ABSTRACT
Objective Traditional raw pangolin products are not used as medicine, which can only be used as medicine after processing. Therefore, the processing mechanism of high temperature sand-fried pangolin was studied. Methods The changes of liposolubility and protein composition of pangolin before and after processing were analyzed by TLC and Nano LC-Q Exactive Orbitrap MS. Meanwhile, the simulation processing of cyclic dipeptides, which were significantly increased during processing, was performed. The activity of L-serine-L-tyrosine cyclic dipeptide was screened. Results The results showed that there was no significant change in fat-soluble components, significant decrease in polypeptides and significant increase in cyclic dipeptides after the sand-fried processing of pangolin. The formation of cyclic dipeptides was mainly related to the heating of the processing. At low temperature, the N-terminal of the linear peptide could be cycled to form L-shaped cyclic dipeptides. At high temperature, the N-terminal and C-terminal of the linear peptide could be rapidly cycled to form cyclic dipeptides. L-serine-L-tyrosine cyclic dipeptide could prolong coagulation time and increase the proliferation rate of mammary epithelial cells and the expression of genes related to milk protein synthesis in dairy cows. It also had significant analgesic activity, which was consistent with the traditional efficacy of pangolin. Conclusion These results suggested that large amounts of L-serine-L-tyrosine cyclic dipeptide produced by the processing of pangolin may be one of the material bases for enhancing the processing efficiency of pangolin. It was of great significance for revealing the material basis of pharmacodynamics of pangolin, searching for alternative resources and protecting pangolin.
ABSTRACT
Objective:To distinguish Pangolin scales from its adulterants to prevent the use of poor Armadillo scales. Methods:The properties of characters, color, size and so on between Pangolin scales and Armadillo scales were compared. Results:The length and width of genuine Pangolin scales was within the range of 0. 75-5. 50 cm, the color was golden yellow or yellowish brown, the tex-ture was crisp with easily broken property, and the thickness of section was 0. 2-0. 4 cm. The length and width of poor Armadillo scales was 6-10 cm, which was 3-5 times of that of Pangolin scales, the color was yellowish brown or brown, the texture was not crisp with easily broken property, and the thickness of section was 0. 6-1 cm. Conclusion:The above characteristics can be used to discern Ar-madillo scales.