Evidencia clínica e histológica de regeneración de tejido blando a partir de una matriz colágena porcina. Reporte de Caso

RESUMEN: La pérdida de un diente resulta en la pérdida de volumen de tejidos duros y blandos lo que dificulta lograr resultados estéticamente satisfactorios. Con el fin de disminuir la morbilidad que provoca un injerto autólogo en el sellado del alveolo se puede reemplazar por una matriz reabsorbible de colágeno. El presente reporte de caso evaluó clínica e histológicamente una matriz colágena de porcino, en la regeneración de tejido blando, durante la instalación de un implante inmediato a una extracción dentaria. A los 6 meses clínicamente se obtuvo un tejido con una apariencia estética final óptima e histológicamente se evidenció la formación de un tejido epitelial y conjuntivo compatible con la de una mucosa normal.

ABSTRACT: Tooth loss results in loss of hard and soft tissue volume, making it difficult to achieve aesthetically pleasing results. In order to decrease the morbidity caused by an autologous graft in the alveolus seal, it can be replaced by a resorbable matrix of collagen. The present case report evaluated clinically and histologically a porcine collagen matrix, in soft tissue regeneration, during the installation of an implant immediately after dental extraction. At 6 months, clinically, a tissue with an optimal final aesthetic appearance was obtained and histologically, the formation of an epithelial and connective tissue compatible with that of a normal mucosa was evidenced.

Collagen as a source of bioactive peptides: a bioinformatics approach

BACKGROUND: Collagen is the most abundant protein in animals and can be obtained from residues of the food industry. Its hydrolysate has many desirable properties that make it suitable as an additive in foods and cosmetics, or as a component of scaffold materials to be used in biomedicine. RESULTS: We report here the characterization of type I collagen from five different sources, namely bovine, porcine, chicken, trout and salmon, as well as their hydrolysates by means of bioinformatics tools. As expected, the results showed that bovine and porcine collagen, as well as trout and salmon collagen, can be used interchangeably due to their high identity. This result is consistent with the evolution of proteins with highly identical sequences between related species. Also, 156 sequences were found as potential bioactive peptides, 126 from propeptide region and 30 from the central domain, according to the comparison with reported active sequences. CONCLUSIONS: Collagen analysis from a bioinformatic approach allowed us to classify collagen from 5 different animal sources, to establish its interchangeability as potential additive in diverse fields and also to determine the content of bioactive peptides from its in silico hydrolysis.

O uso da matriz colágena porcina na reconstrução tecidual em Periodontia / The use of the porcin collagen matrix in the periodontal tissue reconstruction

A doença periodontal causa uma perda na estrutura de suporte dos elementos dentários. Como consequência, ocorre o aparecimento de sequelas provenientes dessa doença. Por conta disso foram desenvolvidos materiais com a fi nalidade de regeneração tecidual na estrutura de suporte, para que houvesse maior sobrevida desses elementos, aumento na quantidade de gengiva inserida e melhor otimização estética para os pacientes. Dentre os inúmeros materiais, temos a Matriz Colágena Porcina, que simplesmente é uma membrana obtida a partir de suínos, sendo que esta passa por uma cadeia de procedimentos, com a fi nalidade de minimizar e/ou eliminar qualquer tipo de interação alergênica no ser humano. A sua utilização é dada principalmente no aumento de tecidos moles ao redor de dentes afetados com a doença periodontal e em implantes osseointegrados, trazendo como principal vantagem a redução de morbidade do paciente, evitando, assim, a necessidade de um segundo sítio cirúrgico.

Periodontal disease results in the loss of structural support on dental elements. As such, sequels can be seen in the mouth. For this, materials were develop for tissue regeneration in order to increase the amount of attached gingiva and to optimize patient esthetics. The porcine collagen matrix is a product were the collagen undergoes a series of several treatments to minimize any type of allergic reaction to the human body. For example, it can be used for soft tissue augmentation around affected teeth and dental implants. Also, the porcine collagen matrix can reduce patient morbidity because it avoids autologous soft tissue harvesting.

Experimental study on long-term outcome of porcine collagen membrane xenotransplantation in vivo / 中国修复重建外科杂志

Methods: Twenty Sprague Dawley rats were randomly divided into 2 groups ( n=10). DermalGen were implanted subcutaneously into the dorsum of rats in experimental group, and the rats in control group were treated with sham-operation. At 3, 7, and 15 days and 1, 3, 6, and 12 months after operation, the samples of experimental group were harvested and gross observation, histological observation, CD31 immunohistochemical staining, and transmission electron microscope observation were taken to observe the inflammatory reaction, angiogenesis, and collagen arrangement. The skin tissues of the control group at 12 months were observed and compared.

Efeitos positivos preliminares de estratégias cirúrgicas e peri-implantares, combinadas para redução dos defeitos alveolares na zona estética provocados após trauma de avulsão, reimplantação dentária e reabsorção radicular / Positive preliminary effects of combined surgical and peri-implant strategies to reduce the alveolar defects at the esthetic zone after avulsion trauma, tooth replantation, and root resorption ­ a case report

Este relato de caso demonstra uma combinação de estratégias de RTG para alívio do defeito residual na zona estética. Um paciente do sexo masculino havia sofrido um acidente traumático no dente 11 durante a infância, com tratamento endodôntico e reimplantação imediata. Dez anos depois, o desnível entre as margens gengivais era de 10 mm, e uma reabsorção radicular estava presente. Para tentar diminuir a recessão de tecido mole e o contorno ósseo deficiente, uma matriz colágena suína (MucoGraft) foi montada no sentido vertical substituindo a parede vestibular, e o alvéolo de extração foi preenchido com osso bovino inorgânico (Bio-Oss). A coroa dentária natural foi usada como pôntico no aparelho ortodôntico de contenção. Três meses depois, e após um exame por tomografia computadorizada feixe cônico para determinação dos níveis ósseos resultantes, o conjunto implante dentário (3,4 mm x 10 mm)/pilar (platformswitching) e osso bovino foi colocado no alvéolo cicatrizado, e a coroa natural foi trocada por uma coroa provisória cimentada. Seis meses depois, um enxerto gengival livre foi aplicado. Após o condicionamento do tecido mole, o desnivelamento gengival entre as margens reduziu-se para 2 mm. A restauração definitiva recebeu infraestrutura em zircônia CAD/CAM recoberta por porcelana feldspática. Mesmo que a estratégia combinada tenha se mostrado útil no alívio da situação clínica, estudos longitudinais com amostras maiores são necessários para comprovar sua eficácia.

This case report demonstrates a combination of RTG strategies to alleviate a residual defect in the esthetic zone. A male patient had suffered a traumatic accident at the tooth 11 during childhood, with endodontic treatment and immediate replantation. Ten years later, the unbalance between the gingival margins was 10 mm, and a root resorption was detected. In an attempt to reduce the soft tissue recession and poor bone contour, a porcine collagen matrix (MucoGraft) was mounted vertically replacing the labial wall, and the alveolar extraction socket fi lled with xenogeneic bovine bone (Bio-Oss). The natural dental crown was used as the pontic in the orthodontic device. Three months later and after a cone beam computerized tomography to determine the resulting bone levels, the dental implant (3.4 mm x 10 mm)/abutment (platform-switching) assembly and bovine bone were placed in the healed socket, and the natural crown was replaced by a cemented temporary crown. Six months later, a free gingival graft was applied. After soft tissue conditioning, the unbalance between gingival margins between was reduced to 2 mm. The final restoration received a CAD/CAM zirconia infrastructure veneered with feldspathic porcelain. Even that the combined strategy has proven useful in this clinical situation. Longitudinal studies with larger samples are needed to confi rm its effectiveness.

Efficacy and Safety of Porcine Collagen Filler for Nasolabial Fold Correction in Asians: A Prospective Multicenter, 12 Months Follow-up Study

Recently, injectable dermal fillers have become important alternatives to surgical procedures for the correction of facial wrinkles. Bovine collagen is the first approved material for filler injection, and several studies have shown its efficacy. However, the risk of developing an allergic reaction and xenogenic transmission of bovine spongiform encephalopathy remain among its disadvantages. In this randomized, double-blinded, split-face study, we compared the efficacy and safety of a porcine collagen filler (TheraFill(R)) with that of a bovine collagen filler (KOKEN(R)) for nasolabial fold correction. A total of sixty one patients with mild to severe nasolabial fold were randomized to receive TheraFill(R) and KOKEN(R) on contralateral sides of the face. During the 12-month follow-up period, improvement in the Wrinkle-Severity Rating Scale score was slightly higher in TheraFill(R) group than KOKEN(R) group, although the difference was not statistically significant. No serious adverse reactions were observed and both materials were tolerable in most cases. In conclusion, the long-term effect of TheraFill(R) on nasolabial fold correction was comparable to that of KOKEN(R), and it may be a good alternative to bovine collagen filler.