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Objective To study the chemical constituents of Hedyotis caudatifolia. Methods These compounds were isolated from H. caudatifolia and purified by repeated silica gel, polyamine, Sephadex LH-20 column chromatography and other methods. Their structures were identified by NMR, MS, and physico-chemical properties. Results Ten compounds were isolated and elucidated as hedycaffeoylglucoside A (1), β-sitosterol (2), 5α-stigmastane-3,6-dione (3), 7-hydroxy-6-methoxycoumarin (4), oleanolic acid (5), ursolic acid (6), pomolic acid (7), 3α,19α-dihydroxyurs-12-en-24,28-dioic acid (8), 2α,3β,24-trihydroxyolean-12-en-28-oic acid (9), and 2α,3α-dihydroxyolean-12-en-28-oic acid (10), respectively. Conclusion Compound 1 is a new caffeoyl glucoside and compounds 7-10 are isolated from this plant for the first time. Compound 1 exhibits cytotoxicity against four tumor cells.
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Objective To investigate the chemical constituents from the leaf of Camellia sinensis. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, ODS column chromatography, high pressure flash chromatography and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Nine compounds were separated from C. sinensis, among them, four compounds were identified as triterpenoids and named as 21β-hydroxyl pomolic acid (1), pomonic acid (2), pomolic acid (3), and ursolic acid (4); The other five compounds were idetified as triterpenoid saponins and named as 3-O-α-L-arabinopyranosyl pomolic acid (5), 3β-[(α-L-arabinopyranosyl)oxy]-urs-12,19 (20)-dien-28-oic acid (6), oleanolic acid-3-O-α-L-arabinopyranoside (7), 3β-[(α-L-arabinopyranosyl) oxy]-urs-12,18-dien-28-oic acid (8), and 20-epi-urs-12,18-dien-28-oic acid 3β-O-α-L-arabinopyranoside (9). Conclusion Compound 1 is a new compound, compounds 2, 3, 5-9 are obtained from this genus for the first time.
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Objective Seven triterpenes were isolated from the methanol extract of the roots of Rosa laevifgata. Methods The compounds were isolated and purified by a combination of various chromatographic approaches, including silica gel, Sephadex LH-20, semipreparative HPLC and so on. Their structures were identified on the basis of pfysicochemiscal and spedtroscopic analysis. The in vitro anti-inflammatory activity test was investjgated. Results On the basis of spectroscopic data analysis, their structures were identified as 18,19-seco-2α,3β,23α-trihydoxyl-19-oxo-urs-11,13(18)-dien-28-oic acid (1), (2R,19R)-methyl 2-acetyloxy-19-hydroxyl-3-oxo-urs-12-en-28- oic acid (2), pomonic acid (3), cecropiacic acid 3-methyl ester (4), 2-acetyl tormentic acid (5), pomolic acid (6), and 2α,3α-dihydroxyurs-12,18-dien-28-oic acid (7). Conclusion Among them, compound 1 named as rosasecotriterpene A is a new triterpene. The anti-inflammatory activities of compounds 1—7 are tested in vitro. The results show that all the isolated compounds have obvious inhibitory effects on the release of NO from RAW264.7 cells induced by LPS. The inhibitory effects of compounds 1 and 7 are more significant, showing good anti-inflammatory activity in vitro.
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Objective: To study the triterpenoids from the roots of Rosa laevigata. Methods: The silica gel column chromatography was used to extract and separate the chemical constituents from the roots of R. laevigata. HPLC was used to analyze its purity, chemical and spectroscopy methods were used to determine their structures. Results: Thirteen constituents were isolated and identified as niga-ichigosides F2 (1), rosamultin (2), arjunetin (3), kaji-ichigoside F1 (4), auscaphic acid (5), cecropiacic acid 3-methyl ester (6), 2-acetyl tormentic acid (7), pomolic acid (8), 2α,3α-dihydroxyurs-12,18-dien-28-oic acid (9), 3β-E-feruloyl corosolic acid (10), fupenzic acid (11), 2-O-acetyl euscaphic acid (12), and 12,13-dihydromicromeric acid (13). Conclusion: Compounds 3, 6, 7 and 9-13 are obtained from this plant for the first time. Compounds 10, 12 and 13 are obtained from the plants of Rosa L. for the first time.
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Objective: To study the triterpenoid components of Rosa cymosa. Methods: Compounds were isolated by repeated chromatography on silica gel column. The structures were elucidated by chemical and spectroscopic methods. Results: Thirteen triterpenoids were isolated and identified as 2-oxo-pomolic acid (1), 2α, 19α-dihydroxy-3-oxo-12-ursen-28-oic acid (2), 2-acetyl tormentic acid (3), pomolic acid (4), euscaphic acid (5), arjunic acid (6), myrianthic acid (7), arjunetin (8), rosamultin (9), kaji-ichigoside F1 (10), 2α, 3α, 19α, 23-tetrahydroxy-12-ursen-28-O-β-D-glucoside (11), fupenzic acid (12), and cecropiacic acid 3-methyl ester (13). Conclusion: Compounds 1-4, 7, and 11-13 are obtained from this plant for the first time.
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Objective: To study the chemical constituents from the cane of Pileostegia viburnoides. Methods: The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified on the basis of their physicochemical properties and spectroscopic data. Results: Thirteen compounds were isolated from the cane of P. viburnoides and their structures were identified as friedelin (1), 3-oxo-nofriedelin-28-al (2), stigmast-4-en-3-one (3), stigmasterol (4), (24R)-5A- stigmastane-3, 6-dione (5), tetracosyl amine (6), ursolic acid (7), pomolic acid (8), oleanolic acid (9), umbelliferone (10), 4-hydroxymellein (11), cleomiscosin A (12), and daucosterol (13), respectively. Conclusion: Compound 6 is obtained as a natural product for the first time; Compounds 2-5, 7-9, 11, and 12 are obtained from this genus for the first time and all the compounds are obtained from P. viburnoides for the first time.
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Objective: To study the chemical constituents in the effective fractions of charred Sanguisorbae Radix. Methods: The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results: Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17,19,21-trien (1), 3β-hydroxy-28-norurs-12,17-dien (2), 3β,19α-dihydroxyurs-13(18)-en-28-oic acid (3), 3β-[(α-L-arabin-opyranosyl) oxy]-28-norurs-12,17-dien (4), and pomolic acid (5). Conclusion: Compounds 1, 3, and 4 are novel compounds belong to triterpenoids and triterpenoid saponins, named as sanguisorbigenins Z, Y1, and Y2, respectively. © 2013 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
Objective: To study the chemical constituents from charred Sanguisorbae Radix. Methods: The compounds were isolated and purified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral analysis. Results: Five compounds were isolated and identified as 3β-hydroxy-28-norurs-17, 19, 21-trien (1), 3β-hydroxy-28- norurs-12, 17-dien (2), 3β-[(α-L-arabinofuranosyl) oxy]-28-norurs-12, 17-dien 3β, 19α-dihydroxyurs-13(18)-en-28-oic acid (3), 3β- [(α-L-arabinopyranosyl)oxy]-28-norurs-12, 17-dien (4), and pomolic acid (5). Conclusion: The compounds 1, 3, and 4 are novel compounds which belong to triterpenoids and triterpenoid saponins, named sanguisorbigenin Z, sanguisorbin Y1, and sanguisorbin Y2, respectively.