ABSTRACT
Objective: To investigate the chemical constituents of the whole plants of Artemisia rupestris. Methods: The chemical constituents were isolated and purified by silica gel, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the isolated compounds were identified by NMR and MS spectroscopic method. Results: Eighteen compounds were isolated and purified, which structures were identified as 2α-(2',4'-hexadiynoyl)-1,6-dioxaspiro [4,5]-deca-3-ene (1), chrysophanol (2), ethyl vanillate (3), methyl linolenate (4), 8,11-octadecadienoic acid methyl ester (5), eicosane coumaric acid ester (6), emodin (7), 2,5-di-tert-butylphenol (8), phellopterin (9), syringic acid (10), 7-methoxycoumarin (11), vanillin (12), imperatorin (13), oroxylin A (14), 5-hydroxy-3,4',6,7-tetramethoxyflavone (15), erucylamide (16), octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoate (17) and 3,5,3',4'-tetrahydroxy-6,7-dimethoxyflavone (18). Conclusion: Compounds 1, 4, 11-14 and 18 are isolated from A. rupestris for the first time, and all the other compounds were isolated from the genus Artemisia for the first time.
ABSTRACT
Objective: To study the chemical constituents of ethyl acetate soluble parts in 70% ethanol aqueous extract from the roots of Angelica dahurica var. formosana. Methods: The compounds were separated and purified by repeated column chromatography on silica gel and HPLC, and their structures were determined by spectroscopic data analyses of MS and NMR. Results: Forty-two compounds were obtained and identified as imperatorin (1), phellopterin (2), bergaptol (3), heraclenol (4), falcarindiol (5), isoimperatorin (6), byakangelicin (7), psoralen (8), bergapten (9), isogosferol (10), japoangelol A (11), atractylenolactam (12), columbianadin (13), 5-hydroxy-8-methoxypsoralen (14), (8E)-1,8-heptadecadiene-4,6-diyne-3,10-diol (15), pabulenol (16), byakangelicin ethoxide (17), isobyakangelicol (18), umbelliferone (19), xanthotoxol (20), osthole (21), alloimperatorin (22), 5-(2- acetoxy-3-hydroxy-3-methylbutoxy) psoralen (23), oxypeucedanin hydrate (24), oxypeucedanin ethanolate (25), alloisoimperatorin (26), pabularinone (27), oxyalloimperatorin (28), isooxypeucedanin (29), adenine (30), trans-ferulic acid (31), vanillic acid (32), 2-ethoxy-2-(4-hydroxyphenyl)-ethanol (33), suberosin (34), isopimpinellin (35), xanthotoxin (36), ulopterol (37), 2,4- dihydroxybenzoic acid methylate (38), scoparone (39), swietenol (40), marmesin (41), and 5-hydroxymethylfurfural (42), respectively. Conclusion: Except for the compounds 1, 3, 6, 7, 10, 13, 14, 16, 21, 24, and 26, other compounds were isolated from the roots of A. dahurica var. formosana for the first time.