ABSTRACT
Objective To compare the tannin contents of Platycladus orientalis(L.) Franco and its carbonisatus.Method A tungsten molybdophosphate-casein colorimetric method was used with gallic acid as reference substance.Results The standard curve in the range of 0.026～0.26 mg(r =0.999 4),and the average recovery rate was 97.85%,RSD=1.07%(n =9).Conclusion The method is reliable and can be used to determine the tannic of and its different processed products.
ABSTRACT
Objective To establish an HPLC method for the determination of isopimaric acid in the leaves of Platycladus orientalis and its preparations Cebai No.V Capsula and Isopimaric Acid Capsula to evaluate the quality of them. Methods HPLC Method was developed. Kromasil C18(200 mm?4.6 mm, 5 ?m) column was used and the mobile phase: methyl alcohol-water-acetic acid (60∶37∶3), float rate: 1 mL/min, temperature: 25 ℃, wavelength: 231 nm, injected volume: 10 ?L. Content of isopimaric acid in samples was calculated by external standard. Results The linearity relationship of isopimaric acid was good in the range of 1.25-10.00 ?g. The equation of standard curve: Y=66 214 X+2 487.9, r=1.000. The average recovery was 95.8%, RSD=2.7% (n=5). The determination results of isopimaric acid in three batches of P. orientalis were 3.09, 3.20, and 3.56 mg/g; three batches of Cebai No.V Capsula were 10.39, 9.67, and 10.99 mg/g; three batches of Isopimaric Acid Capsula were 553.89, 541.50, and 528.83 mg/g. Conclusion The method is reliable, simple, and precise, which could be used for the quality control of P. orientalis and its capsules.