ABSTRACT
OBJECTIVE:To optimize the water-extraction technology of Compound kuhuang formulation. METHODS:The wa-ter-extraction technology of Compound kuhuang formulation was optimized by orthogonal design,with the total contents of matrine and oxymatrine,the total content of prim-O-glucosylcimifugin and 5-O-methylvisamminol,the content of berberine hydrochloride and extract yield,and with the extraction time,amount of water(3 reflux extractions)and soaking time as the factors;and verifi-cation tests were conducted. RESULTS:The extraction time had significant effect on comprehensive evaluation value (P<0.05). The optimal water-extraction technology was as follows as extraction time of 120 min,water amount of 8,6,and 6 times as the amount of herbs,and soaking time of 20 min. In verification tests,the average total content of matrine and oxymatrine was 3.152 6 mg/g(RSD=1.03%,n=3),that of prim-O-glucosylcimifugin and 5-O-methylvisamminol was 4.977 2 mg/g(RSD=2.27%,n=3),the average content of berberine hydrochloride was 3.345 0 mg/g (RSD=1.19%,n=3) and the average extract yield was 49.23%(RSD=2.43%,n=3). CONCLUSIONS:The optimal water-extraction technology of Compound kuhuang formulation is stable and feasible.
ABSTRACT
High-speed counter-current chromatographic ( HSCCC ) method was successfully used to separate and purify prim-O-glucosylcimifugin and 5-0-methylvisammioside from Saposhnikovia divaricata(Thicz. ) Schis-chk. ,a traditional Chinese herb,with a solvent system composed of ethyl acetate-n-butanol-water(2:7:9,V/V). The lower phase of the system was used as the mobile phase at the flow rate of 2.0 mL/min,and the upper phase was used as the stationary phase. The separation produced a total 13.9 mg prim-O-glucosylcimifugin and 25.0 mg 5-0-methylvisammioside with the purity of 98. 1% and 99.2% determined by high performance liquid chromatography (HPLC) from 316 mg of crude sample from S. divaricata. The structures of isolated compounds were identified by ESI-MS,~1H NMR and ~(13)C NMR.
ABSTRACT
OBJECTIVE: To establish a method for the content determination of calycosin-7-O-?-D-glucoside,prim-o-glucosyl cimifugin and 4′ -O-?-D-glucosyl-5-O-methylvisammin in Yupingfeng decoction and to study the influence of different combination on the content of three compositions.METHODS:HPLC was applied and the determination was performed on Hypersil ODS(250 mm?4.0 mm,5 ?m)column.The mobile phase consisted of acetonitrile-water(gradient elution)with detection wavelength of 254 nm.RESULTS: The content of the three compositions represented little change or different deceasing tendency in the decoctions of various compatibilities.The biggest deceasing tendency of content was found in the decoctions containing Astragalus membranaceus,Saposhnikovia divaricata,and Atractylodes macrocephala.CONCLUSIONS: The content of the three compositions of different combination shows a dynamic course,which provides a reference for the rules of the compatibility and material basis of Yupingfeng decoction.