ABSTRACT
Objective: To study the chemical constituents in the pseudobulbs of Pleione bulbocodioides. Methods: The chemical constituents from the EtOAc fraction of ethanol extract from pseudobulbs in P. bulbocodioides were isolated and purified by various chromatographic methods, including silica gel, ODS, and Sephadex LH-20 columns, and semi-preparation HPLC. Structures of the isolated compounds were elucidated based on NMR, and MS data, as well as by comparison with the previous data. Results: Thirteen compounds were obtained from the EtOAc fraction of ethanol extract and identified as 4, 4'-dihydroxy-bisphenxyl (1), phillygenin (2), (+)-epipinoresinol (3), 2, 5, 2', 5'-tetrahydroxy-3-methoxybibenzyl (4), chrysophanol (5), physcion (6), 5, 7-dihydroxy-8- methoxyflavone (7), β-sitosterol (8), batatasin III (9), p-hydroxybenzoic acid (10), shanciol F (11), 3, 3'-dihydroxy-2-(4-hydroxybenzyl)-5-methoxybibenzyl (12), and 3', 5-dihydroxy-2-(4-hydroxybenzyl)-3-methoxybibenzyl (13). Conclusion: Compounds 1-8 are isolated from the plants in genus Pleione D. Don for the first time.