Intervenção Breve para uso de Substâncias Psicoativas no Brasil: revisão sistemática / Brief Intervention for Psychoactive Substance use in Brazil: a systematic review

RESUMO As Intervenções Breves têm sido preconizadas há mais de 20 anos pela Organização Mundial da Saúde como uma estratégia de prevenção aos problemas relacionados ao uso de Substâncias Psicoativas, tendo como prioridade a aplicação nos serviços de atenção primária à saúde visando a diminuição dos riscos relacionados a esse fenômeno. Diante disso, o objetivo do presente estudo foi mapear e descrever as evidências científicas existentes na literatura sobre o uso de Intervenção Breve para o uso de Substâncias Psicoativas desenvolvidas no Brasil. Trata-se de revisão sistemática. As buscas foram realizadas em sete fontes de informações, em agosto de 2021. Todos os estudos foram desenvolvidos com abordagem quantitativa. O álcool esteve presente na maioria dos estudos, seguido da maconha e nicotina. O cenário predominante foi a unidade básica de saúde, com público de adultos de ambos os sexos e universitários. A maioria apresentou como proposta a Intervenção Breve grupal presencial e somente dez indicaram o referencial norteador da Intervenção Breve utilizada. No Brasil, as Intervenções Breves são incipientes e os estudos apresentados não destacam com exatidão o referencial norteador da intervenção utilizada.

ABSTRACT Brief Interventions have been recommended for more than 20 years by the World Health Organization as a strategy to prevent problems related to the use of Psychoactive Substances, with priority being applied in primary health care services in order to reduce the risks related to this substance. phenomenon. Therefore, the objective of the present study was to map and describe the existing scientific evidence in the literature on the use of Brief Intervention for the use of Psychoactive Substances developed in Brazil. This is a systematic review. Searches were carried out in seven sources of information in August 2021. All studies were developed with a quantitative approach. Alcohol was present in most studies, followed by marijuana and nicotine. The predominant scenario was the basic health unit, with a public of adults of both sexes and university students. The majority presented a brief face-to-face group intervention as a proposal and only ten indicated the guiding reference of the Brief Intervention used. In Brazil, Brief Interventions are incipient and the studies presented do not accurately highlight the guiding reference for the intervention used.

Riesgo de consumo de sustancias psicoactivas y su relación con el uso problemático de videojuegos y redes sociales en estudiantes universitarios

Introducción: El nivel de riesgo de consumo se establece a partir de la exposición que tiene una persona a ciertos factores de riesgo o características individuales, familiares o sociales, que posibilitan o aumentan el consumo de sustancias psicoactivas (SPA). Objetivo: Este estudio tuvo como propósito relacionar el nivel de riesgo de consumo de SPA con el uso problemático de videojuegos y redes sociales, a fin de establecer su posible papel como factores de riesgo de tipo social. Método: Se realizó un estudio de tipo cuantitativo con un diseño no experimental transversal de alcance descriptivo correlacional con la participación de 736 estudiantes. Se emplearon como instrumentos de recolección de datos el cuestionario sociodemográfico, prueba de tamizaje ASSIST 3,1, cuestionario de adicción a redes sociales - ARS y el cuestionario de Experiencias Relacionadas con los Videojuegos - CERV. Resultados: Algunos resultados sugieren mayor exposición al consumo de sustancias alcohólicas, derivados del tabaco, del cannabis y mayores niveles en el uso problemático de redes sociales y videojuegos. Conclusiones: A partir de lo hallado, las evidencias apuntan a que el aumento de riesgo no depende de la tipología familiar, al contrario de lo que pasa con el sexo, a su vez el consumo de tabaco, alcohol, cocaína, inhalantes, sedantes y opiáceos dependen de variaciones.

Introduction: The level of risk of consumption is established from the exposure that a person has to certain risk factors or individual, family, or social characteristics, which enable or increase the consumption of psychoactive substances (PAS). Objective: The purpose of this study was to relate the level of risk of PAS consumption with the problematic use of video games and social networks, in order to establish their possible role as social risk factors. Method: A quantitative study was carried out with a non-experimental cross-sectional design of descriptive correlational scope with the participation of 736 students. The sociodemographic questionnaire, ASSIST 3,1 screening test, social network addiction questionnaire ARS and the Video Game Related Experiences Questionnaire CERV were used as data collection instruments. Results: It was found Some results suggest greater exposure to the consumption of alcoholic substances, tobacco derivatives, cannabis derivatives and higher levels in the problematic use of social networks and video games. Conclusions: From what was found, the evidence points to the fact that increased risk does not depend on family typology, contrary to what happens with sex, in turn the consumption of tobacco, alcohol, cocaine, inhalants, sedatives and opiates depend on variations.

Analysis of Forty-Two Psychoactive Substances in a Single Hair by Micro-Segmental Technique / 法医学杂志

OBJECTIVES@#To establish an LC-MS/MS method based on single hair micro-segmental technique, and verify the detection of 42 psychoactive substances in 0.4 mm hair segments.@*METHODS@#Each piece of single hair was cut into 0.4 mm segments and extracted by sonication and the segments were immersed in dithiothreitol-containing extraction medium. Mobile phase A was the aqueous solution containing 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile. Mobile phase B was acetonitrile. An electrospray ionization source in positive ion mode was used for data acquisition in multiple reaction monitoring (MRM) mode.@*RESULTS@#The 42 psychoactive substances in hair had a good linear relationship within their respective linear ranges (r>0.99), the limits of detection were 0.2-10 pg/mm, the limits of quantification were 0.5-20 pg/mm, the intra-day and inter-day precisions were 1.5%-12.7%, the intra-day and inter-day accuracies were 86.5%-109.2%, the recovery rates were 68.1%-98.2%, and the matrix effects were 71.3%-111.7%. The method was applied to hair samples collected from one volunteer at 28 d after a single dose of zolpidem, with zolpidem detected in 5 hairs was 1.08-1.60 cm near the root tip, and the concentration range was 0.62-20.5 pg/mm.@*CONCLUSIONS@#The micro-segmental technique of single hair analysis can be applied to the investigation of drug-facilitated sexual assault cases.

Structural Analysis and Characterization of 4-F-α-PVP Analog 4-F-3-Methyl-α- PVP Hydrochloride / 法医学杂志

OBJECTIVES@#To identify 1-(4-fluorophenyl)-2-(1-pyrrolidinyl) pentan-1-one (4-F-α-PVP) analog 1-(4-fluoro-3-methyl phenyl)-2-(1-pyrrolidinyl) pentan-1-one (4-F-3-Methyl-α-PVP) hydrochloride without reference substance.@*METHODS@#The direct-injection electron ionization-mass spectrometry (EI-MS), GC-MS, electrospray ionization-high resolution mass spectrometry (ESI-HRMS), ultra-high performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS/MS), nuclear magnetic resonance (NMR), ion chromatography and Fourier transform infrared spectroscopy (FTIR) were integrated utilized to achieve the structural analysis and characterization of the unknown compound in the sample, and the cleavage mechanism of the fragment ions was deduced by EI-MS and UPLC-HRMS/MS.@*RESULTS@#By analyzing the direct-injection EI-MS, GC-MS, ESI-HRMS and UPLC-HRMS/MS of the compound in the samples, it was concluded that the unknown compound was a structural analog of 4-F-α-PVP, possibly with one more methyl group in the benzene ring. According to the analysis results of 1H-NMR and 13C-NMR, it was further proved that the methyl group is located at the 3-position of the benzene ring. Since the actual number of hydrogen in 1H-NMR analysis was one more than 4-F-3-Methyl-α-PVP neutral molecule, it was inferred that the compound existed in the form of salt. Ion chromatography analysis results showed that the compound contained chlorine anion (content 11.14%-11.16%), with the structural analysis of main functional group information by FTIR, the unknown compound was finally determined to be 4-F-3-Methyl-α-PVP hydrochloride.@*CONCLUSIONS@#A comprehensive method using EI-MS, GC-MS, ESI-HRMS, UPLC-HRMS/MS, NMR, ion chromatography and FTIR to identify 4-F-3-Methyl-α-PVP hydrochloride in samples is established, which will be helpful for the forensic science laboratory to identify this compound or other analog compounds.

Methcathinone Increases Visually-evoked Neuronal Activity and Enhances Sensory Processing Efficiency in Mice / 神经科学通报·英文版

Methcathinone (MCAT) belongs to the designer drugs called synthetic cathinones, which are abused worldwide for recreational purposes. It has strong stimulant effects, including enhanced euphoria, sensation, alertness, and empathy. However, little is known about how MCAT modulates neuronal activity in vivo. Here, we evaluated the effect of MCAT on neuronal activity with a series of functional approaches. C-Fos immunostaining showed that MCAT increased the number of activated neurons by 6-fold, especially in sensory and motor cortices, striatum, and midbrain motor nuclei. In vivo single-unit recording and two-photon Ca2+ imaging revealed that a large proportion of neurons increased spiking activity upon MCAT administration. Notably, MCAT induced a strong de-correlation of population activity and increased trial-to-trial reliability, specifically during a natural movie stimulus. It improved the information-processing efficiency by enhancing the single-neuron coding capacity, suggesting a cortical network mechanism of the enhanced perception produced by psychoactive stimulants.

The role of spirituality and religiosity in psychoactive substance use among adolescents in a Nigerian Hospital

Objective: Adolescence is a transition period where drug experimentation is common. Spirituality and religiosity have been recognized by literature as having a possible role in stabilizing good physical and mental health. To compare the religiosity and spirituality among adolescents abusing a psychoactive substance and those not abusing psychoactive substances in a Nigerian hospital Methods: This was a cross-sectional descriptive study conducted among adolescent patients admitted to the drug rehabilitation unit and adolescents attending the General Outpatient Department of the ABUAD Multisystem Hospital, Ado-Ekiti. The Daily Spiritual Experience Scale and Centrality of Religiosity Scale were used Results: The prevalence of high religiosity and spirituality among the respondents was 57.9% and 56.1%. The prevalence of high religiosity and spirituality among respondents not abusing psychoactive substances was 62.9% and 62.6% respectively, while the level of high spirituality and religiosity among respondents abusing psychoactive substances was 53.0% and 49.1% respectively. A significant proportion of the respondents who had a low level of religiosity (55.9%) abused psychoactive substances than respondents who do not abuse psychoactive substances (45.7%) (X2=7.694 p=0.006). Similarly, more proportion of respondents who had a low level of spirituality (57.4%) abused psychoactive substances than respondents who do not abuse psychoactive substances (44.2%). The observation is statistically significant (X2=11.1, p<0.001). In addition, there was a significant positive correlation between the spirituality and religiosity scores among the respondents (r =0.50, p<0.001) Conclusion: Psychoactive drug use among the respondent was associated with both low religiosity and spirituality. Therefore, religious activities should be encouraged among adolescents.

Identification of the New Psychoactive Substance Eutylone / 法医学杂志

OBJECTIVES@#To establish a method to identify unknown sample based on the combined use of Fourier transform infrared spectroscopy (FTIR), gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF-MS), ultra-high performance liquid chromatography-linear ion trap quadrupole-orbitrap mass spectrometry (UPLC-LTQ-Orbitrap MS) and 1H-nuclear magnetic resonance spectroscopy (1H-NMR) technique.@*METHODS@#The unknown sample was directly analyzed by FTIR. The unknown sample was dissolved in methanol solution containing internal standard SKF525A and the supernatant was detected by GC-QTOF-MS and UPLC-LTQ-Orbitrap MS. The unknown sample was dissolved in methanol-d4 solution for structural analysis of 1H-NMR.@*RESULTS@#The characteristic absorption peaks of FTIR spectra obtained from unknown sample were 1 682 (C=O bond), 1 503, 1 488, 1 436, 1 363, 1 256, 1 092, 1 035, 935, 840 and 800 cm-1, the characteristic fragment ions (m/z) of GC-QTOF-MS were 86.096 4 (base peak), 58.065 1, 149.023 5, 121.028 6 and 65.038 6, the accurate mass [M+H]+ detected by UPLC-LTQ-Orbitrap MS was 236.127 7. The sample was identified as synthetic cathinone new psychoactive substance Eutylone by 1H-NMR.@*CONCLUSIONS@#The method established in this study can be used for structural confirmation of Eutylone.

Emociones, creencias y actitudes del personal asistencial hacia habitantes de calle que usan drogas en Medellín, Colombia / Emotions, beliefs and attitudes of healthcare workers towards homeless who use drugs in Medellín, Colombia / Emoções, crenças e atitudes do pessoal de assistência social com os moradores de rua que usam drogas em Medellín, Colômbia

Resumen Objetivo: Identificar algunos aspectos relacionados con el estigma de personal asistencial hacia habitantes de calle consumidores de sustancias psicoactivas. Metodología: Para lograr este objetivo, se llevó a cabo un estudio cualitativo, con enfoque hermenéutico y de alcance descriptivo, mediante el análisis categorial de las transcripciones de las grabaciones de los discursos de dos grupos focales con 32 participantes, miembros del Sistema de Atención al Habitante de Calle Adulto de Medellín, Colombia. La sistematización y la codificación del análisis se hicieron en un archivo de Excel®, destacándose la emergencia de las categorías: "emociones", "creencias" y "actitudes". Resultados: En los discursos de los participantes, se reiteran contenidos ambivalentes de los estigmas hacia esta población. Por una parte, la frustración en su trabajo, como principal emoción que coincide con las creencias de desconfianza y actitudes evasivas hacia ellos; y, por otro, los sentimientos de gratificación por la labor realizada, acordes con posturas más protectoras y pacientes durante el acompañamiento, pero que no se alejan de un ideal moralista de cero consumos. Conclusión: Reconocer y discutir abiertamente sobre el estigma podría orientar otras formas más humanizadas en la labor con este tipo de población, en tanto se devela que el rol profesional no nos exime del prejuicio, el estereotipo y la discriminación.

Abstract Objective: To identify some aspects related to the stigma of healthcare workers towards the homeless who consume psychoactive substances. Methodology: To achieve this objective, a qualitative study with a hermeneutic approach and descriptive scope was carried out, through the categorical analysis of transcriptions of recorded speeches from two focus groups comprised of 32 participants, members of the System of Attention to the Homeless Adult in Medellín, Colombia. Systematization and analysis encoding were performed in an Excel ® file, highlighting the presence of categories: "emotions", "beliefs" and "attitudes". Results: Ambivalent contents of the stigma towards this population are frequent in participant's speeches. On the one hand, the frustration about healthcare work, as the main emotion that coincides with the beliefs of distrust and evasive attitudes towards the homeless. On the other hand, the feelings of gratification for the work done and the presence of more protective and forbearing attitudes, but that do not alienate from the moralistic ideal of zero consumption. Conclusion: Recognizing and openly discussing stigma could guide more humanized ways of working with this type of population, as it is showed that the healthcare role does not exempt them from prejudice, stereotype and discrimination.

Resumo Objetivo: Identificar alguns aspectos relacionados ao estigma do pessoal de assistência social com os moradores de rua consumidores de substâncias psicoativas. Metodologia: Para alcançar este objetivo, foi realizado um estudo qualitativo, com abordagem hermenêutica e âmbito descritivo, através da análise categorial das transcrições das gravações dos discursos de dois grupos focais com 32 participantes, membros do Sistema de Atenção ao Morador de Rua Adulto de Medellín, Colômbia. A sistematização e a codificação da análise foram feitas num arquivo Excel®, destacando-se a emergência das categorias: "emoções", "crenças" e "atitudes". Resultados: Os participantes reiteraram em suas falas, conteúdos ambivalentes dos estigmas dessa população. Por um lado, a frustração em seu trabalho, com emoção principal que coincide com as crenças de desconfiança e atitudes evasivas com eles; e por outro, os sentimentos de gratidão pelo trabalho realizado, de acordo com posturas mais protetoras e pacientes durante o acompanhamento, mas que não se distanciam de um ideal moralista de consumo zero. Conclusão: Reconhecer e discutir abertamente o estigma poderia orientar outras formas mais humanizadas no trabalho com este tipo de população, e ao mesmo tempo se revela que o compromisso profissional não os exime do preconceito, o estereótipo e a discriminação.

Personality and coping styles in medical internees: a cross-sectional study

Background and aims: Medical education is an elaborate course requiring knowledge assimilation, skill development, and academic perfection. Emerging out of the course successfully is a strenuous process which tests students’ psychological domains like personality, coping skills, and so on. The present study was planned with this primary objective of assessing the personality traits, psychoactive-substance use, and coping skills in the medical students and the secondary objective of finding the impact of personality and psychoactive-substance use on coping styles. Methods: The study was conducted on internees, recruited on individual approach over a period of one year. Ethical clearance was taken. Consenting candidates were administered sociodemographic proforma, the Eysenck Personality Inventory, and the coping checklist. Pearson’s correlation and independent t-test were used for analysis. Results: A total of 167 internees took part in study. On personality assessment, mean score of extraversion was 12.56 (±3.78) suggesting ambiversion and neuroticism was 9.36 (±4.89) suggesting mixed neurotic traits. Thirty four (20%) used psychoactive-substance. Problem-solving, distraction-positive, and acceptance-redefinition were frequently used coping styles. Extraversion correlated positively with distraction-negative (p<0.01) and negatively with acceptance-redefinition (p=0.03). Neuroticism had significant positive correlation with problem-solving (p=0.03), distraction-positive (p<0.01), distraction-negative (p<0.01), faith-religion coping (p<0.001), and blame-denial coping (p<0.001). On gender-wise assessment, males used more of distraction-negative (p=0.02) and females used more of faith-religion coping styles (p=0.02). Psychoactive-substance users elaborated more of distraction-negative and less of acceptance-redefinition coping. Conclusion: Internees had ambiversion and mixed neurotic personality traits. Their personality and substance use had significant influence on the types of coping styles used. There is need of training to effectively manage stress using appropriate coping strategies based on their personality.

Educação em saúde para a qualidade de vida de usuários de substâncias psicoativas / Educación en salud para la calidad de vida de los usuarios de sustancias psicoactivas / Health education for the quality of life of users of psychoactive substances

Objetivo comparar os impactos de duas estratégias educativas em saúde na qualidade de vida de pessoas com transtornos por uso de substâncias psicoativas. Método estudo descritivo, analítico e experimental realizado com 40 usuários de um Centro de Atenção Psicossocial Álcool e Outras Drogas. Foi aplicado um questionário para comparação da adesão ao tratamento e os problemas relatados pelos usuários em tratamento, antes e após a aplicação de duas estratégias educativas. As variáveis estudadas foram a adesão em relação ao tratamento e a frequência dos problemas nos âmbitos financeiro, social e de saúde. Resultados no grupo com intervenções dialógicas, houve maior pontuação na adesão e melhor ganho no aspecto econômico, familiar e social em comparação ao grupo com estratégia tradicional. Não houve diferenças no âmbito da saúde. Conclusão a estratégia educativa dialógica demonstrou ser mais efetiva na resolução de alguns problemas relatados por usuários de substâncias psicoativas.

Objetivo comparar los impactos de dos estrategias educativas en la salud en la calidad de vida de las personas con trastornos del consumo de sustancias psicoactivas. Método estudio descriptivo, analítico y experimental realizado con 40 usuarios de un Centro de Atención Psicosocial en Alcohol y Otras Drogas. Se aplicó un cuestionario para comparar el cumplimiento del tratamiento y los problemas reportados por los usuarios sometidos a tratamiento, antes y después de la aplicación de dos estrategias educativas. Las variables estudiadas fueron la adhesión al tratamiento y la frecuencia de los problemas en los ámbitos financiero, social y de salud. Resultados en el grupo con intervenciones con diálogos, hubo una puntuación más alta en la participación y una mejor ganancia en los aspectos económicos, familiares y sociales en comparación con el grupo con la estrategia tradicional. No hubo diferencias en la salud. Conclusión la estrategia educativa con diálogos demostró ser más eficaz en la resolución de algunos problemas reportados por los usuarios de sustancias psicoactivas.

Objective to compare the impacts of two health educational strategies on the quality of life of people with psychoactive substance use disorders. Method descriptive, analytical and experimental study conducted with 40 users of a Psychosocial Care Center for Alcohol and Other Drugs. A questionnaire was applied to compare treatment adherence and the problems reported by users undergoing treatment, before and after the application of two educational strategies. The variables studied were treatment adherence and frequency of problems in the financial, social and health spheres. Results in the group with dialogical interventions, there was a higher score on the participation and better gain in the economic, family and social aspects compared to the group with traditional strategy. There were no differences in health. Conclusion the dialogical educational strategy proved to be more effective in solving some problems reported by users of psychoactive substances.

Research Progress on the Effect of Synthetic Cathinones on Animal Behavior / 法医学杂志

Synthetic cathinones are a class of new psychoactive substances with a structure similar to amphetamine drugs, which can produce excitatory effects similar to drugs such as amphetamine and cocaine after being taken. In recent years, the abuse of synthetic cathinones worldwide has become increasingly serious, posing a serious threat to social security and public health. This article focuses on several common synthetic cathinones, collects their research results in animal autonomous activity experiments and drug dependence model experiments and summarizes their relevant experimental conclusions in animal body temperature regulation, learning and memory, and anxiety, in order to provide data reference and method guidance for the domestic development of related drug research.

Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS / 法医学杂志

Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for rapid screening and testing of three types of new psychoactive tryptamines in human blood： 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT. Methods The effects of the type of extractant, the type and dosage of salting-out agent, and the dosage of adsorbent on the test results of the three tryptamines were investigated. Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS. Results The linear relationships of 5-MeO-DALT, 5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100, 0.5-100 and 0.2-100 ng/mL, respectively, with their coefficients higher than 0.99. The limits of detection （LODs） were 0.1-0.2 ng/mg. The recoveries ranged from 84.86% to 94.57%. Intra-day and inter-day precisions were good. Conclusion The method is simple, rapid, easy to operate and has a high recovery. It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.

Identification of New Psychoactive Tryptamines 4-OH-MET and 4-AcO-DMT Using High Resolution Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy / 法医学杂志

Objective To detect the uncontrolled new psychoactive tryptamines involved in drug-related cases with high resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Methods White and brown powder obtained in actual cases were extracted and analyzed by gas chromatography-quadrupole time-of-flight mass spectrometry （GC-QTOF-MS）, ultra-high performance liquid chromatography-linear ion trap quadrupole-orbitrap mass spectrometry （UPLC-LTQ-Orbitrap MS） and 1H-nuclear magnetic resonance spectroscopy （1H-NMR）. Results After detection by GC-QTOF-MS, the components of white powder showed main characteristic fragment ion peaks at m/z 218.141 0 （molecular ion peak）, 72.080 6 （base peak）, etc. After detection by UPLC-LTQ-Orbitrap MS, its protonated molecular ion was m/z 219.149 4. The main ions in the secondary mass spectrum under the collision-induced dissociation （CID） mode were m/z 160.076 3 and 72.080 8. After detection by GC-QTOF-MS, the components of brown powder showed main characteristic fragment ion peaks at m/z 246.135 7 （molecular ion peak）, 58.065 1 （base peak）, etc. After detection by UPLC-LTQ-Orbitrap MS, its protonated molecular ion was m/z 247.145 0. The main ions in the secondary mass spectrum under CID mode were m/z 202.087 1, 160.076 3 and 134.060 5. NIST 17 library retrieval and 1H-NMR confirmed that the white powder and brown powder contained new psychoactive tryptamines 4-OH-MET and 4-AcO-DMT, respectively. Conclusion GC-QTOF-MS, UPLC-LTQ-Orbitrap MS and 1H-NMR can be used together to identify unknown new psychoactive substances.

Identification of Synthetic Cannabinoid New Psychoactive Substances 4F-MDMB-BUTINACA and MDMB-4en-PINACA / 法医学杂志

Objective To establish a method that combines a series of techniques including Fourier transform infrared spectrum （FTIR）, gas chromatography-mass spectrometry （GC-MS）, high resolution mass spectrometry and nuclear magnetic resonance spectroscopy （NMR） for identification of unknown substances. Methods The unknown samples （off-white powder and yellow crystal） seized in the actual cases were detected by FTIR, GC-MS （methanol as solvent）, high resolution mass spectrometry （methanol as solvent） and NMR （deuterated methanol as solvent）. Results The mass spectrum characteristic ions m/z of the main components in the samples measured by GC-MS were 219 （base peak）, 363, 307, 304, 275, 145, 131 and 213 （base peak）, 357, 301, 298, 269, 185, 171, 145 and 131, respectively. The accurate mass numbers [M+H]+ measured by high resolution mass spectrometry were 364.203 61 and 358.212 34, respectively. The unknown samples were identified as synthetic cannabinoid new psychoactive substances 4F-MDMB-BUTINACA and MDMB-4en-PINACA after data consultation and database retrieval and comparison, combined with infrared analysis and mass spectrometry data analysis, and their structures were confirmed by 1H-NMR. Conclusion The established multi-technology joint identification method can be used to identify 4F-MDMB-BUTINACA and MDMB-4en-PINACA in unknown samples. This method is fast, convenient, accurate, reliable and practical, and can provide reference for the identification of cases involving such substances in the future.

Determination of Salt Forms of New Psychoactive Substances by Ion Chromatography / 法医学杂志

Objective To establish an ion chromatography method for the salt form determination of new psychoactive substances （NPS）. Methods The method of conducting qualitative and quantitative analysis of six types of organic acid ions （acetate ion, tartrate ion, maleate ion, oxalate ion, fumarate ion, citrate ion） and five types of inorganic anions （fluoride ion, chloride ion, nitrate ion, sulfate ion, phosphate ion） in NPS sample by ion chromatography was developed. The salt forms of 222 seized NPS samples （103 samples with synthetic cannabinoids, 81 samples with cathinones, 44 samples with phenylethylamines, 12 samples with tryptamines, 7 samples with phencyclidines, 6 samples with piperazines, 2 samples with aminoindenes, 26 samples with fentanyls and 43 samples with other types of NPS） were analyzed by this method. Results Each anion had good linearity in the corresponding linear range, the correlation coefficients （r） were greater than 0.999, the limits of detection were 0.01-0.05 mg/L, and the limits of quantitative were 0.1-0.5 mg/L. Except that 5F-BEPIRAPIM was hydrochloride, the salt forms of the other 102 synthetic cannabinoids were all base. The salt form of 81 cathinone samples, 44 phenylethylamine samples, 7 phencyclidine samples and 2 aminoindene samples were all hydrochloride. The salt forms of tryptamine samples included base, hydrochloride, fumarate and oxalate. The salt forms of piperazine samples included base and hydrochloride. The salt forms of fentanyl samples and samples of other types included base, hydrochloride and citrate. Conclusion Ion chromatography is a simple, accurate and efficient method for determining the salt form of NPS samples, which makes the qualitative and quantitative conclusions of NPS more scientific and rigorous.

Identification of Synthetic Cannabinoid 5F-EDMB-PICA in Suspicious Herbal Products / 法医学杂志

Objective To study the qualitative analysis strategy for unknown synthetic cannabinoid in the suspicious herbal product when no reference substance is available. Methods The synthetic cannabinoid in herbal blend was extracted with methanol. The extract was concentrated by rotary evaporator and separated and purified by preparative liquid chromatography, to obtain high purity synthetic cannabinoid sample. Gas chromatography-mass spectrometry （GC-MS）, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-QTOF-MS） and nuclear magnetic resonance （NMR） were used to determine the structure of the prepared compound. Results High purity unknown sample （10 mg） was obtained by preparative liquid chromatography. The sample was analyzed by GC-MS, UPLC-TOF-MS and NMR, and through spectrum analysis, the unknown synthetic cannabinoid was determined as 5F-EDMB-PICA. Conclusion The method to extract unknown synthetic cannabinoid from low content herbal products by preparative liquid chromatography was established, and the structure of the unknown sample was identified by comprehensive use of GC-MS, UPLC-QTOF-MS and NMR. The information will assist forensic laboratories in identifying this substance or other compounds with similar structures in their casework.

Analysis and Application of Seven Common New Psychoactive Substances of Synthetic Cannabinoids in Hair / 法医学杂志

Objective To establish a detection method for common new psychoactive substances of synthetic cannabinoids in hair with ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods In the 1 mL of internal standard methanol solution, 20 mg hair was added. After cryogenic grinding and ultrasonic extraction, the extract was separated by ACQUITY UPLC HSS T3 column （100 mm×2.1 mm, 1.8 μm）. The mobile phase A was aqueous solution that composed of 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile. The mobile phase B was acetonitrile. Electrospray ionization source in positive ion mode was used for data acquisition in multi-reaction monitoring （MRM） mode. Results The seven common new psychoactive substances of synthetic cannabinoids in hair had a good linear relationship within their respective linear ranges （r>0.99）, the limits of detection were 0.5-2 pg/mg, the limits of quantification were 1-5 pg/mg, the intra-day and inter-day precisions were 0.1%-12.6%, the intra-day and inter-day accuracies were 89.2%-110.7%, the recovery rates were 52.3%-93.3%, and the matrix effects were 19.1%-95.2%. Conclusion The established method has a simple sample preparation process and high sensitivity. It is suitable for qualitative and quantitative analysis of common new psychoactive substances of synthetic cannabinoids in hair.

Research Advances in the Monitoring of New Psychoactive Substances in Municipal Wastewater / 法医学杂志

In recent years, as the third-generation of drugs, new psychoactive substances （NPS） have expanded rapidly and become a serious concern for China's anti-drug prevention and control system. As a new drug monitoring technology in the current anti-drug field, wastewater analysis is an objective, real-time, accurate, convenient and effective drug monitoring method. In recent years, it has gradually been applied to the monitoring of NPS. This study summarizes wastewater sample collection, target stability research, wastewater sample pretreatment, wastewater sample analysis methods, target NPS consumption calculations and actual monitoring applications, with a view to the construction of a monitoring system for NPS in wastewater, real-time and accurate grasp of information on the use of NPS in cities, the reflection of the actual consumption of different types of NPS and consumption trends in a short period of time, and prediction of the development trend of abused use, which is of great significance for combating NPS crimes, serving and guaranteeing the personal safety of the people, and maintaining social stability.

Research Progress on Metabolite Identification of Synthetic Cannabinoid New Psychoactive Substances / 法医学杂志

Synthetic cannabinoids are currently a class of new psychoactive substances with the largest variety and most abused. Metabolite identification research can provide basic data for monitoring synthetic cannabinoids abuse, which is the current research hotspot. The main trend of structural modification of synthetic cannabinoid is to replace the fluorine atom on pentyl indole or indazole cyclopentyl with hydrogen atom, which greatly improves the biological activity of the compound. The main metabolic reactions include hydroxylation, fluoropentyl oxidative, ester hydrolyze, amide hydrolysis. Liquid chromatography-high resolution mass spectrometry has become the preferred choice for the structural identification of metabolites. This review mainly summarizes research on metabolism software prediction and human hepatocyte model, human liver microsomes model, rat in vivo model, zebrafish model and fungus C. elegans model in metabolite identification based on the structure and classification of synthetic cannabinoids.

Psychoactive Substance and Psychopathological Symptoms among Nigerian Secondary Schools Adolescents

Aim:Abuse of psychoactive substances among secondary school adolescents is a huge problem globally.This study examined the influence of psychoactive substance use on psychopathological symptoms among secondary school adolescents in Ede,Osun State, SouthwesternNigeria. Study Design:Cross-sectional survey design.Place of Study: Redeemer’s University Ede Osun state, Southwestern Nigeria.Methodology: Four hundred and ninety-six (496) secondary school adolescents (mean age 15.7years) were purposively selected from five secondary schools in Ede metropolis. Substance Abuse Inventory (SABI) and Awaritefe Psychological Index (API Form-X)were used for data collection. Descriptive and inferential statisticswere used for data analysis. Four hypotheses were tested using a Regression Analysis, Pearson Product Moment Correlation and Independent Sample t-test and at 0.05% level of significance. Results:A high prevalence of psychopathological symptoms was observed among the participants. Psychoactive substance use was observed to jointly and significantly predict psychopathological symptoms (R²=.106, p = .001). Sex has significant influence on manifested psychopathological symptoms with female adolescents reporting higher mean scores on insomnia, intellect disorder, heat disorder, mood disorder, alimentary track disorder and general psychopathology. Age was observed to be inversely correlated with psychopathological symptoms. Significant difference was found between drug users and non-user on severities of psychopathological symptoms. Conclusions:This research finding indicates a high prevalence of psychopathological symptoms; that the use psychoactivesubstance is significantly linked the psychopathological symptomsand that age of involvement in psychoactive substance is also a significant risk factor for psychopathology among adolescents