The Effect of Oral Steroid for the Expression of Tumor Necrosis Factor-alpha (TNF-alpha), Transforming Growth Factor-beta1 (TGF-beta1), Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) in Nasal Polyp / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Glucocorticoids are currently the most potent medication available for the treatment of nasal polyposis, but exact mechanisms are uncertain. The purpose of this study was to evaluate the response of transforming growth factor-beta1 (TGF-beta1), tumor necrosis factor-alpha (TNF-alpha), and platelet-derived endothelial cell growth factor (PD-ECGF) to oral corticosteroid in nasal polyps. MATERIALS AND METHOD: Oral steroid-untreated nasal polyps (n=10) and oral steroid-treated nasal polyps (n=13) (prednisolone 30 mg per day for 7 days) underwent nasal endoscopy and biopsy of the polyps. The mRNA expression of these factors were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of these factors in nasal polyp tissues were evaluated by immunohistochemistry. RESULTS: The TNF-alpha mRNA and PD-ECGF mRNA expressions were significantly decreased in the oral steroid-treated nasal polyps when compared with the oral steroid-untreated nasal polyps, but TGF-beta1 mRNA was not significantly decreased. The immunohistochemical studies revealed that levels of expression for TNF-alpha protein and PD-ECGF protein in the oral steroid-treated nasal polyps were significantly decreased, but TGF-beta1 protein was not decreased when compared with oral steroid-untreated nasal polyps. CONCLUSION: We suggest that oral corticosteroids may exert a beneficial effect by significantly reducing the levels of TNF-alpha and PD-ECGF in nasal polyps.

Expression of Basic Fibroblast Growth Factor(bFGF), Vascular Endothelial Growth Factor(VEGF), and Platelet-Derived Endothelial Cell Growth Factor(PD-ECGF) in Nasal Polyps / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES : The etiology of polyp is still largely unknown. Vascular remodelling, with local angiogenesis and plasma leakage, could play a role in the growth process of nasal polyps. The aim of this study was to detect and localize angiogenesis factor expressions in nasal polyp tissue and compare these findings with those of inferior turbinate tissues. MATERIALS AND METHODS: The author examined specimens from patients undergone elective turbinectomy (n=10) or nasal polypectomy (n=23). The mRNA expressions of angiogenesis factors were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern blot analysis. The protein expression of angiogenesis factors in nasal polyp tissues were evaluated by immunohistochemistry. RESULTS: Semi-quantitative RT-PCR/Southern analysis of RNA demonstrated that the level of mRNA expression of basic fibroblast growth factors (bFGF) and vascular endothelial growth factors (VEGF) were decreased in nasal polyp tissues compared with inferior turbinate specimens. The platelet-derived endothelial cell growth factor (PD-ECGF) mRNA expression was significantly increased in nasal polyps compared with inferior turbinates. The immunohistochemical studies revealed that a immunoreactivity to these angiogenesis facors were localized to epithelium, inflammatory cell, and vascular endothelium in all samples reviewed. The high levels of expression for VEGF protein and PD-ECGF protein in the nasal polyp tissues were demonstrated. CONCLUSION: We suggest that PD-ECGF and VEGF contribute to the growth of nasal polyps. Hypoxia may play an important role in the growth of the nasal polyps considering that VEGF and PD-ECGF, secretions of which are stimulated by hypoxia, were highly expressed in the nasal polyp tissues.

The role of PD-ECGF and DPI in hepatic metastasis of patients with colorectal carcinoma / 解放军医学杂志

Objective To investigate the changes in Doppler perfusion index(DPI)of the liver and the platelet derived-endothelial cell growth factor(PD-ECGF)in patients with liver metastasis from colorectal carcinoma by determination DPI and PD-ECGF mRNA and protein expression levels.Methods One hundred patients were enrolled,including 50colorectal carcinoma patients(22of whom with liver metastasis and 28patients without liver metastasis)and 50healthy subjects as control.For all the objects involved,hepatic perfusion was documented with color Doppler flow imaging,and the DPI(hepatic arterial/total liver blood supply)was computed.The expression levels of protein and mRNA of PD-ECGF in 50specimens of carcinoma and adjacent tissues of colorectal carcinoma were assayed by immunohistochemistry and real-time fluorescence quantitative PCR methods in patients with colorectal carcinoma.Results According to pathology and imaging results,the DPI of colorectal carcinoma patients with liver metastasis was significantly higher than that in healthy control group and non-liver metastasis group(P0.05).According to the results of immunohistochemistry and real-time fluorescence quantitative PCR,the expression levels of PD-ECGF protein and mRNA were significantly higher in colorectal carcinoma than that in adjacent tissues(P

Expression of Vascular Endothelial Growth Factor and Platelet-Derived Endothelial Cell Growth Factor in Head and Neck Squamous Cell Carcinoma and It's Relation to Angiogensis / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Tumor angiogenesis is an essential process required for growth and metastasis in cancer. Vascular endothelial growth Factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) are known to be angiogenetic factors. The objectives of this study were to measure the expression of VEGF, PD-ECGF and microvessel density (MVD) in head and neck squamous cell carcinoma (HNSCC) and compare them to normal larynx. We also evaluated relationships of VEGF, PD-ECGF and MVD to clinicopathologic findings in HNSCC. MATERIAL AND METHODS: The expression of VEGF, PD-ECGF and MVD were evaluated by immunohistochemical staining of 26 cases of HNSCC and 6 cases of normal larynx. RESULTS: The expressions of VEGF, PD-ECGF and MVD in HNSCC were significantly higher than in the normal control (p<0.05). MVD were significantly correlated with VEGF and PD-ECGF expressions in HNSCC (p<0.05). The VEGF, PD-ECGF expression and MVD correlated with many clinicopathologic findings in HNSCC. CONCLUSION: These results suggest that VEGF and PD-ECGF are involved in angiogenesis and are related to clinicopathologic findings of HNSCC. Furthermore, we propose that expressions of VEGF, PD-ECGF and MVD to be investigated more in the future as prognostic indicators of HNSCC.