ABSTRACT
OBJECTIV E To establish the method for evaluating the quality o f Plantago asi atica and fried P. asiatica . METHODS The fingerprints of 15 batches of P. asiatica and 15 batches of fried P. asiatica were established by HPLC. The common peaks were identified with the Similarity Evaluation System for Chromatographic Fingerprinting of TCM （2012 edition）， and similarity evaluation was performed. Analysis of chemical pattern recognition was performed by using SPSS 25.0 and SIMCA-P 14.1 software（cluster analysis ，principal component analysis and orthogonal partial least squares regression analysis ）. The markers which affected the difference in the quality between P. asiatica and fried P. asiatica were screened with variable importance projection（VIP）value greater than 1. RESULTS There were 18 common peaks in the fingerprints of 15 batches of P. asiatica and 13 common peaks in the fingerprints of 15 batches of fried P. asiatica . A total of 8 common peaks were found in both of them. Their similarities were greater than 0.920. Two common peaks were identified as geniposidic acid ，acteoside. The results of cluster analysis showed that when the spacing was 10，the 30 batches of samples could be clustered into three categories ，with S 1-S5 as one，S16-S20 as one ，S6-S15 and S 21-S30 as one . The results of the pri ncipal component analysis showed that the cumulative variance contribution rate of the first two principal components was 82.575％ . The results of the orthogonal partial least squares regression analysis showed that the VIP values of the three common peaks were greater than 1，namely peak E（acteoside）， peak D （geniposidic acid ） and peak G. CONCLUSIONS Established fingerprints are stable ，simple sina.com and rapid. It can be used for the quality evaluation of P. asiatica and fried P. asiatica ，by combining with analysis of chemical pattern recognition. Acteoside ，geniposidic acid and the component represented by peak G may be the markers affecting the difference in quality of P. asiatica and fried P. asiatica .
ABSTRACT
OBJECTIVE：To study the effects of Plantago asiatica polysaccharide on the proliferation ，migration and invasion of breast cancer cells ，and to investigate its mechanism preliminarily. METHODS ：Using human breast cancer cell MDA-MB- 231 as subjects ，MTT method was adopted to detect the effects of different concentrations of P. asiatica polysaccharide（8，16，32，64 mg/L）on the cell proliferation ability ，and survival rate of the cells was calculated. Scratch test and Transwell invasion test were used to detect the effects of different concentrations of P. asiatica polysaccharide（8，16 mg/L）on cell migration ability and invasion ability. Western blot assay was used to detect the expression of epithelial-mesenchymal transition （EMT）-related proteins [matrix metalloproteinase- 2（MMP-2），MMP-9，E-cadherin，N-cadherin，vimentin]. RESULTS ：Results of MTT assay showed that survival rate of the cells in 32，64 mg/L P. asiatica polysaccharide groups were significantly lower than control group （P＜0.05 or P＜0.01），so that 8，16 mg/L，which did not affect the cell survival rate ，were used as the follow-up drug concentrations. Compared with control group ，relative mobility （12，24 h），relative invasion rate and relative expression of MMP- 2，MMP-9， N-cadherin and vimentin protein were decreased significantly in 8，16 mg/L P. asiatica polysaccharide groups （P＜0.05 or P＜ 0.01），while relative expression of E-cadherin protein was increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：P. asiatica polysaccharide can inhibit the proliferation of breast cancer cells MDA-MB- 231，and inhibit the migration and invasion of the cells by regulating the expression of metastasis and EMT-related proteins.
ABSTRACT
A method of complete acid hydrolysis combined with high performance anion exchange chromatography and pulsed amperometric detection was developed for the monosaccharide composition analysis of arabinoxylan from the seeds of Plantago asiatica L. The parameters including hydrolysis methods, acid types, acid concentration, hydrolysis temperature, hydrolysis time and placement time, which would affect the hydrolysis process, were optimized. The results showed that it would have a better hydrolysis effect for polysaccharide from the seeds of Plantago asiatica L. with 2 mol/L H2 SO4 in an atmospheric oil bath at 120℃for 2 hours. However, the placement time for diluted solution of the hydrolyzed polysaccharide should be less than 6 hours. The polysaccharide was mainly composed of Arabinose (8. 89%) and Xylose (41. 52%) and Galacturonic acid (0. 73%). Glcuronic acid (3. 44%) was detected simultaneously, and there were also trace amounts of Galatose and Glucose. The results were reproducible. Other arabinoxylans from Panicummiliaceum L. shell, Avena sativa L. bran and Hordeum vulgare L. were taken for monosaccharide compositions analysis under the optimal hydrolysis conditions and the analysis results were good. This study would provide a good reference for monosaccharides composition analysis of arabinoxylans from various sources.
ABSTRACT
Anticoccidial effects of the Plantago asiatica extract (PAE) were evaluated in chickens following oral infection with Eimeria (E.) tenella. This study was conducted on the 3-day-old chickens (n=30). Those animals were divided with 3 groups; PAE 0.1% treated/infected (n=10), PAE untreated/infected (n=10) and non-infected control (n=10). Chickens were fed a standard diet supplemented with or without PAE for 1 week prior to infection with E. tenella (10,000 sporulated oocysts per chicken). The effects of PAE on E. tenella infection were assessed by two parameters; fecal oocysts shedding and body weights gain. The PAE-fed chickens produced significantly reduced fecal oocysts (P<0.05) when compared to the E. tenella-infected group fed standard diet. Also, PAE-based diet, improved body weight loss caused by E. tenella infection. Our data demonstrated that PAE had remarkable anticoccidial activities against E. tenella. This finding might have implications for the development of anticoccidial drug. This study is the first to demonstrate anticoccidial effect of PAE on Eimeria parasites.
Subject(s)
Animals , Body Weight , Chickens , Diet , Eimeria tenella , Eimeria , Oocysts , Parasites , PlantagoABSTRACT
Objective To investigate the hypoglycemic effects of the ethanol extracts of Plantago asiatica in different diabetes animal models.Methods The different diabetes animal models (adrenaline -induced hyperglyce-mic models,glucose -induced hyperglycemic models,alloxan -induced hyperglycemic models)have been applied. Results Compared with model groups,the ethanol extracts of Plantago asiatica at high dose[(10.26 ±1.76)mmol/L] possessed obviously hypoglycemic effects on adrenaline -induced hyperglycemic mice[(14.74 ±2.47)mmol/L](t =2.558,P <0.01)and the middle dose[(11.74 ±2.35)mmol/L]possessed hypoglycemic effects on adrenaline -induced hyperglycemic mice[(14.74 ±2.47)mmol/L](t =1.524,P <0.05 );Compared with model groups,the ethanol extracts of Plantago asiatica at high dose[(11.38 ±1.76)mmol/L]possessed obviously hypoglycemic effects on glucose -induced hyperglycemic mice[(16.15 ±2.49)mmol/L](t =2.710,P <0.01 )and the middle dose [(13.01 ±3.23)mmol/L]possessed hypoglycemic effects on glucose -induced hyperglycemic mice(t =1.334,P <0.05);In addition,the ethanol extracts of Plantago asiatica could significantly inhibit the rise of blood glucose level and had certain hypoglycemic effect on halloxan -induced diabetic mice.Conclusion The ethanol extracts of Planta-go asiatica have a significant role in lowering blood glucose at a certain extent.
ABSTRACT
This study was aimed to explore the resource diversity and microbial inhibition activity of endophytic fungi from medicinal plant Plantago asiatica L. The endophytic fungi were isolated from the root, stem and leaf of the host by tissue inoculation culture and five plant pathogenic fungi and four bacteria strains used as indicating microbes to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion methods. The results indicated that thirteen fungal endophytic strains were isolated from the host. Most of them came from stem, then leaf, and root as the least in number. The isolated strains attribute to five genera, two fam-ilies, and two orders based on morphological characteristics. For the isolated strains, eleven of them were found to have some microbial inhibition activities against one or more indicating fungi, making up 84.6% of the total iso-lates. Six isolated strains had some antimicrobial activities against one or more indicating bacteria, amounting to 46.2% of the total isolates. Three isolated active strains, which are PAEFS001, PAEFS007 and PAEFS008, ex-hibited evident inhibition activities against five kinds of pathogenic fungi used in the trials respectively. The strain of PAEFS001 ascribed to Ozonium sp. Both strains of PAEFS007 and PAEFS008 ascribed to Aspergillus sp. One active strain of PAEFS003 showed evident antibacterial activities to Bacillus subtilis and Staphyloccus aureus, which belonged to Fusidium sp. The endophytic fungi from medicinal plant Plantago asiatica L. have evident an-timicrobial activities. Their inhibition activities against pathogenic fungi have relatively broad spectrum. And their inhibition activities to both Bacillus subtilis and Staphyloccus aureus as G+ are evident and have certain selectivi-ty. It is feasible to find new bioactive compounds associated with endophytic fungi from Plantago asiatica L. Fur-ther research and development of the endophyic fungi will be important for the integrated utilization of the host.
ABSTRACT
Objective To identify the active compounds for protein tyrosine phosphatase 1B (PTP1B) from the seeds of Plantago asiatica. Methods Bioassay-guided fractionation resulted in the isolation of iridoid glucosides (1-5) with PTP1B inhibitory activity. Results Five compounds were identified as desacetylhookerioside (1), melittoside (2), geniposidic acid (3), 10-O-acetyl-geniposidic acid (4), and alpinoside (5). Conclusion Isolated compounds 3-5 inhibit PTP1B with IC50 values ranged from (16.3 ± 1.1) to (19.8 ± 1.2) μmol/L.
ABSTRACT
Extract F1 from Plantago asiatica L. provided hepatoprotective activity, decreased liver GPT enzyme (91.7%) and serum bilirubine (25.8%) in model of CCl4-induced cirrhosis mice, versus control group. Extract F1 had hepatoprotective activity, decreased liver GPT enzyme and serum bilirubine in model of CCl4-induced cirrhosis mice, versus control group (27.5% and 19.4%, respectively). Extract F1 had anti-oxidant activity, decreased liver MDA level by 21.2%, inhibited cirrhotic process, decreased collagen level by 26.5% versus control group
Subject(s)
Medicine, Traditional , Plants, Medicinal , LiverABSTRACT
OBJECTIVE: To establish an HPCE and polymerase chain reaction (PCR) assay for identification of Descurainia sophia and Plantago asiatica.METHODS: PCR products of D. sophia and P.asiatica were analyzed by capillary tube (60 cm?75 ?m), taking 30 mmol?L-1 sodium tetraborate decahydrate (pH 9.2) as run buffer. The injection sample was performed by high pressure of 5 s at a separation voltage of 12 kV and with UV detection wavelength of 254 nm. RESULTS: PCR products of D. sophia and P. asiatica were separated by HPCE rapidly and accurately. The significant differences were noted in electrophoregram between PCR products of D. sophia and P. asiatica.CONCLUSION: This method is simple, rapid and sensitive for identification of D.sophia and P. asiatica.
ABSTRACT
Objective: To investigate the effect of Plantago asiatica L. on antioxidation in rats. Methods: The antioxidative enzymes and LPO of serum, heart, and liver tissues were determined. Results: The activities of superoxide (SOD) in serum and heart were significantly lower than that of the control. The lipid peroxide (LPO) level in serum and heart was markedly higher than that of the control. Serum and liver catalase (CAT) activities in rats fed with high fat were decreased. Activities of liver glutathione peroxidase (GSH Px) in high fat rats were decreased significantly. The production of SOD activities in serum and liver and GSH Px in liver were increased significantly in rats maintained on Plantago asiatica L. supplemented diets, meanwhile serum and heart LPO were reduced. Conclusion: 15g/kg Plantago asiatica L. can increase the antioxidation against lipid peroxide in hyperlipidemic rats.