Role of Fungal Rots in Post-harvest Storage Losses in Some Nigerian Varieties of Dioscorea Species.

Aims: To study storage rots in yam varieties cultivated in South East Nigeria and to determine under conditions of experimental storage, the influence of fungal rot on their post harvest storage losses. Place and Duration of Study: Laboratories, Department of Applied Microbiology & Brewing, Nnamdi Azikiwe University, Awka, Nigeria between January 2012 and July 2013. Methodology: Five yam varieties; Dioscorea dumentorum, two varieties each of D. alata and D. rotundata, obtained immediately after harvest were stored in an experimental barn (30ºC and 95% RH) and examined at intervals for storage rots. Fungal causative agents of rots were isolated and identified using the partial ITS rDNA sequencing analysis and a BLAST search using the GenBank sequence database. Post harvest storage losses in terms of weight loss and reduction of shelf life among the varieties were determined. Results: All varieties of yams studied suffered fungal rots, predominantly, dry rots during storage. Seven distinct fungal isolates, which caused these rots, were fully characterized. The species were Aspergillus tamari, Fusarium solani, Lasiodiplodia theobromae, Aspergillus niger, Mucor circinelloides, Aspergillus flavus and Aspergillus sp. In all the yams, storage rots reduced shelf life and aggravated weight loss. Post harvest storage losses varied among the different varieties of yams. Conclusion: The varieties of yams studied suffer rots from various fungi, which are similar to those reported in other parts of the world. Severity of post harvest losses resulting from fungal rots varies among different varieties of yams. This should be taken into consideration in the development of storage techniques.

Volatile organic compounds production during spoilage of African horned cucumber fruits.

Microorganisms associated with the production of volatile organic compound in spoilt African horned cucumber have been isolated by standard plate count (SPC) and identified. The mean aerobic colony count observed for bacteria ranged from 3.3 to 8.3 x 107 CFU/g while those of fungi ranged from 4.0 x 103 to 1.1 x 104 CFU/g. The organisms isolated and identified included three species of bacteria (Bacillus lentus, Bacillus firmus and Paenibacillus alveii) while three of fungi (Rhizopus stolonifer, Mucor circinelloides, and Monascus ruber). GC-MS analysis revealed the presence of 11 volatile organic compounds in the healthy ripe African horned cucumber and 21 volatile organic compounds in spoilt African horned cucumber. Six volatile organic compounds were common to spoilt and healthy African horned cucumber while 1,2,3, trimethyl benzene, Decane 1,4-Dimethyl-2-ethyl benzene, tetralin, 6- methyl tetralin, phenyl benzene (Lemonene), 2,3-dimethylnaphthale (Guajen), (1- Ethyl-2-methyl-1-propenyl) benzene, Diphenyl methane (Ditan), Hexadecane, Heptadecane, 6- octadecanoic acid (Z) and 1-Nondecanol were unique to spoilt African horned cucumber fruits. This study suggests that these unique volatile organic compounds could provide baseline knowledge for curbing postharvest losses and the volatile organic compound could form the basis for constructing a metabolomics database for Nigeria.

Tomato (Lycopersicon esculentum P. Miller) compression due to "K" wood box closing / Compressão de tomate (Lycopersicon esculentum P. Miller) em função do fechamento da caixa de madeira tipo "K"

Wood boxes kind "k" are the most used in table tomato (Lycopersicon esculentum P. Miller) commercialization in Brazil. Due to the fruit accommodation process that occurs during the transport from the production place to the commercialization point, producers fill tomato boxes a little bit over its physical capacity, and they close it with wood strips fixed with nails. In order to prevent strips from cracking, it is common to keep them inside water before using, to increase flexibility. When boxes are opened, many fruits are cracked or kneaded in a significant part of their total area, and normally they are thrown away. The purpose of this paper was to quantify the compression tomato fruits are exposed to in consequence of the "k" box fill / closing system. For this study a special instrumented box was constructed with a scales plate located at the bottom, with a hydraulic load cell instrumented in the box. This instrument was then used to study the compression of fruits as the box is filled and the compression increase caused by the addition of its wooden lid. The "k" box closing system caused an initial compression increase 3.5 times the compression of the open tomato box, and during time as the fruits are substantially deformed. This compression was reduced two about two times de initial reference value. The consequence of this observed fruit compression is compatible with the fruit deformations and even cracks observed in the market, which are known to be relevant post harvest losses causes in the Brazilian tomato handling industry.

Caixas de madeira do tipo "K" são as mais utilizadas na comercialização de tomate (Lycopersicon esculentum P. Miller) de mesa no Brasil. Em razão do processo de acomodação dos frutos que ocorre durante o transporte desde o local de produção até o local de comercialização, os produtores enchem a caixa de tomate um pouco acima de sua capacidade física e a fecham com ripas de madeira e pregos. Para evitar que as ripas rachem, é comum mantê-las imersas em água antes do fechamento, para que fiquem mais flexíveis. Quando as embalagens são abertas, muitos frutos estão rachados ou amassados em parte significativa de sua área e, normalmente, são descartados. Para este estudo, construiu-se uma embalagem cujo fundo é uma balança, com uma célula de carga hidráulica acomodada no fundo da caixa. Desse modo, a pressão que os frutos sofreram no interior da caixa com e sem o fechamento da tampa foi registrada ao longo do tempo, com auxílio de um tubo manométrico. O fechamento da caixa aumentou, inicialmente, em 3,5 vezes a compressão e depois, com a deformação plástica dos frutos, a compressão diminuiu para cerca de duas vezes em relação à compressão observada na caixa aberta. Portanto, as pressões observadas no fechamento da caixa comprimem os frutos de tomate, causando amassamento e até mesmo rachaduras. Estas injúrias têm sido observadas frequentemente na pós-colheita deste e outros produtos e são causas primárias de perdas consideráveis.