Multiple Mild Stimulations Reduce Membrane Distribution of CX3CR1 Promoted by Annexin a1 in Microglia to Attenuate Excessive Dendritic Spine Pruning and Cognitive Deficits Caused by a Transient Ischemic Attack in Mice / 神经科学通报·英文版

A transient ischemic attack (TIA) can cause reversible and delayed impairment of cognition, but the specific mechanisms are still unclear. Annexin a1 (ANXA1) is a phospholipid-binding protein. Here, we confirmed that cognition and hippocampal synapses were impaired in TIA-treated mice, and this could be rescued by multiple mild stimulations (MMS). TIA promoted the interaction of ANXA1 and CX3CR1, increased the membrane distribution of CX3CR1 in microglia, and thus enhanced the CX3CR1 and CX3CL1 interaction. These phenomena induced by TIA could be reversed by MMS. Meanwhile, the CX3CR1 membrane distribution and CX3CR1-CX3CL1 interaction were upregulated in primary cultured microglia overexpressing ANXA1, and the spine density was significantly reduced in co-cultured microglia overexpressing ANXA1 and neurons. Moreover, ANXA1 overexpression in microglia abolished the protection of MMS after TIA. Collectively, our study provides a potential strategy for treating the delayed synaptic injury caused by TIA.

The roles of GluN3-containing N-methyl-D-aspartate receptor in central nerve system / 浙江大学学报·医学版

The N-methyl-D-aspartate receptor (NMDAR) in central nerve system is mostly composed of GluN1 and GluN2 subunits. The classical NMDAR has been intensively studied. However, GluN3‑containing NMDAR is much less expressed and have atypical channel properties. Recently, accumulating evidences have revealed two types of GluN3‑containing NMDAR: glutamate-gated GluN1/GluN2/GluN3 NMDAR and glycine-gated GluN1/GluN3 NMDAR. The former may play important roles in regulating synapse maturation and pruning non-used synapses, and its elevated expression at the adult stage may alter synaptic reorganization in some neuropsychiatric disorders. The latter is expressed in the medial habenula and involves in control of aversion. This article reviews the recent progresses on the expression, functional properties of GluN3‑containing atypical NMDARs and the physiological and pathological relevance.

Synaptic pruning mediated by glia in Alzheimer's disease / 药学学报

Alzheimer's disease (AD) is a neurodegenerative disease characterized by memory loss and cognitive impairment. To date, however, no disease-modifying strategies to prevent or cure AD exist. Synapses are involved in the connection of neurons and present as the key component for the memory and other neural activities. Synapse loss is a critical hallmark of AD pathology. In brain, glia cells, including microglia and astrocytes, are a group of highly specific cell types other than neurons. Microglia and astrocytes play a key role in maintaining the healthy neural circuit and regulating synaptic plasticity. Under development and physiological conditions, glial cells contribute to construct and maintain mature central neural networks via synaptic pruning. However, during AD pathogenesis, glial cells engulf synapses excessively, which leads to synapse loss, neuronal dysfunction, and cognitive impairment. Here, we review recent advances in our understanding of the underlying mechanisms for glia-mediated synaptic pruning in AD, and provide a novel strategy for the development of AD drugs.

Ketamine exhibit antidepressant effect through regulating sleep-wake rhythm and synaptic homeosta-sis / 中华行为医学与脑科学杂志

A single infusion of ketamine has sustained antidepressant effects and significantly de-creases the risk of suicide,and the effects can last up for 7-10 days,but the underlying mechanism is un-clear. The mechanism was reviewed underlying the antidepressant effects of ketamine,and found that ket-amine may exert its antidepressant effect by regulating sleep/wake cycle,synaptic pruning,molecular path-ways,and neural circuits for treatment-refractory depression. Further studies are needed to investigate the ge-netic,molecular mechanisms underlying the sustained antidepressant effect of ketamine,and the associated imaging findings through in vivo imaging of animals and imaging genetics techniques,explore the optimal time for administration of ketamine,and then provide accurate scientific basis for enhancing its anti-depressant effect.

Methods for assessing resources of forestry residues / 生物工程学报

In this study, models for assessing resources of forestry residues were improved based on analysis of previous studies and current forest production in China. And novelties are highlighted: this study covered the models for the calculation of 10 secondary forestry residues from woody nursery, forest woody pruning, wood logging, firewood, wood bucking, wood handling, waste wood, banana and pineapple plants, bamboo processing and waste bamboo; the residues produced from fast-growing forest for logging and processing of imported wood were taken into consideration to calculate woody residue potential; the calculation of pruning residue included woody fruit trees, and herbaceous fruit plants from the management of orchard and other economic forest; the consistency of parameter terminology and definition which referred from their literature resources have been ensured; more studies focused on the coefficient values, industrial standard, forestry residue amount and spatial distribution are recommended for future researches.

Microglia and synaptic pruning in developmental brain / 中国病理生理杂志

Microglia, the main innate immune cells in the central nervous system, takes part in lots of physiological and pathological processes in the brain.It not only maintains brain homeostasis but also participates in the process of brain injury and repair under pathological conditions.In developmental brain, microglial synaptic pruning may eliminate weaker synapses and retain stronger synapses.Synaptic pruning also plays a vital role in mediating the formation of neural circuit under physiological condition, contributes to cell and myelin debris clearance, promotes maturation of oligodendrocytes, which surround the bare axon to form myelin sheath, and helps the regeneration of neurons and synapses.Recently, increasing number of studies on microglial synaptic pruning has advanced our understanding of the underlying mechanism for synaptic pruning and its relevant physiological functions.Here, we reviewed microglial synaptic pruning function and its potential regulatory mechanisms in brain under physiological and pathological conditions.

Phenological characterization and temperature requirements of Annona squamosa L. in the Brazilian semiarid region

ABSTRACT The phenological growth stages of various species of monocotyledonous and dicotyledonous plants can be uniformly coded using the Biologische Bundesanstalt, Bundessortenamt, und Chemische Industrie (BBCH) scale. The aim of the present study was to determine the duration of different phenological stages and the temperature requirements of the sugar-apple, Annona squamosa, during two crop cycles in the semiarid region of Brazil. Phenological stages were divided into eight of ten possible principal stages: (0) bud development, (1) leaf development, (3) shoot/branch development, (5) inflorescence emergence, (6) flowering, (7) fruit development, (8) fruit maturity and (9) senescence and the beginning of dormancy. The phenological cycle of the sugar-apple from having closed leaf buds to the fruit ripening stage lasted 149 and 164 days with temperature requirements of 1684.5 and 1786.7 degree days (DD) for the first and second crop cycles, respectively. The results provided important information that will inform the correct timing for crop management practices.

Avaliações físico-quimicas de frutos de tomateiro em função de doses de potássio e nitrogenio / Evaluations physico-chemical in tomato in function of potassium and nitrogen doses

O objetivo deste trabalho foi estudar o efeito do nitrogênio e do potássio nas características físico-químicas de frutos de tomate cultivados em condições de campo. Utilizou-se um delineamento experimental em blocos ao acaso, em esquema fatorial 4 x 4, com quatro repetições: quatro doses de N (20,5, 41, 82 e 164 kg ha-1) obtidas respectivamente pela adição de 100, 200, 400 e 800 kg ha-1 de nitrocálcio e quatro doses de K2O (90, 180, 360 e 720 kg ha-1) obtidas respectivamente pela adição de 150, 300, 600 e 1200 kg ha-1 de cloreto de potássio. A cultivar de polinização aberta Santa Clara 5800 (Grupo Santa Cruz) foi cultivada no espaçamento 0,40 m x 0,40 m x 1,00 m em fileira dupla sendo a parcela total constituída por 24 plantas. Considerou-se como úteis as oito plantas centrais. As plantas foram podadas acima da terceira folha após o quarto ramo floral. Os teores de sólidos solúveis e totais, acidez total titulável e relação sólida solúveis/acidez não variaram em função dos tratamentos e apresentaram valores adequados para a cultura A variação no teor de açúcares redutores, pectina total, solubilização da pectina e vitamina C, embora significante, não foi importante sob o aspecto organoléptico, pós-colheita e nutricional, uma vez que respectivos valores ficaram dentro de uma faixa considerada adequada para o tomate. Os macronutrientes nitrogênio e potássio não alteraram as características físico-químicas do fruto do tomateiro

The aim of this work was to investigate the effect of increasing rates of nitrogen and potassium in qualitative characteristics of the tomato in field conditions. It was used an experimental design in randomized blocks in 4 x 4 factorial scheme with four replicates: four N rates (20.5, 41, 82 and 164 kg ha-1) obtained respectively by adding 100, 200 400 and 800 kg ha-1 nitrocalcium and four doses of K2O (90, 180, 360 and 720 kg ha-1) obtained respectively by adding 150, 300, 600 and 1200 kg ha-1 of potassium chloride. Cultivar Santa Clara 5800 open pollination was cultivated at the spacing 0,40 m x 0,40 m x 1,00 m, in double rows, the total plotes being comprised of 24 plants, of which the 8 central plants were used to collect data. The plants were pruned above the third leaf after the fourth floral branch. The contents of soluble solids, total solids, total titratable acidity and soluble solid/acidity ratio did not range significantly with the treatments and presented values within the acceptable ranges for tomato. The ranges in the content of reducing sugars, total solubilization pectin and vitamin C, although significant, were not important from the organoleptical, post-harvest and nutritional point of view, remaining within the normal ranges for tomato. The macronutrients nitrogen and potassium not altered the physical and chemical characteristics of tomato fruit

Chemical and technological evaluation of the Phyllanthus niruri aerial parts as a function of cultivation and harvesting conditions

Tea from Phyllanthus niruri L., Phyllanthaceae, aerial parts is commonly used by Brazilian folk medicine for its benefits on the treatment of genitourinary disorders, for what the polyphenolic compounds are mainly responsible. The yield of such compounds may be influenced by several variables related with the plant growth. The effects of planting periods and harvesting conditions are investigated in this work, including four different seasons. The cultivation was characterized by dry mass yield of aerial parts, and the effect of pruning was analyzed. Leaves, stems and their mixtures were analyzed after drying and milling. Loss on drying and water soluble extractives were used as physical parameters for quality control. Flavonoid content and gallic acid were chosen as chemical markers for this work. The spectrophotometric trial based on the aluminum chloride complexes was applied to evaluate the total flavonoids content. Gallic acid contents were measured from the water extractive solutions by high-performance liquid chromatography. The pruning caused a positive influence on the amount of leaves and stems. The highest flavonoids and gallic acid contents were found in the leaves, which were developed over the summer and the winter, respectively, both from the second harvesting (after pruning). Chomatographic profile by HPLC was characterizes by the presence of gallic acid and two other major peaks (not identified substances), which relation was peculiar to each aerial part. In conclusion, these results suggest that even under less favorable climatic conditions, in winter, the pruning seems to cause a strong influence over the P. niruri polyphenolics production. Indeed, the total flavonoids content, as well as the HPLC profile, can be used as indicative parameters of the ratio of leaves and stem in the vegetal raw material.

Effect analysis of Orthodontic traction and porcelain in the treatment of anterior teeth subgingival quit / 中国基层医药

Objective To investigate the effect of Orthodontic traction and porcelain in the treatment of anterior teeth subgingival quit.Methods 63 patients with anterior teeth subgingival quit were randomly assigned into observation group and control group,the control group were treated with conventional processing methods,the observation group were treated with orthodontic traction and porcelain repair nethod.the efficient and general treatment were compared.Results In the observation group,there were 12 cases cured,17 cases effective,2 cases ineffective,the total effective rate was 93.55％ (29/31) ; in the control group,there were 9 cases cured,14 cases effective,9 cases ineffctive,the total effective rate was 71.88％ (23/32).There were significantly difference between two groups (x2 =5.1323,P =0.0235).Tooth loose degrees,gingival condition and periodontal status and treatment of bone resorption rates of the observation group were 93.55％,93.55％,100.00％ and 96.77％,significantly higher than the control(71.88％,68.75％,71.88％,75.00％),the differences were statistically significant (all P ＜ 0.05).Crown retention between the two groups showed no significant difference between cases (P ＞ 0.05).Conclusion orthodontictraction has good effect in the treatment of former porcelain subgingival root pruning.

Fenologia e produção das videiras 'Alicante' e 'Syrah' em safra fora de época / Phenology and production of 'Alicante' and 'Syrah' grapevines grown out of season

O objetivo do trabalho foi caracterizar a fenologia e a produção das videiras 'Alicante' e 'Syrah' (Vitis vinifera L.) cultivadas fora de época. A área experimental foi instalada em uma propriedade da Vinícola Intervin®, localizada em Maringá, PR. Os vinhedos foram estabelecidos em julho de 2001 e as plantas conduzidas no sistema latada (4,0x1,5m), enxertadas sobre o porta-enxerto IAC 766 'Campinas'. As podas longas de produção (oito gemas) foram realizadas no fim de fevereiro de 2008 e 2009. As avaliações tiveram início a partir da poda de frutificação durante duas safras consecutivas fora de época. Foram utilizadas 20 plantas representativas de cada variedade, sendo a colheita de ambas as safras realizadas em meados de julho. Avaliou-se a duração em dias das principais fases fenológicas, as características físico-químicas das bagas e as características produtivas de cada variedade. Verificou-se que a 'Alicante' apresenta ciclo médio de 138,0 dias, mosto com 15,4°Brix, 1,9 por cento de ácido tartárico e produtividade de 14,5t ha-1, enquanto a 'Syrah' apresenta ciclo médio de 132,5 dias, 14,5°Brix, 2,9 por cento de ácido tartárico e produtividade de 11,4t ha-1.

This study aimed to characterize the phenology and the production of 'Alicante' and 'Syrah' grapes (Vitis vinifera L.) produced out of season. The experimental area was located in a property belonging to the Intervin® Winery, at Maringá, PR. The vineyards were established in July, 2001 and the vines were trained in an overhead trellising system (4.0x1.5m), grafted on IAC 766 'Campinas' rootstock. The long pruning of production (eight buds) was held in late February of 2008 and 2009. The evaluations started from the pruning of production, in two years, using 20 representative vines of each variety, and the harvesting of both crops were performed on early July. The phenology of the trees was evaluated as the duration in days of the most important phases, as well it was analyzed the berries physico-chemical characteristics. The production characteristics of each variety were also estimated. It was verified that 'Alicante' showed a mean cycle of 138.0 days, must with 15.4°Brix, 1.9 percent of tartaric acid and productivity of 14.5t ha-1, while 'Syrah' showed a mean cycle of 132.5 days, 14.5°Brix, 2.9 percent of tartaric acid and productivity of 11.4t ha-1.

Desfolha em videiras americanas e viníferas na fase de pré-maturação dos frutos / Defoliation of american and vinifera grapevines in the pre ripening of fruits

Foram avaliadas possíveis interferências que a localização da folha removida mediante desfolha em videira apresenta sobre o rendimento e qualidade dos frutos. As cultivares 'Niagara Branca' e 'Concord', ambas Vitis labrusca, e 'Cabernet Sauvignon' e 'Merlot', ambas Vitis vinifera, foram utilizadas em sistema de condução em espaldeira, na safra 2006/2007. O experimento foi conduzido em delineamento de blocos casualizados, com quatro repetições e quatro plantas por parcela, sendo testados 4 tratamentos: T1 (testemunha - sem desfolha), T2 (retirada das folhas localizadas opostas aos cachos), T3 (retirada das folhas do sarmento localizadas abaixo dos cachos) e T4 (retirada das folhas do sarmento localizadas abaixo e opostas aos cachos). As cultivares da espécie Vitis labrusca receberam um tratamento adicional T5, constando na retirada das folhas acima dos cachos. A aplicação dos tratamentos foi realizada na fase de mudança de cor das bagas para as cultivares 'Concord', 'Merlot' e 'Cabernet Sauvignon' e início do amolecimento das bagas para a cultivar 'Niagara Branca'. Avaliaram-se, após a colheita, a produção por planta, a massa dos cachos, os teores de sólidos solúveis (SS), a acidez titulável, (AT) e o pH dos frutos. Observou-se que a desfolha até a altura do cacho não influenciou na quantidade e na qualidade dos frutos. Porém, quando a desfolha foi realizada acima dos cachos, ocorreu um atraso na maturação das uvas americanas. Essa condição implica não ser necessária a desfolha seletiva até a altura dos cachos nas videiras estudadas, quando realizada na fase início de amadurecimento dos frutos.

This study aimed to evaluate the interference that the location of the leaf removed by defolation in grapevine has on fruits quality and yield. Such experiment was performed using Niagara Branca and Concord, both Vitis labrusca, and Cabernet Sauvignon e Merlot, both Vitis vinifera, trained in a unilateral cordon system during the 2006/2007 harvesting. The experiment was disposed in a randomized block design, with four replications and four plants per plot. The following treatments were tested: T1 (no defoliation), T2 (removal of leaves located in the opposite side of the grapes), T3 (removal of leaves located under the grapes) and T4 (removal of leaves located under and in the opposite side of the grapes). The Vitis labrusca received an additional treatment (T5) consisting of leaves removed above the grapes. These treatments were applied during the change of berries color for Concord, Cabernet Sauvignon and Merlot grapes and in the beginning of berries softening for Niagara Branca grape. After the harvesting, the grapevine production, cluster weight, total soluble solids, total titratable acidity and pH were evaluated. It was observed that the defoliation until the grapes height did not influence the quantity and quality of grapes. However, when the defoliation was carried out above the clusters, there was a delay in the maturation of american grapes. This condition shows that is not necessary a selective desfolation until the clusters height on grapevines, when it is realized during the initial fruit ripening phase.

Crescimento e produção de antúrio submetido a diferentes intensidades de desfolha / Growth and production of anthurium submitted to different defoliation intensities

O objetivo do presente trabalho foi avaliar o crescimento e a produção de Anthurium andraeanum 'Apalai' submetido a diferentes intensidades de desfolha no Vale do Ribeira, SP. Foram utilizadas mudas micropropagadas dessa espécie, as quais foram plantadas em canteiros construídos sob telado coberto com tela de náilon preta, com malha que proporciona 70 por cento de sombreamento, no espaçamento 0,40x0,40m. O experimento foi conduzido em delineamento de blocos casualizados em parcelas subdivididas, com dezesseis repetições. O efeito da desfolha foi avaliado na parcela e o efeito do tempo após a desfolha na subparcela. As diferentes intensidades de desfolha consistiram em plantas com três, quatro e cinco folhas e plantas sem desfolha, e o tempo após a desfolha, as avaliações realizadas em 2006 e 2007. Ocorreu um aumento na área das folhas individuais em plantas mantidas com quatro e cinco folhas, proporcional à intensidade de desfolha, que pode ser relacionado a uma tentativa de compensação da área foliar perdida. A desfolha com a manutenção de três, quatro ou cinco folhas em plantas de antúrio 'Apalai' é prejudicial para o seu crescimento e produção.

The aim of this research was to evaluate the growth and yield of Anthurium andraeanum 'Apalai' submitted to different defoliation intensities in Vale do Ribeira, SP. In order to develop this research, micropropagated seedlings of this species were used, planted in beds built under a roof covered with a black nylon screen, with a mesh that provides 70 percent shading, spaced 0.40x0.40m apart. The experiment was conducted in a randomized block design in split splot with sixteen replicates.The defoliation effect was evaluated in the whole plots and the effect of time after defoliation was evaluated in the split-plots. The different defoliation intensities consisted of plants with three, four and five leaves and plants without defoliation, and the time after defoliation. Assessments were conducted in 2006 and 2007. There was an increase in the area of individual leaves in plants maintained with four and five leaves, proportional to the defoliation intensity, which may be related to an attempt to offset the lost leaf area. Defoliation caused by the maintenance of three, four or five leaves in anthurium 'Apalai' plants is detrimental to their growth and production.

Sistema desponte na produção de figos verdes 'Roxo de Valinhos' / Lopping system in the production of 'Roxo de Valinhos' green fig

O objetivo deste trabalho foi estudar o efeito do sistema desponte sobre o desenvolvimento e a produção de figos verdes 'Roxo de Valinhos'. O experimento utilizando plantas de quatro anos de idade, com espaçamento 3 x 2m, foi conduzido de julho de 2007 a março de 2008, em Quatro Pontes, Paraná (PR). O delineamento utilizado foi em blocos ao acaso, com quatro blocos, e os tratamentos foram arranjados em fatorial 2 x 5, tomando por fatores o número de ramos produtivos (plantas conduzidas com seis ou 12 ramos) e o número de despontes (um, dois, três ou quatro, além do controle sem desponte). No sistema desponte, após a emissão da 16a folha, o ramo foi despontado (gema apical removida), selecionando-se duas brotações por ramo produtivo. Novos despontes foram realizados posteriormente, sempre após a emissão da sexta folha. Em cada parcela, constituída de três plantas úteis, foram coletados dados no ciclo de produção 2007/08. A maior produção (2.208,87g planta-1) e produtividade estimada (3.681,19kg ha-1) observada de figos verdes foram obtidas quando as plantas foram conduzidas com 12 ramos produtivos, efetuando-se três despontes.

The objective of this research was to study the effect of the lopping system on the development and production of green figs 'Roxo de Valinhos'. The experiment using plants of four years of age, with spaced 3 x 2m, was conducted from July/2007 to March/2008 in Quatro Pontes, PR. The design was randomized blocks with four blocks, and the treatments arranged in a 2 x 5, taking as factors the number of productive branches (plants conducted with six or twelve branches) and the number of loppings (one, two, three or four, besides the control without lopping). In the lopping system, after the emission of the 16th leaf, the branch was blunted (apical bud removed), selecting two sprouting per productive branches. New lopping was accomplished later, always after the emission of sixth leaf. In each plot, consisting of the three plants, data were collected in the production cycles 2007/2008. The highest yield (2,208.87g planta-1) and estimated yield (3,681.19kg ha-1) observed of green figs were obtained when plants were conducted with twelve branches of production, effecting three loopings.

Teores de carboidratos, proteínas e aminoácidos livres durante o desenvolvimento de raízes tuberosas do feijão macuco (Pachyrrhizus tuberosus (Lam.) Spreng.), em área de várzea, Amazonas, Brasil

Among the species of plants that produce tuberous starched roots in the tropical region, Pachyrrhizus tuberosus, leguminous plant, which is popularly named feijão macuco, jacatupé or yam bean. This study proposed to identify some physiological aspects of the yam bean cultivated in foodplain areas (várzeas). Three different genetic materials (2.2, 1.2 and 1.1.6) studied by the breeding program of National Institute for Amazonian Research (INPA) (numbered 1, 2 and 3), were submitted to two different kinds of cultivation methods: with and without tutoring and with and without inflorescence pruning. Inflorescence pruning improved the xilose, glicose, reduced sugars, protein and aminoacid contents. The genetic materials did not present differences in terms of plant development, but some variations of xilose, reduced sugars and protein contents were observed among them. The starch content in the roots did not present variation during the plant growth under any of the treatments studied.

Entre as espécies que produzem tubérculos amiláceos nas condições tropicais, encontra-se Pachyrrhizus tuberosus, leguminosa conhecida popularmente como feijão macuco ou jacatupé. Propôs-se estudar alguns aspectos fisiológicos do desenvolvimento dessa espécie em uma area de várzea. Foi estudado o comportamento de três introduções (2.2, 1.2 e 1.1.6) da coleção do programa de melhoramento de hortaliças do Instituto Nacional de Pesquisas da Amazônia - INPA (definidas como 1, 2 e 3), submetidas a duas formas de manejo: com e sem tutoramento das plantas e com e sem poda das inflorescências. A poda das inflorescências resultou em um aumento nos teores de xilose, glicose, açúcares redutores, proteínas e aminoácidos ao longo do desenvolvimento. Foram observadas variações nos teores de xilose, açúcares redutores e proteínas entre as introduções estudadas. O teor de amido nas raízes tuberosas não mostrou variação ao longo do desenvolvimento, em nenhum dos tratamentos estudados.