ABSTRACT
Objective: To establish a method of the determination of total platycodin and platycodin D; And to determine the contents of total platycodin and platycodin D in Platycodi RadiX from different habitats. Methods: The content of total platycodin was determined by 1ultrasonic extraction and weight method; And the content of platycodin D was determined by HPLC. The chromatographic conditions were Waters Symmetry-C18 column, mobile phrase of CH3CN-H2O (22:78), flow rate of 1.0 mL/min, detection wavelength at 210 nm and column temperature 30℃. Results: There was a greater difference between the contents of total platycodin and platycodin D in Platycodi Radix from different habitats. In measuring Platycodi Radix, the content of total platycodin in Platycodi Radix from Zhejiang Province was the highest, which was 12.03%; the contents in Platycodi Radix from Heilongjiang, Liaoning, Anhui, Shandong, and Jiangxi Provinces were more than 6%; However, the contents in Platycodi Radix from the other provinces were lower than 6%, and it was the lowest in Platycodi Radix from Henan province, which was 1.57%. The contents of platycodin D in Platycodi Radix from Yunnan province was the highest, and it was the lowest in Platycodi Radix from Hebei province. Conclusion: The methods could be used for the determination of total platycodin and platycodin D in Platycodi Radix because of theirs simple operations with accurate and reliable results. The contents of total platycodin and platycodin D are different obviously in Platycodi Radix from different habitats, and the contents of total platycodin and platycodin D in the same drug with the level of inconsistency don't show correlation.
ABSTRACT
Objective: To investigate the effects of different soaking treatments by KNO3, KMnO4, H2O2, GA3, and distilled water for different times on seed germination and seedling growth of Platycodon grandiflorum. Methods: Adopting a double-layer filter paper culture method, the seeds were cultured in the 12 h illumination light incubator at 25 °C, and the germination energy, germination percentage, germination index of the seeds, and the root length and the shoot height of the seedling were recorded. Then the data were analyzed. Results: The best soaking treatment to break seed dormancy, promote the seed germination, and improve the seedling growth of P. grandiflorum was with 0.150 g/L GA3 for 24 h. In addition, another effective soaking treatment is using 0.005 g/mL KNO3 for 12 h. Conclusion: The appropriate soaking reagent and time for the seed germination of P. grandiflorum are obtained, which could provide the guidance for seedling and artificial cultivating P. grandiflorum.
ABSTRACT
Objective To get a new approach to conserve the germplasm of Platycodon grandiflorum. Methods A method of vitrification was studied to cryopreserve in vitro shoot-tips of P. grandiflorum, and the regenerated plantlets were observed subsequently. Results Shoot-tips, 2—3 mm length, gotten from in vitro shoots of P. grandiflorum precultured on MS medium supplemented with 5% DMSO and 103 g/L sucrose for 3 d, were loaded with the 60% PVS_2 for 20 min at 20 ℃, and incubated in PVS_2 for 90 min at 0 ℃ prior to a direct plunging into liquid nitrogen (LN) and keeping for 1 d. After rapid thawing in water at 40 ℃, the shoot-tips were rinsed in the MS medium supplemented with 410 g/L sucrose for 20 min, and plated on the filter paper sustained by the MS regeneration medium supplemented with 0.6 mg/L KT, 0.2 mg/L BA, and 0.05 mg/L NAA for 1 d in dark and subcultured on the above regeneration medium for one week in dark prior to exposure to the light. The survival of shoot-tips was up to 80%, and they grew normally. Conclusion The method of vitrification to cryopreserve the germplasm of P. grandiflorum is simple in handling with high in survival and normal in regeneration and can be applied in practice.