ABSTRACT
Objective@#To explore the role of apoliprotein D (APOD) in the proliferation and migration of human dental pulp cells (DPCs) and to provide a basis for the use of APOD to promote pulp regeneration. @*Methods@#APOD expression in human dental pulp cells was inhibited by siRNA. The inhibition effect of APOD was confirmed by qPCR and Western blot. After APOD inhibition, colony formation experiments and CCK8 assays were employed to confirm the proliferation ability of dental pulp cells. Transwell assays were used to verify the cell migration ability after the inhibition of APOD expression.@*Results @# After inhibiting APOD expression, the colony formation rate in the si-apod group was reduced compared with the NC group, and the difference was statistically significant (t=7.624, P=0.002). The CCK8 experiment showed that the OD value in the si-apod group decreased at 3, 5 and 7 d compared with that in the NC group (P < 0.05). Transwell results showed that the number of cell divisions was 57.25 ± 4.03 in the si-apod group and 154.50 ± 8.39 in the NC group, and the difference was statistically significant (t=10.45, P < 0.001).@*Conclusion@# Inhibition of APOD expression in dental pulp cells inhibits their proliferation and migration ability.
ABSTRACT
Pulpal and periapical diseases are the common diseases in human diseases. The traditional treatment method for these diseases is root canal treatment, which is to completely remove and control infection, repair or prevent periapical lesions by root canal mechanical preparation, chemical disinfection and filling. At present, although root canal treatments have a high success rate, there are still a series of problems. Dental pulp regeneration has attracted more and more attention from researchers in promoting the formation of pulp-like tissue in root canals. The ultimate goal of regenerative endodontics is to form a functional endodontic-dentin complex with inner blood vessels and nerves, outer layers of dentin cells arranged along the root canal wall, and new dentin formed by secreting matrix, so as to restore pulp vitality. Stem cells, scaffolds, biosignal molecules, and regenerative microenvironment are key tissue engineering factors that affect pulp regeneration. In this paper, the strategies and applications of pulp regeneration were reviewed around the above factors and clinical procedures.
ABSTRACT
Numerous cases about additional growth of roots or pulp tissue regeneration by using various intracanal medicaments in immature permanent teeth with periapical or pulpal disease have been reported. The underlying mechanism has not been clearly delineated, but it has been widely accepted that undifferentiated mesenchymal cells and stem cells are involved. Moreover, the growth and deposition of osteoid or cementoid tissues have been observed in regenerated pulp and roots. This new and non-invasive treatment has brightened the future of endodontics, and enlarged the vision of regenerative root canal treatment with multi-potent stem cells and various tissue engineering techniques.