Modulatory effect of progesterone on peripheral pain / 第二军医大学学报

Objective: To investigate the modulatory effect oí progesterone on peripheral pain. Methods: Female SD rats were divided into four groups, namely, a vehicle group, a progesterone (500 μg) group, an RU38486 (20 μg) group and a progesterone (500 μg) plus RU38486 (20 μg) group. Von-Frey hair test was adopted to determine the peripheral mechanical pain thresholds at the following 3 ime points: preinjection, 30 min and 60 min after subcutaneous injection at the neck. Local effect of progesteronewas also tested in SD rats by Von-Frey hair test 15 min after plantar injection of progesterone (25 μg) and normal saline (control). Results: The peripheral mechanical pain threshold was significantly increased 30 min after subcutaneous injection of progesterone at the neck compared with the vehicle group, but the threshold was not significantly different 60 min after injection. RU38486 injection at the neck significantly decreased the pain threshold of plantar at 30 min and 60 min after injection, and the peripheral mechanical pain threshold was not significantly altered at 30 min and 60 min after coinjection of progesterone and RU38486. RU38486 could block the pain inhibiting effect of progesterone. The peripheral mechanical pain threshold was also increased 15 min after plantar injection of progesterone compared with the control group. Conclusion: Our findings suggest that progesterone can inhibit the peripheral mechanical pain, which might be mediated by progesterone receptor.

Clinical Significance on the Expression of Estrogen and Progeste rone Receptors in Endometrial Carcinoma,Atypical Hyperplasia and Normal Endome trium / 医学研究杂志

The purpose of this article is to d iscuss the meaning and the method of testing the ER/PR.We use the anti-ER、 ant i-PR monoclonal antibody SP method to detect the ER/PR in 26 cases of the endome trial cancinoma endometrium、23 cases of the atypical hyperplasia and 22 cases o f the normal endometrium respectively.The result shows that the SP method to t est the ER/PR has different result in carcinoma、atypical hyperplasia and normal endometrium.The expression of ER/PR in carcinoma is distinctly lower than th at in the atypical hyperplasia and normal endometrium.There is no significant d ifferent between the atypical hyperplasia and normal endometrium of the expressi on of ER/PR.However,there are significant different in the ER.PR expressions b e tween normal endometrium and endometrial careinoma,and between atypical hyperpla sia and endometrial careinoma,reflecfing the special biological action of the tu mor.SP method to test the expression of ER/PR has some special meaning to estim ate the prognosis and supervises the treatment of the carcinoma of the uterine.

The Difference of Cathepsin D Expression between Invasive Ductal Carcinoma and Ductal Carcinoma In Situ of the Breast

BACKGROUND: It is known that cathepsin D expression in host stromal cells is associated with a more aggressive tumor behavior in breast cancers. METHODS: Cathepsin D expression was examined in 222 cases of invasive ductal carcinoma (CA) and 25 cases of ductal carcinoma in situ (DCIS) by the immunohistochemical staining. Cathepsin D expression was evaluated according to the expression site, either in the tumor cells (CD-T) or in the stromal cells (CD-S), and graded according to the immunopositivity. The differences of CD-T and CD-S in each case were evaluated according to the pathologic parameters and estrogen receptor (ER)/progesterone receptor (PR) status. RESULTS: The rate of CD-S was significantly higher in the CA than in the DCIS (p<0.0001). In the CA, the rate of CD-S was higher than that of CD-T, while in the DCIS, the rate of CD-T was higher than that of CD-S. In the CA, the rate of CD-S and the tumor grade showed a positive relationship (p=0.0281). There were positive correlations between the ER positivity and CD-S (p=0.0236), and between the PR positivity and CD-T (p=0.0246). For the DCIS, no significant relationships were noted between the pathologic parameters including ER/PR status and CD-T/CD-S. CONCLUSION: Cathepsin D expression in the stromal cells seems to be related to the invasiveness and aggressive biological behavior in breast cancers. In addition, there might be some relationship betweeen the ER positivity and CD-S, and between the PR positivity and CD-T.

Correlation between Expression of Insulin-like Growth Factor-I Receptor and Clinicopathologic Prognostic Factors in Invasive Ductal Carcinoma of the Breast

PURPOSE: The insulin-like growth factor-I receptor (IGF-IR) is a member of the transmembrane tyrosine kinase family that regulates various biological processes. These processes include maintaining optimal cell growth, establishing and maintaining the transformed phenotype, tumorigenesis for several types of cells, and antiapoptosis. This study investigated the immunohistochemical expression of IGF-IR in relation with the expression of the estrogen receptor (ER), the progesteron receptor (PR), proliferative activity (Ki-67) as well as with the other conventional clinicopathlogical parameters in breast cancer. METHODS: This study was performed on paraffin sections from 64 invasive ductal breast carcinoma specimens by immunohistochemistry using rabbit polyclonal antibodies to the IGF-I receptor. RESULTS: IGF-IR expression was detected in 56 (87.5%) cases. The immunohistochemical stains for the IGF-IR were scored according to a semi quantitative scoring system. IGF-IR staining was positively correlated with the ER status, but not significantly with the PR, lymph node status, tumor size, histological grade, and proliferative activity. The Ki-67 labeling index showed a significant correlation with the tumor grade and ER status. CONCLUSION: The IGF-IR may play a role in estrogen-mediated growth and the pathogenesis of breast cancer.

Correlation between Expression of Insulin-like Growth Factor-I Receptor and Clinicopathologic Prognostic Factors in Invasive Ductal Carcinoma of the Breast / 한국유방암학회지

PURPOSE: The insulin-like growth factor-I receptor (IGF-IR) is a member of the transmembrane tyrosine kinase family that regulates various biological processes. These processes include maintaining optimal cell growth, establishing and maintaining the transformed phenotype, tumorigenesis for several types of cells, and antiapoptosis. This study investigated the immunohistochemical expression of IGF-IR in relation with the expression of the estrogen receptor (ER), the progesteron receptor (PR), proliferative activity (Ki-67) as well as with the other conventional clinicopathlogical parameters in breast cancer. METHODS: This study was performed on paraffin sections from 64 invasive ductal breast carcinoma specimens by immunohistochemistry using rabbit polyclonal antibodies to the IGF-I receptor. RESULTS: IGF-IR expression was detected in 56 (87.5%) cases. The immunohistochemical stains for the IGF-IR were scored according to a semi quantitative scoring system. IGF-IR staining was positively correlated with the ER status, but not significantly with the PR, lymph node status, tumor size, histological grade, and proliferative activity. The Ki-67 labeling index showed a significant correlation with the tumor grade and ER status. CONCLUSION: The IGF-IR may play a role in estrogen-mediated growth and the pathogenesis of breast cancer.

Estrogen Receptor alpha, beta and Progesteron Receptor Expression in Gynecomastia Using Immunohistochemical Staining

PURPOSE: Gynecomastia is a common male breast abnormality and primarily occurs in puberty and senescence. The obvious etiological role of hormonal changes in gynecomastia, plus the discovery of estrogen receptor in normal and neoplastic breast, has spurred several investigations of ER content in male gynecomastic tissues. The results have been inconsistent and the fraction of ER-positive specimens has varied from 0~90%. METHODS: Immunohistochemical hormonal receptor analysis using monoclonal estrogen receptor (ER) alpha, beta and progesteron receptor (PR) was performed on the breast tissues of 58 patients with gynecomastia between January 1995 and January 2000 in the Department of Surgery, Uijongbu St. Mary's Hospital. These results were statistically compared with clinical data. RESULTS: 48 cases (82.8%) were ERalpha positive and 55 cases (94.8%) were ERbeta positive and PR positivity was noted in 55 cases (94.8%). There was negative relationship between ERalpha and age, PR and location. CONCLUSION: This study demonstrates that intracellular steroid receptors are present in most gynecomastic tissues. Additionally, it supports the general assumption that estrogen and progesteron may be two of the hormones responsible for the development of gynecomastia.

An Immunohistochemical Studcy of Estrogen-receptors and Progesterone-receptors Expression in Pyogenic Granuloma / 대한피부과학회지

BACKGROUND: Pyogenic granuloma(PG) is a common lesion of the skin and mucous membranes. The gingival lesion developed during pregnancy termed epulis gravidarum is identical to PG. Many articles have appeared in the literature pertaining to this lesion and its putative relationship to the hormonal changes of pregnancy. Several clinical features such as association with oral contraceptive use and regression after delivery, suggest that PG may be a hormone-sensitive lesion. OBJECTIVE: Our aim was to determine whether estrogen or progesterone might affect the development of PG. METHODS: We performed immunohistochemical staining by using a monoclonal antibodies to estrogen receptor(ER) and progesterone receptor(PR) in 15 PG(pregnant women; 4 cases, non-pregnant women; 5 cases, and men; 6 cases). RESULTS: All 15 PGs were negative for ER. However, for PR, the degree of staining was different according to the patient group; pregnant women(3 cases[75%]: weak positive, 1 case[25%]: strong positive), non-pregnant women(3 cases[60%]: weak positive), and men(6 cases[100%]: negative). CONCLUSION: Our results suggest that estrogen or progesterone may not directly involve in the formation of these lesions. Further studies are needed to determine whether the other factors are related to the pathogenesis of PG.