Regulation and Function of the Peg3 Imprinted Domain

A subset of mammalian genes differ functionally between two alleles due to genomic imprinting, and seven such genes (Peg3, Usp29, APeg3, Zfp264, Zim1, Zim2, Zim3) are localized within the 500-kb genomic interval of the human and mouse genomes, constituting the Peg3 imprinted domain. This Peg3 domain shares several features with the other imprinted domains, including an evolutionarily conserved domain structure, along with transcriptional co-regulation through shared cis regulatory elements, as well as functional roles in controlling fetal growth rates and maternal-caring behaviors. The Peg3 domain also displays some unique features, including YY1-mediated regulation of transcription and imprinting; conversion and adaptation of several protein-coding members as ncRNA genes during evolution; and its close connection to human cancers through the potential tumor suppressor functions of Peg3 and Usp29. In this review, we summarize and discuss these features of the Peg3 domain.

The study for expression of PEG-3 in rats’ cardiac muscle after electrical injury by the method of immunohistochemistry / 重庆医科大学学报

Objective:To study the expression of PEG-3 in cardiac muscle after electrical injury in order to find the time dependablity and the discrimination for the antemortem electrified and postmotorm electrified.Metheds:The animals were devided into two groups:the negative group and the experimental group.The experimental group were devided into three groups again:the electrical injury group,the electrouction group and the group of electrical shock after death.The electrical injury group is for 0h、3h、6h、12h、24h、3d、6d、12d,the electrouction group is for 0h、1h、3h、6h、12h,and the group of electrical shock after death is for 0h、0.5h、1h、3h,the control group is for 0h、0.5h、1h、3h、6h、12h.After all these we should get the cardiac tissue at the propotional time,and then dye it by the method of HE and immunohistochemistry.Then we can gain the conclusion through the method of semiquantitative analysis.Results:The expression of PEG-3 increased in heart injury early,it may be a useful molecular index to diagnose heart injury induced by electric force.In additional,the rule of PEG-3 expression was changed following time,which may help to estimate the injury interval in forensic EI field.We can also discriminate the the electrouction group and the postmotorm electrified group,because there are only extremely tenuous positive staining for the group of electrical shock after death.Conclusion:PEG-3 can diagnose the electrical injury and discriminate the electrical injury,weather it formed at antemortem or postmortem.