ABSTRACT
An investigation was designed for the phytochemical screening by spectroscopic techniques and to determine the anti-in?ammatory activity of Plumeria rubra L. leaves extract. The qualitative UV-VIS spectrum showed the peaks at 333.15 with absorption 0.0731 respectively. The FT-IR spectrum of the Plumeria rubra L. showed the -1 -1 -1 -1 -1 -1 absorption at 1019.79 /cm-1, 1633.07 cm . 2920.54/ cm , 3416.88/ cm ,481.33/ cm , 700.89/ cm , and 2851.70/ cm , 2926 cm-1, 2855 cm-1, 1609 cm-1. Anti-in?ammatory activity of ethanolic leaf extract of Plumeria rubra L. showed that the percentage of inhibition 54.57% at 500?g/ml concentration was evidently higher than the lower concentration of 50 ?g/ml (8.23%). The presence of various bioactive compounds justi?es the use of the leaves for various ailments by traditional practitioners.
ABSTRACT
Objective: The current study was designed to evaluate the protective effect of standardized hydroalcoholic extract of Plumeria rubra (HAEPR) against cisplatin-induced nephrotoxicity in Wistar rats. Methods: HAEPR was administered orally at 3 dose levels (100,200,400 mg/kg). Vitamin E (250 mg/kg) was used as a Standard nephroprotective agent. The kidney function test (estimation of serum creatinine, albumin, blood urea nitrogen) oxidative stress study (estimation of superoxide dismutase, malondialdehyde activity) and histological examination of kidneys was conducted. Results: The efficacy of HAEPR was compared with Cisplatin (CP) treated group. Serum creatinine and BUN was significantly (p<0.01) elevated in CP-treated group compared to the control group. HAEPR (100,200 mg/kg) and Vitamin E (250 mg/kg) significantly (p<0.01) decreased the serum creatinine and BUN levels. CP treated group exhibited significant (p<0.01) decrease in albumin when compared to control. Significant (p<0.01) increase in the serum albumin level was found in HAEPR (100,200 mg/kg) and Vitamin E (250 mg/kg) compared to CP group. Significant (p<0.01) decrease in the activity of SOD was observed in the CP group as compared to control. HAEPR (100 and 200 mg/kg) and Vitamin E (250 mg/kg) significantly (p<0.01) increased SOD levels. HAEPR (400 mg/kg) significantly (p<0.05) increased SOD levels. HAEPR (100,200,400 mg/kg) significantly (p<0.01) decreased MDA levels as compared to CP group. Histopathological examination of the kidneys showed that HAEPR markedly ameliorated Cisplatin-induced renal tubular necrosis. An extract was found effective at all doses, although low dose (100 mg/kg) was found to be more effective and comparable with the standard group (Vitamin E 250 mg/kg). Conclusion: Present investigation revealed that HAEPR resulted in attenuation of Cisplatin-induced renal damage in rats.
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To provide pharmacological evidence of plumeria rubra as antipyretic agent in practice many parts of Pakistan. Antipyretic effect of methanolic extract of the bark of plumeria rubra was investigated.on yeast , dinitrophenol and E-coli induced model. Intraperitoneal administration of yeast, dinitrophenol and E-coli in albino rabbits leads to pyrexia. Intraperitoneal administration of methanolic extract of the bark of plumeria rubra at a dose 100mg/kg body weight were shown significantly reduce the elevated body temperature of rabbit in yeast induced model which was compared with aspirin (Standard Drug) and solvent used. In dinitrophenol induced pyrexia, mixture of verapamil (2mg/kg) and Plumeria rubra (50mg/kg), while in In E-coli induced pyrexia, mixture of Ciproflaxacin (10mg/kg) and Plumeria rubra (50mg/kg), significantly reduced the elevated temperature in all models as compared to the standard drug Aspirin.
ABSTRACT
Aims: To evaluate the analgesic and anti-inflammatory effects of ethanolic bark extract of Plumeria rubra on experimental animal models. Study Design: Assessment of antinociceptive and anti-inflammatory activity. Place and Duration of Study: Department of Pharmacy, North South University, Dhaka, Bangladesh, between January 2011 and June 2011. Methodology: The analgesic activity was evaluated by hot plate, acetic acid induced writhing and formalin induced writhing method in Swiss Albino mice divided into 4 different groups (control, standard diclofenac sodium and extract at two different doses of 250 and 500 mg/kg BW). The extract was also investigated for the anti-inflammatory effect on Long Evans rats using carrageenan induced rat paw edema method. For antiinflammatory study, 24 rats were divided into 4 different groups each receiving either distilled water, standard drug or the extract at the doses of 250 and 500 mg/kg BW. Results: Phytochemical analysis of the extract revealed the presence of tannins, alkaloids, flavonoids and terpenoids. The extract elicited a highly significant (p<0.001) analgesic activity in a dose dependent manner on hot plate method, acetic acid induced writhing test and also on both the early and late phases of formalin test at the doses employed. In the hot plate method, the extract increased the reaction time of heat sensation to 60.81% and 66.52% at the doses of 250 and 500 mg/kg BW respectively while that of the standard drug was 57.40% at the 3rd hour of study. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 62.87% and 70.66% at 250 and 500 mg/kg doses respectively (p<0.001) which were even better than the standard drug diclofenac sodium (50.30%). The extract also significantly inhibited the licking response at the dose of 500 mg/kg in both the early phase (55.11%, p<0.01) and the late phase (66.43%, p<0.01) of formalin test while the standard drug inhibited by 52.27% and 72.03%, respectively. The oral administration of the extract significantly (p<0.001) inhibited inflammatory response induced by carrageenan in a dose dependent fashion. The most prominent inhibition of 61.68% (250 mg/kg) and 73.65% (500 mg/kg) were observed at the 4th hour of study. Conclusion: The central and peripheral analgesic as well as anti-inflammatory effect of the ethanolic bark extract of P. rubra may be due to the presence of various chemical constituents specially flavonoids, tannins, alkaloids or terpenoids. These experimental findings would further establish the scientific basis of the traditional uses of the plant in the management and/or control of pain as well as inflammatory conditions.
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Endophytic microorganisms are recognized as potential source of novel chemical molecules that might be useful in the treatment of infectious diseases. In this study, six medicinal plants (Morinda citrifolia, Plumeria rubra, Artocarpus altilis, Musa nana, Sansevieria trifasciata and Saccharum officinarum) traditionally. They are used for treatment or management of symptoms such as cough, fever and skin diseases, were investigated for the antimicrobial activity of metabolites produced by endophytic microorganisms. Seven endophytes were isolated using the mycological media, potato dextrose agar (PDA). Three of the endophytes were identified as fungi from the Aspergillus species, three as actinomycetes and one as a gram negative bacterium. All endophytic isolates were subjected to anti-bacterial, anti-tuberculosis (TB), anti-fungal, anti-HIV, and cytotoxicity assays using micro-broth dilution assay technique in sterile 96-well plates. All isolates displayed antimicrobial activity inhibiting at least one of the test pathogens at the concentration of 100μg/ml. Of the 7 endophytic isolates, 6 isolates were active against TB, 7 were active against the bacterial test pathogens, and 2 were active against fungi while none were active against HIV. From the 7 isolates, 3 were non-toxic to mammalian cells at a concentration of 100μg/ml.
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To evaluate the potential abortifacient activity of the aqueous, alcohol, ethyl acetate and chloroform extracts of P. rubra pod in female albino rats 50, 100 and 200 mg/kg body weight doses of each extract were administered from day 11 to 15 of pregnancy and animals were allowed to go full term. The phytochemical screening revealed the presence of alkaloids, flavonoids, simple phenolics, steroids, tannins and saponins. Clinical toxicity symptoms such as respiratory distress, salivation, weight loss, dull eyes, diarrhea, and change in the appearance of fur as well as mortality were not observed in the animals at any period of the experiment. All the four extracts of P. rubra pods exhibited abortifacient activity (8-100%). The extracts significantly reduced the number of live fetuses, whereas the resorption index and post implantation losses increased significantly. The % of abortion was found to be highest (100%) with 200 mg/kg dose of alcoholic extract of P. rubra pods.