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Objective To investigate the correlation between prenatal infection and premature rupture of membranes (PROM).Methods Eighty-three cases of PROM in People's Hospital of Chengmai County from July 2014 to July 2015 were selected as observation group,and 80 cases of normal pregnant women were randomly selected as control group.Prenatal genital tract infection,neonatal morbidity,maternal serum C reactive protein(CRP) and white blood cell count level,maternal amniotic fluid cell factor level were compared between two groups.Results The total incidence rate in the control group was 23.75％,and the difference was statistically significant (P＜0.05).The incidence of respiratory distress syndrome,pneumonia and high serum bilirubin in the observation group was significantly higher than those in the control group (P＜0.05).The IL-6,IL-8 and TNF-o in the observation group were significantly higher than those in the control group (P＜0.05).Conclusion Prenatal reproductive tract infection and PROM are closely related.Infection is a factor causing PROM,which increases the rate of neonatal morbidity.Through prenatal test of blood CRP,amniotic fluid IL-6,IL-8,and detection of TNF-levels,the risk of PROM can be predicted in advance so that measures can be taken to avoid PROM and PROM-related maternal and fetal complications can be taken.
ABSTRACT
Objective To explore the changes of pubescent immune response in the schizophrenia offspring induced by poly(I:C) during pregnancy and the effects on white matter.Methods The obtained pregnant rats were randomly divided into model group(n=6) and control group (n=5), receiving either poly (I:C) at a dose of 10 mg/kg diluted in 0.9％ NaC1 solution or vehicle solution alone (sterile pyrogen-free 0.9％ NaC1) on gestation day 9 (GD9).Immunohistochemical technique(IHC) was applied to detect the changes of microglias and astrocytes in the prefrontal cortex(PFC) and hippocampus(HC) of partly offsprings in the two groups at the sixth week,as well as Luxol fast blue(LFB) for the changes of white matter.The other offsprings of each group were selected for behavioral assessment at the eighth week.Results The results of prepulse inhibition test showed that PP2, PP4 and PP8 of model groups were significantly lower than that of the control group at young adult(P＜0.01).In passive avoidance test, and the T1 results of model group were significantly higher than those of the control group, the T results of model group were lower than those of control group (P＜ 0.01).Immunohistochemical results indicated that the number of microglias in the model group((264±33)/mm2, (271 ±38)/mm2) was significantly increased in PFC and HC than that in the control group((140±29)/mm2, (169±37)/mm2, P＜0.05) ,which was accompanied with significant morphological changes, while the OD value of astrocyte protein expression in the frontal lobe and hippocampus had no obvious difference between the model group and control group(P＞0.05).The OD value of LFB staining for myelin in the model group(0.29±0.02) was significantly decreased compared with that in the control group(0.33±0.03)(P＜0.01).Conclusion The young adult offsprings with prenatal infection present obvious schizophrenia-like behavior, meanwhile, the microglias activation and demyelination changes in white matter are observed,which provides more evidence for the relationship between immune response and white matter in the pathogenesis of schizophrenia.