ABSTRACT
Paragonimus harinasutai metacercariae were found in a species of freshwater crab, Indochinamon ou, collected in a small stream of Namback District, Luang Prabang Province, Lao PDR. Adult flukes were recovered after experimental infection of the metacercariae to dogs. Metacercariae were round or slightly elliptical, 0.666 x 0.626 mm in average size, and had a thin cyst wall of about 20 microm in thickness, a black excretory bladder, convoluted ceca, and some pinkish materials in the body. Adults were somewhat elongated, 95.2 x 36.5 mm in average size, covered with single-tipped tegumental spines, had a smaller oral sucker than the ventral sucker, a moderately branched ovary, and 5-6 lobulated testes. Eggs were ovoid and bilaterally symmetrical in shape, 79 x 45 microm in average size, and had a uniformly thickened shell. By the present study, it has been confirmed that I. ou is a new second intermediate host for P. harinasutai.
Subject(s)
Animals , Dogs , Female , Humans , Male , Crustacea/parasitology , Disease Reservoirs/parasitology , Host-Parasite Interactions , Laos , Paragonimiasis/parasitology , Paragonimus/anatomy & histology , Shellfish/parasitologyABSTRACT
Objective To identify Paragonimus harinasutai from Ninghai,Zhejiang Province,China.Methods Meta-cercariae were collected from the crabs Sinopotamon chekiangenes in Xixi village of Ninghai County for ITS2 sequence analysis,CO1 sequence analysis and endonuclease BsaHI and StuI analysis by PCR-RFLP.Results The fingerprintings of PCR-RFLP were virtually same to the isolate from Thailand(Nakorn-nayok).The ITS2 sequence with 366 bp and CO1 sequence with 390 bp of the metacercariae collected from Ninghai revealed a nucleotide identity 95.6% and 89.5% respectively to the Thai isolate.Conclusion The study confirmed that Paragonimus harinasutai is present in Ninghai,China,with certain variation on molecular biology in comparison to the Thai isolate.