ABSTRACT
Objective Mining Paris resources with medicinal value could underlay the breeding selection and relieve the pressure on wild resources. Methods Twenty-one Paris resources in the mountain area of Sichuan Basin were collected and selected according to their phenotype and rhizome features. Two resources GQ, MH with bigger rhizome and one resource (GK) with polygemmic feature were screened. After preliminary identification, based on Kimura-2-parameter model, molecular phylogenetic trees were constructed based on the different sequence of ITS and psbA-trnH between the screened resources and the homologous sequences from NCBI using the Maximum Like (ML) method. Main active saponin was determinated by HPLC method to predict its potential medicinal value. Results GQ, GK, and MH were special resources of P. polyphylla var. chinensis, P. polyphylla var. yunnanensis, and P. forrestii, respectively, in mountains around Sichuan Basin. The content and proportion of polyphyllin I, II, VI, VII in GQ, GK, MH were different. The total content was GQ > MH > CK > GK. The proportion of polyphyllin I in GQ and MH was 67.61% and 73.25% higher than CK, respectively. While the proportion of polyphyllin VII was most in GK (56.38%). Conclusion This study specified three Sichuan local Paris resources with excellent rhizome features. And they performed well after introduced to Chengdu Plain providing the material basis for the follow-up breeding study of Paris. Three resources have medicinal potential, especially the polyphyllin I and polyphyllin content in MH (P. forrestii) is higher, which can provide a new choice for screening substitution materials of P. polyphylla var. chinensis and P. polyphylla var. yunnanensis.
ABSTRACT
Objective: To prepare pH-dependent Paridis Rhizoma saponin (PRS) and Astragali Radix polysaccharide (ARP) colon targeting pellets for the treatment of colon cancer and finish its in vitro release performance evaluation. Methods: The colon targeted pellets were prepared with extrusion-spheronization and air-flow coating method and the the process parameters were optimized by orthogonal design. The coating fluid prescription was investigated by single factor test. In vitro release performance evaluation of the pellets was evaluated with polyphyllin I and II as the indexes. Results: The optimum technologic parameters of extrusion spheronization equipment were as follows: the rate of extrusion was 60 r/min, the rate of spheronization was 1 200 r/min, and the time of spheronization was 5 min. The optimum coating liquid formulation of pH-dependent colon targetting pellets was 15% weight gains of Eudrugit S100, 1.5% anti-plastering aid amount of Glycerin monostearate, and 5% plasticizer amount of TEC. In vitro release test showed that cumulative release rate of berberine hydrochloride was close to 0% in artificial gastric juice after 2 h and less than 10% in artificial intestinal fluid after 4 h, but the cumulative release rate in artificial colon juice after 2 h was more than 90%. Conclusion: The preparation method can be applied to the preparation of colon targeted pellets and the pellets can achieve the targeted release in the colon.