ABSTRACT
Objective:To analyze and compare the volatile oil components of pine needle of Pinus yunnanensis from different habitats. Method:The volatile oils of pine needle of P. yunnanensis from 12 different habitats were extracted by supercritical CO2 extraction （SFE-CO2），the components were analyzed by gas chromatography-mass spectrometry （GC-MS），and relative percentage of each component was determined by peak area normalization method，and SPSS 20.0 software was used for principal component analysis. Result:The 137 compounds were isolated from the volatile oil of pine needle of P. yunnanensis from 12 different producing areas，with 1 sample identified 57 species，2 samples identified 57 species，3 samples identified 54 species，4 samples identified 48 species，5 samples to identify 58 species，6 samples identified 60 species，7 samples identified 53 species，8 samples identified 55 species，9 samples identified 56 species，10 samples to identify 58 species，11 samples identified 54 species and 12 samples to identify 50 species，composed of hydrocarbon，acid，alcohol，ester，aldehyde，ketone，phenol，ether 8 compounds，including 11 common composition including alpha pinene，beta pinene，beta caryophyllene，7C，10C，16C-hexadecatrienoic acid and palmitic acid methyl ester，palmitic acid，phthalic acid dibutyl do acid，alcohol，stearic acid，mountain，wood tar acid. Conclusion:In this study，supercritical CO2 extraction （SFE-CO2） was used to extract the volatile oil of pine needle of P. yunnanensis from different habitats. It was found that the volatile oil of pine needle of P. yunnanensis was mainly composed of hydrocarbons，acids，esters and alcohols. And the content and species of volatile oil of pine needle of P. yunnanensis from different producing areas differ greatly which lay a foundation for the further development and utilization of pine needle of P. yunnanensis resources.
ABSTRACT
Objective To study the chemical constituents of Pinus yunnanensis. Methods The whole plant of air-dried P. yunnanensis was extracted with methnol. The methnol extract was suspended in H2O and extracted with petroleum, ethyl acetate, and n-BuOH, successively. The compounds were isolated and purified by column chromatography from the petroleum and ethyl acetate fractions, and identified based on spectral analyses (MS, 1D- and 2D-NMR, IR, UV). Results Fourteen compounds were isolated from P. yunnanensis and characterized as (7R,8R)-7,8-dihydro-3'-hydroxyl-7-(4-hydroxyl-3-methoxyphenyl)-8-hydroxymethyl-1'-benzofuranpropanol- 4-O-(3-O-methyl-α-L-rhamnopyranoside) (1), pinosylvine (2), stigmast-4-en-3-one (3), kaempferol (4), isopimarinol (5), quercetin (6), pinocembrin (7), taxifilin (8), 3-hydroxyl-5-methoxy-stilbene (9), 5-O-β-D-glucopyranosyl-thymoquinol (10), icariside E4 (11), 3,4-dimethoxyphenyl-1-O-β-D-glucopyranoside (12), schizandriside (13), and β-stitosterol (14). Conclusion Compounds 1, 3-7, and 10-13 are obtained from Pinus yunnanensis Lamb. for the first time.
ABSTRACT
Objective To study the effect of Pinus yunnanensis on acute alcoholic liver injury in rats and explore its mechanism. Methods A model of acute alcoholic liver injury in mice was prepared by alcohol. The mice were randomly divided into normal control group, model group, positive control group, Pinus yunnanensis low-, medium-and high-dose groups. Mice in the medicine group were given the corresponding medicine by gavage once a day for 7 days. After the last three hours of intragastric administration, the liver and spleen index, ALT, AST and GSH in serum, SOD, MDA and NO in liver homogenates were measured. Histopathological changes of liver were observed by HE staining. Results Compared with model group, Pinecone of Pinus unnanensis high-, medium- and low-dose groups could significantly reduce the liver index in mice (P0.05). HE staining results showed that, the damage of liver tissue in mice of Pinus yunnanensis was significantly improved compared with the model group. Conclusion Pinus yunnanensis has protective effects on acute alcoholic liver injury in mice.