ABSTRACT
Objective To explore the change of conventional biochemical test results of serum specimens saved-20℃ and blending of its effects.Methods The fresh serum of 24 patients were conducted routine biochemical tests,respectively in four groups of numbers in the same capacity of 1 ml of the sample tube,a set of 25℃ kept 24 hours a day,a group of 4 ℃ refrigerated for 24 hours,24 hours at-20℃ save the two groups (a group was not incorporated,a set of blended),and the four groups of serum samples in biochemical results comparing with the initial results.Results 25℃ at room temperature preservation after 24 hours,ALT,AST,TBIL and DBIL biochemical indicators of four average were below the initial results (t=2.130,2.308,2.130 and 2.308,P=0.042,0.029,0.042 and 0.003),there were no statistically significant difference between the rest of the biochemical index results comparison (P＞ 0.05).4℃ refrigerator after 24 hours,TBIL,DBIL levels below the initial level (t=2.103 and 2.089,P=0.045 and 0.046),and the rest of the biochemical indicators to compare differences had no statistical significance (P＞0.05).-20℃ refrigerated in the two groups of samples,not each sample of serum biochemical results compared with the initial results,were kept statistically significant difference (t=-4.218～11.710,all P＜0.05),while serum samples of 15 items and biochemical indexes of blending the results compared with the initial results,the average level of had no statistical significance (P＞0.05).Conclusion Serum samples of the chemical composition of the homogeneity in the 4 ℃ refrigerator will not change,but will not be able to timely detection of serum samples in the preservation,and minus-20 ℃ refrigerator on the serum samples of a significant difference was found in the lower concentration of chemical components,compete on low,and tests conducted before fully blending can ensure the precision of its biochemical test results.
ABSTRACT
Objective Beagle dogs are commonly used animal for drug safety evaluation .As the necessary parameters , blood biochemical indicators are detected in acute or chronic toxicity tests .This study aims at assessing the influence of different preservation conditions and different preservation time on blood biochemical indicators to ensure the reliability of test results of long-term toxicity assessment .Methods Six Beagle dogs (3 males and 3 females) were used in this study .After collection and preparation of serum samples , biochemical indicators were detected after preservation in refrigerator at 2-8℃for 1, 2, 5, 8, and 12 hours;after preservation in ice transportation boxes at 2-10℃for 2, 5, and 8 hours;and after preservation in refrigerator at -20℃ for 1, 3, and 5 days.The biochemical indicators included alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) and alkaline phosphatase ( ALP ) , total protein ( TP ) , albumin (propagated), urea, creatinine (CREA), glucose (GLU), total cholesterol (TCHO), total bilirubin (TBIL), creatine kinase ( CK ) , gamma pancreatic acyl transferase ( GGT ) , calcium ( CA ) , lactate dehydrogenase ( LDH ) , phosphorus ( P) , high-density lipoprotein cholesterol ( HDL-C) , low density lipoprotein cholesterol ( LDL-C) , triglyceride ( TG) , sodium ( Na+) , potassium ( K+) and chloride ( Cl -) .Results Compared with the results of samples preserved for 1 hour, the LDL-C result of that preserved in refrigerator at -20℃for 5 days was significantly increased (P0.05 ) , and the coefficient of variation of LDH was 41%.Conclusions According to the test results of blood biochemical indicators in the Beagle dogs detected after different preservation conditions and different preservation time in this study , detection test should be done within 1 hour, if not, detection should be done within 12 hours for the samples preserved at 2~8℃, or within 3 days for the sample preserved at -20℃.For transportation of serum samples , the serum samples should be placed in the ice box at 2~10℃, and detection test should be done within 8 hours .