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Karanja Kshara is an alkali, extracted from water soluble ash of the plant – Karanja {Pongamia pinnata (L.)}. The Kshara preparation techniques differ according to various authors, with respect to the ratio of water added to ash, soaking time, folds of cloth and number of filtrations. In the current study, Karanja Kshara was prepared as per different classical methods and analysed for the differences. Three samples of Karanja Kshara were prepared, according to the textual references from Sushrutha Samhita (Sample A), Sharngadhara Samhita (Sample B) and Rasa Tarangini (Sample C). Analysis of the methods of preparation along with organoleptic and Physico-chemical tests were conducted. There was a 14% yield of Kshara in Sample A, 7.45% of yield in Sample B and 7.54% in Sample C. In physicochemical analysis, there was 6.8%, 6.1% and 5.4% of loss on drying in Samples A, B and C, respectively. Total ash value was 84.7%, 85.78% and 87.89% in Samples A, B and C respectively. Acid insoluble ash value was 11.34%, 11.04% and 9.15%. pH value was 10.36, 10.30 and 10.37. Traces of Sodium, Potassium, Magnesium, Calcium, Sulphur, oxides of silica, Carbonate & Bicarbonate were found in all the 3 samples. There are very few notable differences found in the results, however, they are quite similar. In terms of yield, economy and alkalinity, sample A (Sushrutha Samhita) can be considered as the better method while sample method C (Rasatarangini) is easier and faster.
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Pongamia pinnata (L.) Pierre is a medium sized glabrous, perennial tree which grows in the littoral regions of South Eastern Asia and Australia. In the Indian Ayurvedic medicine, different parts of the plant have been used for pain relief in various disorders. The present study investigated the potential of different leaf extracts of Pongamia pinnata as an analgesic agent in rodents and our aim was also to study in-vitro cytoprotective effects of the various extracts from leaves of the plant. Different leaf extracts of Pongamia pinnata i.e. aqueous, alcoholic, acetone and chloroform were investigated for analgesic activity at the dose rate of 50 mg/kg and 100 mg/kg in Wistar rats. For the assessment of analgesic activity, tail flick method was used. In-vitro cytoprotective activity of various leaf extracts (at concentrations of 5% and 10%) was evaluated in ATCC acquired MDBK cell lines and for this study, cytotoxicity was induced by thiomethoxam. It was observed that almost all the extracts demonstrated the dose dependent analgesic activity with maximum response in the aqueous extract group @ 100 mg/kg when compared to control. For cytoprotective study, oxidative stress parameters- catalase, LPO, SOD and GPx were determined. Study on analgesic activity revealed the presence of dose dependent effect in all extracts with highest effect in aqueous extract of Pongamia pinnata. We believe that triterpene alkaloids and steroidal principles present in the plant products might be responsible for the analgesic effect
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Pongamia pinnata L. (fabaceae) is a small evergreen tree reported in traditional literature as ‘Karanj’. Seeds of this plant have been used in the management of skin diseases, ulcer, piles, bronchitis; leprosy etc. Seeds are reported to possess phytoconstituents like pongapin, pongamol, pongaglabrone, kanjone, karanjin etc. The therapeutic activities of seeds are mainly attributed to its major furanoflavone karanjin. Thus, in the present research work the pharmacokinetics of karanjin was determined in Sprague dawley female rats using a validated RP-HPLC method from the ethanolic extract of P. pinnata (p.o.). The pharmacokinetic parameters were calculated using non compartmental model of analysis. Findings of the present work would be applicable for the future investigation on P. pinnata and karanjin for their different therapeutic uses and would be useful for justifying their dosage and route of administration from their allied formulations.
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Objective: To evaluate the mosquito larvicidal activity of Pongamia pinnata (P. pinnata) extracts against three mosquito vectors.Methods:The larval mortality was found in both methanol and hydroalcohol extracts of P. pinnata against fourth instar larvae of Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi. The mortality was observed 24 h and 48 h after treatment, data was subjected to probit analysis to determine lethal concentration (LC50 and LC90) to kill 50 and 90 percent of treated larvae of tested species.Results:The methanol and hydroalcohol extracts of bark part of P. pinnata L were tested against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi with LC50 values of 84.8, 118.2 and 151.7 ppm; 97.7, 128.3 and 513 ppm. The highest larval mortality was found in methanol extract of P. pinnata when comparable to the hydroalcohol extract.Conclusions:These results suggest that both methanol and hyrdoalcohol extracts have the potential to be used as an ideal ecofriendly approach for the control of disease vectors. This could lead to isolation of novel natural larvicidal compounds.
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Weaver ants, (Oecophylla smaragdina) have been found to predate on pentatomid bug (Cyclopelta siccifolia) infesting Pongamia pinnata tree in mid-western Ghat region of Shimoga district, Karnataka. The present study was undertaken to evaluate the potential of weaver ants, that are abundant on P. pinnata trees, to control the pentatomid bugs. Consistent and systematic field surveillance was carried out for a period of two months, March and July 2010. This showed that the number of pentatomid bugs colony found on branches of tree was significantly lower in tree with abundant weaver ants (11.21-16.84%) than in trees with fewer weaver ants (26.51-38.24%), or in trees without ants (61.42-75.47%) similarly, the branches damaged by pentatomid bug (9.42-11.43%) their in trees with abundant weaver ants which was significantly lower than in trees with fewer weaver ants (16.13-23.24%) and without ants (38.42-51.47%) in March and July. The pentatomid bug colony and their damage was significantly low on trees with abundant weaver ants than with fewer ants, or without the ants. Field observations regarding infestation of pentatomid bugs revealed that weaver ants are effective naturally occurring biological agents help in controlling damage caused by pentatomid bugs on Pongamia trees.
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The growth, yield, and carbon content of eight-month old seedlings of Pongamia pinnata were compared under water and urea supplementation. One set of plants were subjected to water stress condition (WS), whereas the other supplied with 2 g of urea (WS+U) under WS. Both the experimental set ups were exposed to varying treatment levels which include full irrigation (100%, control) followed by 75 % (T1), 50 % (T2), 25 % (T3) and 12.5 % (T4). The growth, leaf area and relative water content were maximum under WS when compared to WS+U (p<0.001). The maximum biomass was produced in the seedlings under WS in control (1.68 g) followed by T1 (1.38 g), T2 (1.53 g), T3 (0.93 g) and T4 (0.73 g). A significant (p<0.001) reduction in biomass production was observed in WS+U in control (1.28 g), T1 (0.66 g), T2 (1.13 g) and T3 (0.44 g). T4 of WS+U showed similar biomass (0.73 g) as that of T4 of WS. Under WS, the highest biomass allocation was recorded in shoots followed by leaves and roots. Similar trend was observed in WS+U. However, the percentage of allocation was more in the roots of WS+U (27.2 %) when compared to WS (22.24 %). The highest amount of carbon content was observed in control plants treated under WS (9.59 g) followed by control plants of WS+U (7.31 g) (p<0.001). The results of the preliminary study clearly indicated that P. pinnata seedlings were able to cope-up with water stress conditions without urea application and can perform well in 50 % water availability and is best suited for the plantation programs in the semi-arid ecosystems.
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This paper reports physicochemical studies in fungal infected galls of Pongamia pinnata. The parameters assayed were Total ash, acid insolubility and water solubility values and extractive values (Alcohol, water and petroleum ether). Fungal infected galls showed significantly higher value of ash, acid insolubility and low water solubility and high extractive values (petroleum ether and water) when compared to normal galls.
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Objective: To perform the pharmacognostic study of Pongamia pinnata (L.) Pierre (P. pinnata) stem bark. Method: The pharmacognostic studies were carried out in terms of organoleptic, macroscopic, microscopic, fluorescence analysis and physicochemical parameters. Results: The bark consisting of channelled, recurved, slightly quilled, usually 0.2-1 cm thick, lenticellate pieces with outer surface ash-grey to greyish-brown and internal surface yellowish-white to cream coloured having unpleasant odour and bitter taste. The main microscopic characterstics of the bark include phellem (5-20 or more layers of cork), phellogen (2-3 layered) followed by 10-15 layered phelloderm. Among other microscopic components were phloem parenchyma, phloem fibre and stone cells, traversed by wavy medullary rays. Further, physicochemical analysis of the bark power showed total ash, water soluble ash, acid insoluble ash and sulphated ash as 10.94, 1.96, 1.47 and 15.8 % w/w respectively. The alcohol and water soluble extractives values of the stem bark were 9.6 and 18.4 %w/w respectively. Conclusions: Various pharmacognostic characters observed in this study helps in botanical identification and standardization of P. pinnata L. in crude form.
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The production of a protease and a lipase from Bacillus pumilus SG2 on solid-state fermentation using Pongamia pinnata seed cake as substrate was studied. The seed cake was proved to be a promising substrate for the bacterial growth and the enzyme production. The initial pH, incubation time and moisture content were optimized to achieve maximal enzyme production. Maximum protease production was observed at 72 h and that of the lipase at 96 h of incubation. The production of protease (9840 U/g DM) and lipase (1974 U/g DM) were maximum at pH 7.0 and at 60% moisture content. Triton X-100 (1%) was proved to be an effective extractant for the enzymes and their optimal activity was observed at alkaline pH and at 60ºC. The molecular mass of the protease and lipase was 24 and 40 kDa, respectively. Both the enzymes were found to be stable detergent additives. The study demonstrated that inexpensive and easily available Pongamia seed cake could be used for production of industrially important enzymes, such as protease and lipase.
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Growing patterns of pediculocidal drug resistance towards head louse laid the foundation for research in exploring novel anti-lice agents from medicinal plants. In the present study, various extracts of Pongamia pinnata leaves were tested against the head louse Pediculus humanus capitis. A filter paper diffusion method was conducted for determining the potential pediculocidal and ovicidal activity of chloroform, petroleum ether, methanol, and water extracts of P. pinnata leaves. The findings revealed that petroleum ether extracts possess excellent anti-lice activity with values ranging between 50.3% and 100% where as chloroform and methanol extracts showed moderate pediculocidal effects. The chloroform and methanol extracts were also successful in inhibiting nymph emergence and the petroleum ether extract was the most effective with a complete inhibition of emergence. Water extract was devoid of both pediculocidal and ovicidal activities. All the results were well comparable with benzoyl benzoate (25% w/v). These results showed the prospect of using P. pinnata leave extracts against P. humanus capitis in difficult situations of emergence of resistance to synthetic anti-lice agents.
Subject(s)
Animals , Drug Evaluation, Preclinical , Insecticides/isolation & purification , Phthiraptera/drug effects , Millettia/chemistry , Plant Extracts/isolation & purification , Plant Leaves/chemistry , Survival AnalysisABSTRACT
AIM: To study the effect of extract from Pongamia pinnata roots on anti-inflammation and analgesia and acute toxicity. METHODS: The models of mice ear edema induced by xylene and Cotton pellet granuloma in rats to observe the anti-inflammation effect of PRE via oral administration. The effect of PRE on analgesia was tested by measuring the latent period licking hind foot with the hot plate method and counting body twisting induced by acetic acid in mice. The acute toxicity of PRE was measured by the method of Bliss. RESULTS: PRE could significantly inhibit the ear edema caused by xylene in mice, granuloma hyperplasia caused by cotton in rats. It could significantly prolong the pain threshold on hot-plate in mice, reduce the writhing times in mice. The LD50 of PRE was 6. 371 8 g/kg, its 95% confident limit was 5. 408 4-7. 723 2 g/kg. CONCLUSION: PRE has obvious effect on anti-inflammation and analgesia and the lower acute toxicity.
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AIM To investigate the therapeutic effect of ethyl acetate extract from Pongamia pinnata roots (PREA) on ethanol-induced gastric lesions. METHODS The experimental gastric mucosal injuries were prepared by ig ethanol to rats, and the protective effect of PREA was evaluated by calculating lesion index, observing pathological changes, and measuring the contents of nitric oxide (NO) and malondialdehyde (MDA), and the activity of superoxide dismutase (SOD) from gastric mucosal tissue. In addition, gastric secretary and gastric wall adherent mucus were studied with the pylorus-ligation rat model. RESULTSCompared with the model control group, PREA (50, 150 and 450 mg·kg-1, ig) dose-dependently prevented the gastric mucosal damages induced by ethanol, its inhibition rates were 28.7%, 57.7% and 78.7 %, respectively. The pathomorphology lesions of mucosal tissue were obviously ameliorated. PREA obviously antagonized the ethanol-induced elevation of MDA content, and reduction of NO level and SOD activity of gastric mucosa. PREA significantly reduced gastric juice volume, free acidity, total acidity and total acid output, but didn′t affect the pepsin activity. Moreover, PREA obviously increased adherent mucus quantity of stomach wall, as well as free mucus quantity dissolved in gastric juice of pylorus-ligation rat. CONCLUSIONPREA has protective effect on ethanol-induced gastric mucosal injuries, which suggests that PREA may be used for protection or treatment of human ethanolinduced gastric lesions.
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AIM: To study the effect of total flavonoids of Pongamia pinnata roots(PRF) on ulcerative colitis(UC) induced by dextran sulphate sodium(DSS) in mice and the possible mechanism. METHODS: Balb/c mice were fed with 4% DSS solution for 7 d to induce ulcerative colitis and treated with sulfasalazine(SASP) as positive control.The clinical symptom and the lesion of colonic mucosa were observed and the levels of T-AOC、MDA、NO and the activity of NOS in colonic tissue were tested. RESULTS: Compared with the model control,the clinical symptom and the lesion of colonic mucosa in treatment group were remarkably improved after administration of PRF in large or medium dose.In the two groups,the decrease in the levels of MDA,NO and the activity of NOS,and the increase in the levels of F-AOC could be found.(P0.05). CONCLUSION: The results indicate that significant effect of PRF on DSS-induced UC may attributae to reduction in free radical,depression of lipid peroxidation and enhancement of antioxidant capability.