in four children with Autism Spectrum Disorder. A multiple probe design was used between pairs of participants. One participant showed emergence of complete BiN after having undergone the two experimental treatments (first SPOP, then MEI). Two participants demonstrated emergence of the listener component after undergoing SPOP treatment. During the final naming test, two participants demonstrated emergence only for the BiN listener component, and one participant showed an increase in both components in relation to the baseline. The efficiency of treatments in isolation, when presented in sequence, is discussed, in addition to the importance of using reinforcing stimuli in the BiN acquisition process.

Aquisição de intraverbais em crianças com autismo: efeitos do pareamento de estímulos e respostas ecoicas / Intraverbal acquisition in autistic children: effects of stimulus pairing and echoic responses / Acquisition du comportement intraverbal chez des enfants autistes: effets de l'appariement des stimuli et des réponses échoïques / Adquisición de intraverbales en niños con autismo: efectos del emparejamiento de estímulos y respuestas ecoicas

Resumo O ensino de intraverbais para indivíduos com autismo tem sido realizado, principalmente, através de procedimentos que envolvem reforçamento diferencial. Recentemente, o procedimento de observação de pareamento de estímulos (SPOP) surgiu enquanto alternativa para verificar a emergência de repertórios verbais. O SPOP implica o pareamento de estímulos sem exigir outra resposta que a observação do pareamento. O ecoico tem sido apontado como facilitador na emergência de operantes verbais. O presente estudo comparou, com um delineamento de sondas múltiplas concorrentes com tratamento alternado adaptado, a eficácia do SPOP com um procedimento de apresentação contígua de estímulos com requisição de resposta ecoica na emergência de intraverbais em três crianças com autismo. Observou-se a eficácia do SPOP na indução de intraverbais e verificou-se o papel facilitador do ecoico. Discutem-se possíveis efeitos de interferência dos tratamentos, da extinção de respostas no contexto experimental e da utilização de reforçadores condicionados como parte do SPOP.

Abstract Intraverbals have been taught to individuals with autism primarily through procedures involving differential reinforcement. Recently, Stimulus Pairing Observation Procedure (SPOP) emerged as an alternative to verify the emergence of verbal repertoires. The SPOP consists in the simultaneous presentation of two stimuli and requires only the observation response by the participant. The echoic behavior had been pointed out as a mediator in the emergence of verbal operants. This study compared the efficacy of the SPOP and of a contiguous stimulus presentation procedure with an echoic response requirement in emergence of intraverbals in three children with autism by using a concurrent multiple probe design with an adapted alternating treatment. The results showed the SPOP's efficacy for intraverbals induction and suggested a facilitating role of the echoic. A possible carry-over effect between treatments, extinction in the experimental context and the use of conditioned reinforcers in SPOP are discussed.

Résumé L'enseignement de l'intraverbal chez des individus autistes a été principalement réalisé avec des procédures impliquant le renforcement différentiel. Plus récemment, la Procédure d'Observation de Paires des Stimuli (SPOP en anglais) s'est imposée comme alternative pour vérifier l'émergence de répertoires verbaux. Le SPOP implique l'appariement des stimuli sans exiger d'autre réponse que l'observation des paires. Par ailleurs, l'échoïque a été signalé comme facilitateur de l'émergence d'opérants verbaux. Nôtre étude a comparé, avec un plan expérimental de sondes multiples concurrents et traitements alternés adapté, l'efficacité du SPOP en comparaison à une procédure de présentation du stimulus contigu avec réponse échoïque dans l'émergence du comportement intraverbal chez trois enfants autistes. Nous avons pu observer l'efficacité du SPOP dans l'induction de l'intraverbal ainsi qu'un rôle facilitateur de l'échoïque. Nous discutons les effets possibles de l'interférence entre traitements, de l'extinction de réponses dans le contexte expérimental, et de l'emploi de renforçateurs conditionnés dans le cadre du SPOP.

Resumen La enseñanza de intraverbales a autistas se ha realizado principalmente a través de procedimientos con refuerzo diferencial. Más recientemente, el procedimiento de observación de emparejamiento de estímulo (POEE) surgió como alternativa para verificar la emergencia de repertorios verbales. El POEE implica el emparejamiento de estímulos, sin exigir otra respuesta que la observación del emparejamiento. El ecoico se considera facilitador en la emergencia de operantes verbales. Este estudio comparó, desde un delineamiento de sondas múltiples con tratamiento alternado adaptado, la eficacia del POEE con un procedimiento de presentación de estímulo contiguo solicitante de respuesta ecoica en la emergencia de intraverbales en tres niños autistas. El POEE fue eficaz en la inducción de intraverbales, y el ecoico tuvo un papel facilitador. Se discuten los posibles efectos de interferencia de los tratamientos, de la extinción de respuestas en el contexto experimental y de la utilización de reforzadores condicionados como parte del POEE.

Ion-pairing liquid chromatographic method determination of ketoconazole and stress degradation in pharmaceutical formulations with fluorescence detection

Ion-pairing liquid chromatographic method was validated for determination of ketoconazole in shampoo and cream samples as per ICH guidelines. The chromatographic conditions were carried out in the isocratic mode using a mixture of methanol and 8 mM sodium dodecyl sulfate (pH 5.5) in a ratio of 45:55 v/v %, as mobile phase. The flow rate was set at 1.0 mL min-1. Chromolith RP-18e (100×4.6 mm) was used as the analytical column with a fluorescence detection at an excitation wavelength of 260 nm and an emission wavelength of 375 nm. The average percentage recovery of shampoo A, shampoo B, shampoo C, cream A and cream B were 99.88, 97.06, 99.58, 96.77 and 97.26, respectively. The limit of detection was 0.12 mg L-1. The drug decomposition under acid degradation, base degradation and oxidative degradation were found to be in the range of 91.63-94.70% indicating that the drug is resistant towards acidic conditions. The drug decomposition under thermal condition and photolysis condition were found to be in the range of 69.05-87.15% and 47.31-66.83% respectively, indicating that the drug decomposition is more sensitive under photolysis conditions. This method is suitable for the quality control of ketoconazole in commercial shampoo and creams.

Genome size in three species of Glandularia and their hybrids / Tamaño del genoma en tres especies de Glandularia y sus híbridos

In this work the relationship between genome size of Glandularia species and the meiotic configurations found in their hybrids are discussed. Glandularia incisa (Hook.) Tronc., growing in two localities of Corrientes and Córdoba provinces, Argentina, with different ecological conditions, showed inter-population variability of the 2C-value. The DNA content found in the Corrientes locality (2.41 pg) was higher than that obtained in the Córdoba locality (2.09 pg) which has more stressful environmental conditions than the former. These values are statistically different from those that were found in Glandularia pulchella (Sweet) Tronc. from Corrientes (1.43 pg) and in Glandularia perakii Cov. et Schn from Córdoba (1.47 pg). The DNA content of the diploid F1 hybrids, G. pulchella × G. incisa and G. perakii × G. incisa, differed statistically from the DNA content of the parental species, being intermediate between them. Differences in the frequency of pairing of homoeologous chromosomes were observed in the hybrids; these differences cannot be explained by differences in genome size since hybrids with similar DNA content differ significantly in their meiotic behavior. On the other hand, the differences in the DNA content between the parental species justify the presence of a high frequency of heteromorphic open and closed bivalents and univalents with different size in the hybrids.

En el presente trabajo se discute la relación entre el tamaño del genoma en especies de Glandularia y las configuraciones meióticas encontradas en sus híbridos. El valor 2C mostró variabilidad interpoblacional en muestras de Glandularia incisa (Hook.) Tronc. coleccionadas en dos localidades con diferentes condiciones ecológicas (provincias de Corrientes y Córdoba, Argentina). El contenido de ADN encontrado en Corrientes (2,41 pg) fue mayor que el obtenido en Córdoba (2,09 pg) donde se registran condiciones ambientales más estresantes. Estos valores son estadísticamente diferentes de los determinados en Glandularia pulchella (Sweet) Tronc. de Corrientes (1.43 pg) y en Glandularia perakii Cov. et Schn de Córdoba (1.47 pg). El contenido de ADN de los híbridos diploides F1, G. pulchella × G. incisa y G. perakii × G. incisa, difirió estadísticamente del contenido de ADN registrado en las especies parentales siendo intermedio entre ellas. Las diferencias observadas en la frecuencia de apareamiento de cromosomas homeólogos no pueden explicarse por diferencias en el tamaño del genoma, ya que híbridos con un contenido de ADN similar difieren significativamente en su comportamiento meiótico. Sin embargo, la diferencia en el contenido de ADN entre las especies parentales explica la presencia de una alta frecuencia de bivalentes heteromórficos tanto abiertos como cerrados y univalentes con diferentes tamaños.

Reproductive strategies of a population of a freshwater amphipod (Crustacea, Amhipoda, Hyalellidae) from southern Brazil / Estratégias reprodutivas de uma população de um anfípodo de água doce (Crustacea, Amhipoda, Hyalellidae) do sul do Brasil

Abstract: The aim of this study was to characterize the reproductive strategies of a population of Hyalella bonariensis from southern Brazil. Pairing success, reproductive period of males and females, fecundity, and body size at the onset of reproduction were evaluated. Animals were sampled four times (August 2012, October 2012, January 2013, and April 2013) with the 250 µm-mesh dip net during 20 minutes by only one person. In the field, precopulatory pairs and ovigerous females were individualized. In the laboratory, cephalothorax length (CL) were measured and eggs and juveniles were removed from the females' marsupium and counted. The mean CL of paired males and females was significantly higher than that of non-paired males and females. A sexual dimorphism in body size was observed in the population - both paired and non-paired males were larger than females. Probably larger males have a higher probability of losing females during precopulatory behavior. A significant correlation was observed between the size of paired males and females - larger males often paired with larger females and smaller males paired with smaller females (r = 0.81). The pairing success of males increased with body size and we can assume that males from all size classes are able to find mates. The pairing success of females was independent of body size - paired and non-paired females had similar mean CL. The reproductive success increased with body size in males and females, and was more evident in males. Females from the largest size classes had null reproductive success. The idea that larger females can produce more eggs but might have a lower probability of finding a mate than smaller females was corroborated by our results. Therefore, smaller females have higher pairing success because they are capable of mating with a higher percentage of males. The estimated mean fecundity of H. bonariensis was 17.4 (± 3.89) eggs/juveniles. Our results are similar to those of other species of Hyalella from Brazil.

Resumo: Este trabalho teve por objetivo caracterizar as estratégias reprodutivas de uma população de Hyalella bonariensis no sul do Brasil, avaliando o sucesso de pareamento, o período reprodutivo de machos e fêmeas, a fecundidade e o tamanho corporal dos indivíduos durante a reprodução. Para isso foram realizadas quatro amostragens (Agosto/2012, Outubro/2012, Janeiro/2013 e Abril/2013) utilizando-se uma rede de mão, com malha de 250 µm, durante 20 minutos por um coletor. Em campo, os casais e as fêmeas ovígeras foram individualizados e em laboratório os mesmos foram mensurados quanto ao comprimento do cefalotórax (CC) (mm) e os ovos ou juvenis encontrados dentro do marsúpio foram contados. O CC médio dos machos e fêmeas pareados foi significativamente superior ao dos machos e fêmeas não pareados. Um dimorfismo sexual no tamanho corpóreo foi observado na população - ambos machos pareados e não pareados foram maiores do que as fêmeas. Aparentemente os machos maiores têm uma maior probabilidade de perder fêmeas durante o comportamento precopulatório. Foi observada correlação positiva entre o tamanho dos machos e fêmeas que foram encontrados formando casais - machos maiores geralmente pareiam com fêmeas maiores e machos menores com fêmeas menores (r=0,81). O sucesso de pareamento dos machos aumentou com o tamanho corpóreo e podemos assumir que os machos de todas as classes de tamanho são capazes de encontrar parceiras sexuais; entretanto, o sucesso de pareamento das fêmeas não depende do tamanho das mesmas, sendo que as fêmeas pareadas e não pareadas apresentaram CC médio semelhante. O sucesso reprodutivo da espécie aumentou com o tamanho corpóreo em machos e fêmeas, sendo mais pronunciado nos machos. As fêmeas pertencentes às maiores classes de tamanho apresentaram sucesso reprodutivo nulo. Os resultados suportam a ideia que as fêmeas grandes poderiam ter uma baixa probabilidade de encontrar parceiro para formar par pré-copulatório, quando comparada às fêmeas de tamanhos inferiores, apesar das fêmeas maiores serem mais fecundas. Neste sentido, as menores fêmeas possuem um sucesso de pareamento maior, pois elas são capazes de copular com uma elevada proporção de machos. A fecundidade média da espécie foi estimada em 17,4 (± 3,89) ovos/juvenis. Os resultados observados no presente trabalho assemelham-se aos encontrados para outras espécies do gênero já analisadas no sul do Brasil.

Arbuscular mycorrhizal fungi in Mimosa tenuiflora (Willd. ) Poir from Brazilian semi-arid

Abstract Many plant species from Brazilian semi-arid present arbuscular mycorrhizal fungi (AMF) in their rhizosphere. These microorganisms play a key role in the establishment, growth, survival of plants and protection against drought, pathogenic fungi and nematodes. This study presents a quantitative analysis of the AMF species associated with Mimosa tenuiflora, an important native plant of the Caatinga flora. AMF diversity, spore abundance and root colonization were estimated in seven sampling locations in the Ceará and Paraíba States, during September of 2012. There were significant differences in soil properties, spore abundance, percentage of root colonization, and AMF diversity among sites. Altogether, 18 AMF species were identified, and spores of the genera Acaulospora, Claroideoglomus, Dentiscutata, Entrophospora, Funneliformis, Gigaspora, Glomus, Racocetra, Rhizoglomus and Scutellospora were observed. AMF species diversity and their spore abundance found in M. tenuiflora rhizosphere shown that this native plant species is an important host plant to AMF communities from Brazilian semi-arid region. We concluded that: (a) during the dry period and in semi-arid conditions, there is a high spore production in M. tenuiflora root zone; and (b) soil properties, as soil pH and available phosphorous, affect AMF species diversity, thus constituting key factors for the similarity/dissimilarity of AMF communities in the M. tenuiflora root zone among sites.

Ion-pairing HPLC methods to determine EDTA and DTPA in small molecule and biological pharmaceutical formulations$ / 药物分析学报

Ion-pairing high-performance liquid chromatography–ultraviolet (HPLC–UV) methods were developed to determine two commonly used chelating agents, ethylenediaminetetraacetic acid (EDTA) in Abilifys (a small molecule drug with aripiprazole as the active pharmaceutical ingredient) oral solution and die-thylenetriaminepentaacetic acid (DTPA) in Yervoys (a monoclonal antibody drug with ipilimumab as the active pharmaceutical ingredient) intravenous formulation. Since the analytes, EDTA and DTPA, do not contain chromophores, transition metal ions (Cu2 t , Fe3 t ) which generate highly stable metallocom-plexes with the chelating agents were added into the sample preparation to enhance UV detection. The use of metallocomplexes with ion-pairing chromatography provides the ability to achieve the desired sensitivity and selectivity in the development of the method. Specifically, the sample preparation in-volving metallocomplex formation allowed sensitive UV detection. Copper was utilized for the de-termination of EDTA and iron was utilized for the determination of DTPA. In the case of EDTA, a gradient mobile phase separated the components of the formulation from the analyte. In the method for DTPA, the active drug substance, ipilimumab, was eluted in the void. In addition, the optimization of the concentration of the ion-pairing reagent was discussed as a means of enhancing the retention of the aminopolycarboxylic acids (APCAs) including EDTA and DTPA and the specificity of the method. The analytical method development was designed based on the chromatographic properties of the analytes, the nature of the sample matrix and the intended purpose of the method. Validation data were presented for the two methods. Finally, both methods were successfully utilized in determining the fate of the chelates.

Characterization of the acetohydroxyacid synthase multigene family in the tetraploide plant Chenopodium quinoa

Background Currently, the technology called Clearfield® is used in the development of crops resistant to herbicides that inhibit the enzyme acetohydroxy acid synthase (AHAS, EC 2.2.1.6). AHAS is the first enzyme of the biosynthetic pathway that produces the branched-chain of the essential amino acids valine, leucine, and isoleucine. Therefore, multiple copies of the AHAS gene might be of interest for breeding programs targeting herbicide resistance. In this work, the characterization of the AHAS gene was accomplished for the Chenopodium quinoa Regalona-Baer cultivar. Cloning, sequencing, and Southern blotting were conducted to determine the number of gene copies. Results The presence of multiple copies of the AHAS gene as has been shown previously in several other species is described. Six copies of the AHAS gene were confirmed with Southern blot analyses. CqHAS1 and CqAHAS2 variants showed the highest homology with AHAS mRNA sequences found in the NR Database. A third copy, CqAHAS3, shared similar fragments with both CqAHAS1 and CqAHAS2, suggesting duplication through homeologous chromosomes pairing. Conclusions The presence of multiple copies of the gene AHAS shows that gene duplication is a common feature in polyploid species during evolution. In addition, to our knowledge, this is the first report of the interaction of sub-genomes in quinoa.

Progress of ion-pairing reverse liquid chromatography for analysis of oligonucleotides / 中国生化药物杂志

Therapeutic oligonucleotides have been one of the hottest areas in R&D of drugs in recent years.The research of analysis method has been widely carried out.Ion-pairing reverse liquid chromatography is the most commonly used method for good resolution and easy docking with MS.A review is presented here on the progress of oligonucleotides analysis with ion-pairing reverse liquid chromatography.The column, mobile phase, MS detection, mechanism of analysis will be discussed.

"Research on the implementation and appropriate model of the ""10, 000 primary care doctors to rural households"" campaign in Jining city" / 中华医院管理杂志

Horn-Meter model was used to analyze the problems and obstacles in the10,000 primary care doctors to rural households campaign in Jining city,in terms of policy objectives sand standards,policy resources,institutional characteristics,execution mode,policy implementation environment,and preferences of those who carry out the policy.Based on the energy level and person-post matching,a policy sustainable development model was built and demonstrated,for policy recommendations of optimization and promotion.

Comparison of electrolyte levels between different peripheral blood samples / 国际检验医学杂志

Objective To study the difference of electrolyte levels between different peripheral blood samples .Methods A total of 100 patients were enrolled and collected for artery whole blood samples and vein plasma samples .Potassium ,sodium and chloride levels of artery whole blood ,artery plasma and vein plasma were tested by using ROCHE AVL 9180 analyzer .Results Sodium and chloride levels in artery whole blood and artery plasma were statistically different (P0 .05) .Sodium and chloride levels in artery whole blood and vein plasma were statistically different (P0 .05) .Conclusion Sodium and chloride concentration of ar‐terial whole blood and arterial plasma ,vein plasma could be different ,but the concentration of potassium could be reference for each other .

The Medicine Experience of Commonly Used Paired Drugs and The Three Drugs Pairing of Mr. Chen Zugao / 浙江中医药大学学报

Objective]Aimed to inherit Mr Chen Zugao's clinical experience and understand his medical characteristics. [Methods]Through the review of the herbal medicine books, summarized the experience of commonly used drugs(paired drugs and the three drugs pairing) of Mr. Chen. [Results] Completed the summary about Mr Chen's 8 groups of paired drugs(dui yao) and the three drugs pairing(jiaoyao): guizhi, digupi; guizhi, gansui;chuanshanjia, zaojiaoci; baijiezi, jiuxiangchong; huangqi, tianhuafen; qinjiu, baijili; fuzi, xixin, shigao; huangqi, zhimu, taizishen. [Conclusions]The paired drugs(dui yao) and the three drugs pairing(jiao yao) are the main medicine prescription drugs. A lot of applications wil be done in order to achieve good results. Inferiors wil be harvested who can have careful study of Mr. Chen's experience.

Pharmacokinetics of cefotetan for injection in healthy volunteers / 中国药学杂志

OBJECTIVE: To investigate the pharmacokinetics of cefotetan for injection in Chinese healthy volunteers.

Aprendizagem rápida de comportamento de ouvinte para um bebê / Rapid listener behavior learning by an infant

A exposição dos bebês à apresentação simultânea de um estímulo auditivo e um estímulo visual (pareamento estímulo visual-estímulo auditivo/PVA) ocorre no ambiente natural de interação verbal adulto-bebê. O objetivo deste experimento foi investigar a combinação dos procedimentos de PVA (fotografia-nome) e de matching-to-sample/MTS para a aprendizagem de comportamento de ouvinte por um bebê de 17 meses. A participante foi exposta ao PVA (fotografia-nome) e aprendeu doze relações nome-fotografia em tarefas de MTS. Pseudonomes de duas sílabas foram atribuídos a cada uma de doze fotografias de animais, organizadas em quatro conjuntos. Houve redução progressiva da quantidade de sessões de ensino para cada conjunto, indicando a efetividade da combinação dos dois procedimentos para a aprendizagem rápida de comportamento de ouvinte

Stimulus-stimulus pairing procedures/SSPP occur in natural verbal interaction between adults and babies. The goal of this experiment was to verify listener behavior learning by a 17-months-old infant with a combined procedure of matching-to-sample/MTS and SSPP. The participant learned twelve name-photo relations in MTS tasks, after previous exposition to the SSPP (photo - name). Experimental stimuli were twelve photos of animals and twelve two - syllable pseudo - names which were divided in four sets. The combination of the two procedures was effective for listener behavior learning which occurred with less training sessions fo reach one of the four stimul i set s.

Efeitos do pareamento no perfil de expressão gênica do parasita Schistosoma mansoni / Effects of pairing on the gene expression profiles of the parasite Schistosoma mansoni

Esquistossomose é uma doença crônica e debilitante. Schistosoma representa a única classe de trematódeos com vida dióica. Um contínuo pareamento com o macho é essencial para a maturação sexual do sexo feminino. Fêmeas adultas provenientes de infecções uni-sexuadas são subdesenvolvidas, apresentam atrofia do tamanho e um sistema reprodutivo imaturo. Para estudar os mecanismos envolvidos no pareamento de vermes adultos foram utilizadas duas plataformas de microarranjos distintas: uma composta por 4 mil sondas de cDNA dupla fita produzida pelo nosso grupo de pesquisas e outra composta por 44 mil sondas de oligonucleotideos desenhadas pelo nosso grupo e produzida pela empresa Agilent Technologies. Com a plataforma de 4 mil sondas detectamos 113 transcritos diferencialmente expressos em fêmeas adultas mantidas separadas de seus respectivos pares durante 24 horas de cultivo in vitro quando comparadas com fêmeas adultas pareadas; para 10 destes genes obtivemos uma confirmação adicional da expressão diferencial por transcrição reversa fita específica seguida de PCR em Tempo Real. Observamos também os efeitos do pareamento no perfil de expressão gênica de machos adultos mantidos separados de seus respectivos pares durante 24 horas de cultivo in vitro; foram encontrados 152 transcritos diferencialmente expressos. Com a plataforma de 44 mil sondas foi detectada a expressão de 5.798 genes transcricionalmente ativos em verme adulto, em um conjunto de 19.907 genes únicos representados nesta plataforma. A análise do conjunto de genes "no match" mostrou que em 156 genes ocorria expressão senso e anti-senso; para 6 destes transcritos obtivemos uma confirmação adicional da expressão nas duas fitas por transcrição reversa fita específica seguida de PCR em Tempo Real. Adicionalmente foram identificados 2717 transcritos diferencialmente expressos em fêmeas separadas de seus respectivos pares durante 13 dias de cultivo in vitro, quando comparadas com fêmeas mantidas pareadas...

Schistosomiasis is a chronic and debilitating disease. Schistosoma represents the only class of trematodes with a dioecious life. A continuous pairing with the male is essential for female sexual maturation. Adult females from uni-sexual infections are underdeveloped, have body atrophy and an immature reproductive system. To study the mechanisms involved in pairing of adult worms two microarray platforms were used: one comprised by 4000 cDNA probes and printed by our research group and another comprised by 44 000 oligonucleotide probes designed by our group and printed by Agilent Technologies Company. With the 4000-probes platform we detected 113 transcripts differentially expressed in adult females kept separated from their mates during 24 hours in vitro when compared with paired adult females; for 10 of these genes we obtained additional confirmation of differential expression by Real Time RT-PCR. We also observed the effects of pairing on the gene expression profile of adult males kept separate from their mates during 24 hours in vitro, where we found 152 differentially expressed transcripts. With the 44 000-probes platform we detected the expression of 5798 genes in adult worms, out of a set of 19 907 unique genes represented on this platform. Analysis of the "no match" genes showed that 156 have transcription from the sense and anti-sense strands; for 6 of them we obtained additional confirmation of expression by strand specific Real Time RT-PCR. Additionally, we identified 2717 differentially expressed transcripts in females separated from their mates during 13 days in vitro when compared to females that remained paired. In the analysis of males separated for 13 days we found 243 differentially expressed transcripts. Finally, we performed a study aimed at observing genes which might be correlated to physical contact pairing (male and female) and compared to genes that might be regulated by the possible diffusion of secreted proteins and hormones in...

Efeitos do pareamento no perfil de expressão gênica do parasita Schistosoma mansoni / Effects of pairing on the gene expression profiles of the parasite Schistosoma mansoni

Esquistossomose é uma doença crônica e debilitante. Schistosoma representa a única classe de trematódeos com vida dióica. Um contínuo pareamento com o macho é essencial para a maturação sexual do sexo feminino. Fêmeas adultas provenientes de infecções uni-sexuadas são subdesenvolvidas, apresentam atrofia do tamanho e um sistema reprodutivo imaturo. Para estudar os mecanismos envolvidos no pareamento de vermes adultos foram utilizadas duas plataformas de microarranjos distintas: uma composta por 4 mil sondas de cDNA dupla fita produzida pelo nosso grupo de pesquisas e outra composta por 44 mil sondas de oligonucleotideos desenhadas pelo nosso grupo e produzida pela empresa Agilent Technologies. Com a plataforma de 4 mil sondas detectamos 113 transcritos diferencialmente expressos em fêmeas adultas mantidas separadas de seus respectivos pares durante 24 horas de cultivo in vitro quando comparadas com fêmeas adultas pareadas; para 10 destes genes obtivemos uma confirmação adicional da expressão diferencial por transcrição reversa fita específica seguida de PCR em Tempo Real. Observamos também os efeitos do pareamento no perfil de expressão gênica de machos adultos mantidos separados de seus respectivos pares durante 24 horas de cultivo in vitro; foram encontrados 152 transcritos diferencialmente expressos. Com a plataforma de 44 mil sondas foi detectada a expressão de 5.798 genes transcricionalmente ativos em verme adulto, em um conjunto de 19.907 genes únicos representados nesta plataforma. A análise do conjunto de genes "no match" mostrou que em 156 genes ocorria expressão senso e anti-senso; para 6 destes transcritos obtivemos uma confirmação adicional da expressão nas duas fitas por transcrição reversa fita específica seguida de PCR em Tempo Real. Adicionalmente foram identificados 2717 transcritos diferencialmente expressos em fêmeas separadas de seus respectivos pares durante 13 dias de cultivo in vitro, quando comparadas com fêmeas mantidas pareadas. Para as análises com machos separados durante 13 dias foram encontrados 243 transcritos diferencialmente expressos. Por fim, realizamos estudos com o objetivo de observar os genes que podem estar correlacionados com o contato físico do pareamento (macho e fêmea) e genes que podem ser regulados pela possível difusão de proteínas e hormônios secretados no meio, para os quais a mudança do nível de expressão não dependa da necessidade de contato entre o macho e a fêmea. Sabe-se que o contato direto da fêmea com o macho é necessário para manter a atividade reprodutiva feminina e observamos que o re-pareamento pode restabelecer o perfil de expressão gênica de fêmeas ou machos separados. Além disso, observamos que fêmeas separadas e depois mantidas na presença do macho, porém sem re-pareamento, apresentam uma expressão gênica diferente das fêmeas separadas e depois mantidas na ausência de machos, sugerindo que algum fator secretado pelo macho no meio regula a expressão. Este trabalho representa uma importante contribuição no entendimento da relação macho-fêmea em nível molecular

Schistosomiasis is a chronic and debilitating disease. Schistosoma represents the only class of trematodes with a dioecious life. A continuous pairing with the male is essential for female sexual maturation. Adult females from uni-sexual infections are underdeveloped, have body atrophy and an immature reproductive system. To study the mechanisms involved in pairing of adult worms two microarray platforms were used: one comprised by 4000 cDNA probes and printed by our research group and another comprised by 44 000 oligonucleotide probes designed by our group and printed by Agilent Technologies Company. With the 4000-probes platform we detected 113 transcripts differentially expressed in adult females kept separated from their mates during 24 hours in vitro when compared with paired adult females; for 10 of these genes we obtained additional confirmation of differential expression by Real Time RT-PCR. We also observed the effects of pairing on the gene expression profile of adult males kept separate from their mates during 24 hours in vitro, where we found 152 differentially expressed transcripts. With the 44 000-probes platform we detected the expression of 5798 genes in adult worms, out of a set of 19 907 unique genes represented on this platform. Analysis of the "no match" genes showed that 156 have transcription from the sense and anti-sense strands; for 6 of them we obtained additional confirmation of expression by strand specific Real Time RT-PCR. Additionally, we identified 2717 differentially expressed transcripts in females separated from their mates during 13 days in vitro when compared to females that remained paired. In the analysis of males separated for 13 days we found 243 differentially expressed transcripts. Finally, we performed a study aimed at observing genes which might be correlated to physical contact pairing (male and female) and compared to genes that might be regulated by the possible diffusion of secreted proteins and hormones in the medium, for which the change of expression level does not depend on physical contact between male and female. It is known that direct female-male contact is needed to keep the female reproductively activity and we observed that repairing can restore the gene expression profile of females or males that were kept separated. Furthermore, we observed that females separated and then maintained in the presence of male, but without re-pairing, have a different gene expression from the separated females kept without males, suggesting that some male secreted factors might be involved in gene regulation. This work represents an important contribution to the understanding of male-female relation at the molecular level

Zhang Farong’s Experience in Treating Diabetes / 浙江中医药大学学报

Pro. Zhang Farong treats diabetes by differentiating signs and pairing drugs, such as mulberry bark and wolfberry bark, epimedium and male cocoon, lycium and mulberry, motherwort and Herba Lycopi. And these usually lead to good curative effect. By Chinese drug pair analysis, it shows the organic combination of correct analysis on disease, defining therapy and agile pairing drugs is of great meaning to such diseases as diabetes.

New light on tautomerism of purines and pyrimidines and its biological and genetic implications.

The tautomerism of the natural 1-substituted pyrimidines and 9-substituted purines found in nucleic acids has been re-examined in the light of new experimental data on various nitrogen heterocycles in solution, in the gas phase and, in part, in low-temperature inert matrices. The results are compared with those obtained by quantum chemical calculations, including improved versions of the latter. Examples are presented of natural nucleosides which exhibit appreciable tautomerism in solution, e.g. formycins A and B, isoguanosine, but are not found in DNA. Illustrations are given of synthetic promutagenic nucleosides with pronounced tautomerism in solution relevant to their role in mutagenesis, such as the N4-hydroxy- and N4-methoxy cytidines. The amino-imino tautomeric equilibria of the promutagenic N6-hydroxy- and N6 -methoxy-adenosines are highly dependent on the solvent medium, the proportion of the imino species varying from 10% in CCl4 to 90% in aqueous medium. The type of base pairing of these is dependent on the conformation of the exocyclic hydroxy or methoxy groups. At the monomer level, addition of a potentially complementary base leads to a shift in the tautomeric equilibrium in favour of the species which pairs with this base. Biological and genetical implications of the foregoing are described.

STUDIS ON STRUCTURE KARYOTYPE AND BEHAVIOR OF THE SYNAPTONEMAL COMPLEX IN SPERMATOCYTES OF HEDGEHOG ERINACEUS EUROPAEUS IN SPER DEALBATUS INSECTIVORA / 解剖学报

The structure, karyotype and behavior of synaptonemal complex (SC) in the spermatocytes of hedgehog (Erinaceus euroaaus dealbatus (insectivora)) were analyzed by light and electron microscopy with a modified surface spread preparation which involves the use of sodium dodecyl-sulphate (SDS) and silver staining. The lateral elements of the synaptonemal complex have a constant width of about 50 nm, the distance between the two lateral elements is about 100 nm. The relative length and arm rationale constant for each autosomal SC. The relative length and arm ratio constant for ea of the autosomal SCs are very similar to that of mitotic autosome.The X and Y chromosome axes have a clear morphological distinction from the autosomal SC. The axes of X and Y chromosome pair and form a SC of certain length at pachytene stage. The axes of unpaired X and Y chromosome are heteropycnotic and display various morphological complexities. But these differentiations in hedgehog are primary than in other mammalians, such as human and mice. At pachytene stage the X and Y chromosome display an extensive side-by-side pairing segment with decreasing length as meiotic prophase progressed.