ABSTRACT
AIM To determine the contents of five marker components and total flavonoids in Paishi Granules (a medication to remove stones in the body,containing Glechomae Herba,Akebiae Caulis,Cynanchi Paniculati Radix et Rhizoma,etc.).METHODS The content determination of various components in 50％ methanol extract of Paishi Granules was accomplished by RP-HPLC.The analysis was performed on a 30 ℃ thermostatic Aglient SBC1s column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 240 nm.The content of total flavonoids was detected by ultraviolet spectrophotometry.RESULTS Chlorogenic acid,loganin,rutin,rosmarinic acid and ammoninm glycyrrhizinate showed good linear relationships within the ranges of 18.88-604.00,18.50-592.00,20.25-648.00,18.75-600.00 and 18.81-602.00 μg/mL,whose average recoveries were 97.9％,100.7％,103.3％,96.4％ and 102.4％ with theRSDsof1.4％,0.4％,2.7％,1.2％ and 2.3％,respectively.The contents of total flavonoids in twenty batches of samples all met pharmacopeia requirements,while those of marker components were found to be different,among which loganin displayed the most significant change,followed by rutin and ammoninm glycyrrhizinate.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Paishi Granules.
ABSTRACT
OBJECTIVE:To optimize the extraction process of paishi granule.METHODS:The extraction process of paishi granule was optimized by L 9 (3 4 )orthogonal test with total flavonoids as index,and with duration of decoction,times of de?coction,the amount of water added and the amount of alcohol used for extraction as factors of investigation.RESULTS:The optimum extraction condition was the following,the amount of water added to which was12times that of the medical material,and it was decocted120min for3times,which then was dried and crushed,then60%alcohol,which was40times that of the amount of medicinal material,was extracted by refluxing for3times with30min each time,filtered while hot,and the alcohol was recovered.The yield of extractum and the content of the total flavonoids were fairly high,and there was a good repro?ducibility.CONCLUSION:The optimized process is stable and feasible.