ABSTRACT
AIM:To investigate the ameliorative effect of ischemic postconditioning (I-postC) on pia mater microcirculation in rats subjected to cerebral ischemia reperfusion (I/R) and its mechanisms. METHODS:Thirty-two male Wistar rats were randomly divided into sham,I/R,I-postC,and ischemic preconditioning (IPC) groups. The global cerebral I/R injury was induced by shunting carotid artery in rats. Pia mater microcirculation and cerebral microcirculatory perfusion were measured after reperfusion. The content of soluble intercellular adhesion molecule-1 (sICAM-1) in plasma was detected using enzyme linked-immunosorbent assay (ELISA). Myeloperoxidase (MPO),malondialdehyde (MDA),and superoxide dismutase (SOD) in cerebral tissue were detected. The expressions of vascular endothelial cell cadherin (VE-cadherin) and NF-?B p65 in cerebral tissue were assayed by Western blotting. RESULTS:(1) The disturbance of the blood flow in microvessel induced by I/R was improved significantly by I-postC. In addition,I-postC alleviated significantly the decrease in diameters of microvesseles,cerebral microcirculatory perfusion and cerebral VE-cadherin content induced by I/R (P
ABSTRACT
The animal model of the bulbar conjunctival and pral microcirculation disturbance induced by high molecular weight dextran in rabbit were reported In this experiment.The interre'ationship between the bulbar conjunctival microcired ation disturbance and the pial miciocirculation disturbance, has been observed. We found that the occurrence of the buibar conjunctival microcirculation was earlier than the pial microcirculation. The degrees of abnormal bulbar conjunctival micro-circulation were more prominent. than that of the pial one, however, the degrees of erythrocyte aggregation in the bulbar conjunctiva Was similar to that of the cerebral microcirculation. The experiment showed the bulbar conjunctival microciculation disturbance, to some degree may reflect the degree of the cerebral microcirulation disturbance in living animals.