Expression of p63,bcl-2,bcl-6 and p16 in Basal Cell Carcinoma and Squamous Cell Carcinoma of the Skin

BACKGROUND: Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) are the common malignant neoplasms of the skin. The p63 is a p53 homologue which is considered to be a reliable keratinocyte stem cell marker. Bcl-2 plays a key role in cell longevity by preventing apoptosis, whereas the bcl-6 gene functions as a transcriptional repressor. The p16-CDK4/6 complex arrests the cell cycle at G0 /G1 phase. In the present study, the expression of p63, bcl-2, bcl-6, and p16 in BCC and SCC was evaluated. METHODS: Forty-seven BCCs and 43 SCCs were selected and microarrayed in paraffin blocks. Immunohistochemical analysis was performed with specific antibodies for bcl-2, bcl-6, p16 and p63. RESULTS: p63 was found to be expressed in all BCCs and SCCs. Bcl-2 was exclusively expressed in BCCs (100%), but there was negative expression in SCCs, whereas bcl-6 was positively expressed in 18.2% of SCCs, and was negative in BCCs. In SCCs, p16 was expressed at high frequency (47.7%) than in BCCs (14.9%). The expression of p16 was correlated with the histologic grades of SCCs. CONCLUSION: The different patterns of bcl-2, bcl-6, p63 and p16 protein expression between BCCs and SCCs may represent the different histogenesis and morphologic features of two lesions.

Immunohistochemical Study on bcl-2, c-myc, EGFR as Predictable Factor for Radiosensitivity in Early Glottic Cancer / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: The radiotherapy or surgery is the main treatment modality for early glottic cancer. The advantage of radiotherapy is the preservation of good voice quality after treatment but the main problem is increased complications in the salvage surgery when local control fails. So, it is important to predict the success of radiotherapy. The purpose of this study is to find predictable factors of the radiosensitivity in the early glottic cancer. MATERIALS AND METHOD: Immunohistochemical staining was performed on the paraffin sections of the biopsy specimens of 57 patients with early glottic cancer who had undertaken radiotherapy treatments in Chonnam National University Hospital from January 1988 to October 1998. Primary antibodies were the anti-bcl-2 monoclonal antibody, the anti-c-myc monoclonal antibody, and the anti-EGFR monoclonal antibodies. The relation between the local control outcome after radiotherapy and the result of immunostaining was analyzed by the chi-square and the Fisher's exact test. RESULTS: Positive expression of bcl-2 was 21.1% in the local controlled group and 12.3% in the uncontrolled group. There was no statistical significance between two groups. The expression rate of c-myc was statistically higher in the controlled group (36.8%) than in the uncontrolled group (7.0%) (p=0.025). Expression of EGFR was 57.9% in the controlled group and 22.5% in the uncontolled group. However, there was no statistical significance between two groups. CONCLUSION: The relation between the positive c-myc expression and the radiosensitivity suggests that c-myc might be a predictable factor of the radiosensitivity in early glottic cancer.

Immunohistochemical Study on Bcl-2 and PCNA in Mixed Tumor of Skin / 中华皮肤科杂志

Objective To investigate the expression of bcl-2 and PCNA in mixed tumor of skin. Methods Bcl-2 and PCNA expression in 12 cases of mixed tumor of skin were studied using immunohistochemical S-P method and double-staining. Results Positive reactions of bcl-2 and PCNA were observed in all cases. PCNA staining was positive in tumor cells lining cystic and duct-like structures in tumor nests, but bcl-2 staining was positive in epitheloid tumor cells. Most tumor cells with bcl-2 were PCNA negative and vice versa. Conclusion Aberrant expression of bcl-2 and PCNA in mixed tumor of skin may play a role in tumorigenesis.