Estimation of RBC membrane and serum lipid composition in central Indian sickle cell disease population with and without pulmonary hypertension.

Aim: This study is designed to estimate and examine the relation between the levels of RBC membrane and serum lipids in central Indian sickle cell disease population with and without Pulmonary Hypertension .Methods: This study was carried out on central Indian sickle population at the Dept.of Biochemistry at MGM medical college & M.Y. hospital, Indore. From june 2011 to October 2012. Plasma Lipid concentrations were determined in 135 Sickle cell disease (SCD) patients, out of this 65 patients had sickle cell disease with Pulmonary Hypertension (SCD-PH) and 80 normal healthy matched individuals (controls). Study group comprises of both male and females in the age group of 18-56 years. Weight , height, waist hip ratio and blood pressure were recorded. All the blood samples were analyzed to determine the serum lipid concentration and RBC membrane lipid composition. Results: The body mass index and the systolic blood pressure of SCD with Pulmonary Hypertension (27.87 ± 4.68, 128.60 ± 22.49 mmHg) and without pulmonary hypertension (25.87 ± 4.68, 125.23 ± 15.89 mmHg ) were higher when compared with controls (24.67 ± 5.18, 119.15 ± 13.03 mmHg). The SCD with PH population (1.21 ± 0.07) and SCD without PH (1.09 ± 0.04) subjects showed significantly higher levels of RBC membrane cholesterol compared with controls (0.84 ±0.01). The trends of decreased serum cholesterol and normal high-density lipoprotein(HDL) levels in SCD patients were noted as compared with controls and these levels are statistically significant. The low-density lipoprotein cholesterol (LDL) was also significantly lower in SCD-PH and SCD when compared with control subjects. Interestingly serum Triglyceride levels are highly elevated in SCD-PH (208.43±.56.97) when compared with SCD (132.34±8.97) and controls (141.43±26.98). Elevated TGL concentrations are positively correlated with haemolytic markers (Lactate dehydrogenase and Total Bilirubin, (r=0.326 and r=0.468,P<0.001)). On a prospective screening of SCD population we found that around one third total population are with elevated tricuspid regurgetant jet velocity (TRJV) of 2.5m/s or higher. Conclusion: Our study data suggests that there is a relationship between RBC membrane and serum lipids in SCD population. Significant increase in RBC membrane cholesterol and decreased phospholipids in sickle cell disease play an important role in the fluidity and structural stability of the membrane and possibly in the Hemolysis and sickle shape of RBC. In pulmonary hypertension except the elevated levels of TGL remaining serum lipids are equal to the normal SCD population. This indicates the relation between TGL and vascular dysfunction in SCD.

Efeito de 12 semanas de treinamento com pesos sobre a força muscular, composição corporal e triglicérides em homens sedentários / 12-week resistance training effect on muscular strength, body composition and triglycerides in sedentary men

O objetivo do presente estudo foi verificar o efeito de 12 semanas de treinamento com pesos (TP) sobre a força muscular, composição corporal e triglicérides em homens sedentários. Para tanto, 14 homens saudáveis e sedentários foram separados aleatoriamente, de forma balanceada, em grupo experimental (GE = 29 ± 1 anos; 180,0 ± 3,0cm; 80,5 ± 1,8kg) e grupo controle (GC = 27 ± 1 anos; 170,0 ± 2,0cm; 76,2 ± 0,9kg). O GE foi submetido a um protocolo de TP durante 12 semanas, com frequência de três vezes por semana. O programa de TP foi composto por 10 exercícios executados em duas séries de 10-20 repetições. Medidas de massa corporal, estatura, espessura de dobras cutâneas e triglicérides foram realizadas antes e após o período de intervenção. Adicionalmente, o teste de uma repetição máxima (1RM) foi aplicado nos exercícios supino em banco horizontal e mesa extensora, nos período pré, após seis e 12 semanas de acompanhamento. Foram encontradas diferenças significativas (P < 0,05) após as 12 semanas de TP apenas no GE para o somatório das dobras cutâneas, ao passo que a massa corporal e os triglicérides não sofreram alterações nesse período. Com relação aos valores de força muscular, identificou-se aumento significativo (P < 0,05) no GE em ambos os exercícios entre o primeiro e o segundo teste de 1RM e entre o segundo e o terceiro. Conclui-se, portanto, que 12 semanas de TP foram suficientes para aumentar a força muscular e reduzir o somatório de dobras cutâneas sem, contudo, alterar os valores de triglicérides e massa corporal.

The purpose of this study was to investigate the effect of 12 weeks of resistance training on muscle strength, body composition and triglycerides in sedentary men. Fourteen healthy men were randomly divided into experimental (EG = 29 ± 1 yrs; 180.0 ± 3.0 cm; 80.5 ± 1.8 kg) and control (CG = 27 ± 1 yrs; 170.0 ± 2.0 cm; 76.2 ± 0.9 kg) groups. The EG subjects participated in a resistance training protocol during 12 weeks, three times per week. The program of resistance training included 10 exercises performed in two sets of 10-20 repetitions. Body mass, height, skinfold thickness and triglycerides were measured before and after the 12 weeks of intervention. One maximal repetition test (1RM) was done to measure muscle strength. Significant differences (P<0.05) were observed on the sum of skinfolds only in EG after the resistance training, while body mass and triglycerides did not change in neither groups. Muscle strength increased (P<0.05) in both exercises performed between the first and the second and between the second and the third 1-RM tests. Therefore, 12 weeks of resistance training were enough to improve muscle strength and sum of skinfolds, but without promoting any changes in triglycerides and body mass.

Correçäo da interferência dos triglicérideos na dosagem da hemoglobina e na determinaçäo dos índices hematimétricos / Correction of the interference of the triglycerides in the dosage of hemoglobin and hematimetric indexes

A interferência dos triglicerídeos plasmáticos sobre a determinação da hemoglobina foi observada em dosagens acima de 800 mg/dl, provavelmente devido ao aumento da turbidez. Este efeito espúrio leva a falsos resultados no cálculo da HCM e da CHCM. A substituição do plasma lipêmico por soluçäo salina corrige os resultados superestimados da hemoglobina, bem como permite obter resultados mais exatos no cálculo da HCM e da CHCM. (AU)

Plasma triglycerides interference on spectrophotometric hemoglobin determination wasobserved in blood samples with more than 800mg/dl, probably due to the turbidity increase. This spuriouseffect leads to false results in MCM and MCHC determination. Substituting saline solution for thelipaemic plasma corrects the overestimated hemoglobin determination as well as affording more accurateMCH and MCHC values. (AU)