ABSTRACT
Background: Drug induced nephrotoxicity, one of the most common renal problem, is a challenge to deal with especially in patients with renal dysfunction. It is responsible for 20% cases of acute renal failure in the community. Modern medicines are costly and have minimal nephroprotection. Solanum nigrum fruit extract, a cheaper drug, have antioxidant property and may help in nephroprotection.Methods: Total 54 rats were randomised in 3 groups named G10, G20 and G30 according to 10, 20 and 30 days of treatment. In each groups, rats were randomly assigned to any of the three subgroups i.e., control C group [received normal saline (2 ml/100 gm/day) orally consecutively for test duration], gentamicin treated (GT) group [received normal saline (2 ml/100 gm/day) orally consecutively for test duration and intraperitoneal gentamicin (40 mg/kg) once daily for last five days] and S. nigrum treated (SNT) group [received S. nigrum orally (200 mg/kg/day) for the test duration and intraperitoneal gentamicin (40 mg/kg) once daily for last five days]. Rats were sacrificed 24 hours after the last dose of gentamicin injection (on 11th, 21st and 31st day). Excised kidneys were weighted and prepared for histological examination.Results: The mean weight of kidneys in GT group was significantly higher than the SNT group in all test durations suggestive of decrease in inflammation in SNT group. This was also reflected histologically as SNT group kidney showed less amount of tubular destruction as compared to GT group.Conclusions: S. nigrum extract provide nephroprotection against gentamicin induced nephrotoxicity.
ABSTRACT
Introduction: The description of development of human kidney given in various textbooks doesn’t include detail microscopic appearance of kidney at various fetal ages. So an attempt was made in this study to gather information on this topic. Material and methods: The present study was carried out on 15 human fetuses of known gestational age (GA). The sections of kidney were processed and were stained using Hematoxylin and Eosin. Results: At 12th week of GA various stages of developing glomeruli were observed in the substance of the kidneys. In the cortex, various cut sections of the tubules were observed without any differentiation as proximal (PCT) and distal tubules (DCT). The second trimester section showed well differentiated the PCT and DCT by 16th week. Distinct brush border was observed in PCT by the 16th week. Immature duct system was observed in the medulla. The nephrogenic zone was appreciated till 36 weeks. By 28 th week the sections of DCT were observed adjacent to the renal corpuscles indicating the developing juxtaglomerular apparatus. Conclusion: As it is essential to know the developmental morphology of kidney, the present study explains every component of it in detail.
ABSTRACT
Experimental studies suggest that captopril plays an important preventive role in radiation induced renal injury (RRI). To elucidate the pathogenesis of RRI and effect of captopril, one subgroup was irradiated with a single dose of 9 gray (Gy) total body irradiation and another subgroup with 17 Gy local irradiation in the right kidney. Twenty-four healthy looking Sprague-Dawley rats, weighing 200~250 g, were divided into one control and three experimental group (EG)s for this study. The control group, composed of 2 rats, was maintained on stock diet and drinking tap water. EG was divided into three. EG 1 composed of two subgroups, the first subgroup, 3 rats each, was sacrificed within 12 hours after 9 Gy and 17 Gy single dose irradiation only and the second subgroup, 2 and 1 rats each, was sacrificed 8 weeks after the same doses irradiation. EG 2 composed of subgroups of 2 and 3 rats was given 500 mg/L of captopril in the drinking water after irradiating them with 9 Gy and 17 Gy and sacrificed in the 8th week. EG 3 was subdivided into four subgroups by captopril doses given, 62.5 mg/L, 125 mg/L and 250 mg/L and sacrificed 20 weeks after 9 Gy and 17 Gy irradiation. On light microscopy proximal convoluted tubules showed cytoplasmic vacuolization and focal necrosis in the subcapsular region in EG 1 sacrificed within 12 hours after 9 Gy and 17 Gy irradiation only (sham) and very mild fibrosis in juxtamedullary regions in rats sacrificed 8 weeks after irradiation. In EG 3 these changes were severely increased with additional increased fibrosis in the juxtamedullary region in the group given captopril 62.5 mg/L. On transmission electron microscopy, there were various degenerative changes of organelle. Among the captopril administered EG 2 and EG 3, rats given a high dosage revealed milder degree of damage compared to that of rats given a low dosage, and thickening of basement membrane was remarkable in rats given a low dosage. There was a reduction in tubular damage related to the captopril dosage. According to the above findings, administration of a high dose of captopril might preserve the ultrastructure in RRI and the possible mechanism of captopril was discussed.