Effects of glycosaminoglycan extracted from Pinctada martensii on osteoblast proliferation , differentiation and mineralization in vitro / 中国海洋药物

Objective To study the effects of glycosaminoglycan extracted from Pinctada martensii on the proliferation, differentiation and mineralization of newborn rat calvarium osteoblast in vitro. Methods MTT, PNPP and ARS methods were used to measure the cell proliferation, activity of ALP and the function of mineral nodes formation of osteoblasts cultured in vitro. Results Glycosaminoglycan at concentration of 0.008～0.5g?L -1 inhibited mildly the proliferation of osteoblast cells, however, this range of concentrations of glycosaminoglycan markedly increased the ALP activity and stimulated mineral node bone formation in osteoblast. Conclusion Glycosaminoglycan extracted from the Pinctada martensii showed stimulating effects on the differentiation and mineralization of newborn rat calvarium osteoblast in vitro, but the stimulating effect on cells proliferation was not observed.

Preliminary study on anti-fatigue effects of enzymatic hydrolyzed animal protein extracted from Pinctada martensii / 中国海洋药物

Objective To study the anti-fatigue effects of enzymatic hydrolyzed protein from Pinctada martensii.Methods Swimming time,contents of liver glycogen and serum urea nitrogen after swimming were determined.Results Three dosages of EPA were all able to prolong swimming time and decrease liver glycogen consumption and serum urea nitrogen content after exhausted swimming in various degree.Conclusion The EAP has evident anti-fatigue effects.

PRELIMINARY STUDIES ON THE EXTRACTION AND THE ANTITUMOR ACTIVITY OF RIBONUCLEIC ACID ( RNA ) FROM PINCTADA MARTENSII AND PINNA PECTINATA / 中国海洋药物

Ribonucleic acid (RNA) was extracted from Pinctada martensii and Pinna pectinata at a weak alkaline environment by removing the protein and fat through adding phenol and chloroform. It also reported the antitumor activity of RNA on mice with sarcoma-180 and has discovered that RNA has an obvious effect of inhibiting the tumor growth in mice with the inhibition rates being ranged from 41. 2% to 45. l%,and 38. 8% , respectively.

PRELIMINARY EVALUATION OF CYTOCOMPATIBILITY OF PEARLS OF PINCTADA MARTENSII / 中国海洋药物

This paper reports the results of cytotoxicity test of pearls of Pinctada martensii in vitro. The results of relative growth rate (RGR) and observation of cell morphology by putting up extracted solution of pearl powder in culture flask of L929 cell show that pearls of Pinctada martinsii are cytocompatible. The RGR of pearls of Pinctada martensti is lower than that of hy-droxyapatite.