ABSTRACT
@#The development of materials science is of great significance to the treatment of dental pulp diseases. Poly lactic acid glycolic acid (PLGA) copolymer is an organic macromolecule compound that is widely used in the preparation of biomedical materials. In recent years, PLGA, as a drug/molecular loaded system and tissue regeneration scaffold, has shown prospects for application in the treatment of dental pulp diseases. This paper will review the application of PLGA in the treatment of dental pulp diseases and provide a basis for its further development and utilization. The results of the literature review show that PLGA is a drug/molecular delivery system that is mainly used in the improvement of pulp capping materials, root canal disinfectant and apexification materials. PLGA-improved pulp capping agents can prolong the action time of the drug and reduce toxicity. The modified root canal disinfectant can realize the sustained release of drug, make the drug penetrate deeper into the subtle structure, and contact more widely with the pathogenic bacteria. The modified apexification materials can provide more convenient administration methods for apexifixment. As a scaffold for tissue engineering, PLGA is mainly used in the study of pulp regeneration. The optimization of PLGA physical properties and action environment can provide a more suitable microenvironment for seed cells to proliferate and differentiate. How to utilize the advantages of PLGA to develop a more suitable material for endodontic application needs further study.
ABSTRACT
@#The aim of vital pulp therapy is to preserve vital, healthy pulp tissue through pulp capping or pulpotomy. Pulp-capping materials are applied to isolate infection, promote odontoblast differentiation and reparative dentine formation during the progress. Calcium silicon-based materials play significant roles in vital pulp therapy with their favorable mechanical properties, bioactivity and biocompatibility. The present paper will review current progress for these novel calcium silicon-based materials used in vital pulp therapy.
ABSTRACT
O hidróxido de cálcio (HCa), o agregado de trióxido mineral (MTA) e o óleo-resina de copaíba (COP) isoladamente apresentam características biológicas do material de capeamento pulpar direto mais apropriado. Com o pressuposto que associados poderiam originar materiais mais apropriados para serem aplicados em capeamento pulpar direto, este estudo objetivou analisar in vitro proliferação, diferenciação e migração de células-tronco de polpa de dente decíduo humano esfoliado (SHEDs) em resposta a substâncias liberadas pelo COP isolado ou associado ao HCa ou ao MTA. Proliferação, diferenciação e migração de SHEDs (Linhagem PDH3) foram analisadas através do ensaio de redução do MTT; da atividade de fosfatase alcalina (ALP), formação de nódulos mineralizados pelo ensaio de Vermelho de Alizarina e expressão dos genes (BGLAP, DSPP, DMP1 e HSP-27) pelo qRT-PCR; e, do ensaio do Scratch, respectivamente. As células foram submetidas à ação de meios condicionados pelos biomateriais, de acordo com os seguintes grupos experimentais: COP isolado (COP); HCa isolado (HCa); HCa associado ao COP (HCa+COP); MTA isolado (MTA) e MTA associado ao COP (MTA+COP). Células crescidas em meio de cultura fresco serviram de controle. Os dados foram comparados utilizando ANOVA complementado pelo teste de Tukey (p 0,05). O grupo HCa apresentou número de células viáveis significativamente menor que o dos demais grupos, inclusive o do grupo HCa+COP (p<0,01) em todos os tempos experimentais...
The calcium hydroxide (CaH), the mineral trioxide aggregate (MTA) and the oil-resin copaiba (COP) by themselves have biological characteristics of the ideal direct pulp capping material. With the assumption that when associated they could originate materials more appropriated for direct pulp capping, this study aimed to analyze the in vitro proliferation, differentiation and migration of stem cells from human exfoliated deciduous teeth (SHEDs) in response to substances leached from COP alone or associated with CaH or MTA. Proliferation, differentiation and migration of SHEDs (PDH3 lineage) were analyzed through the MTT reduction assay; alkaline phosphatase (ALP) activity, mineralized nodules formation using the Alizarin Red assay and gene expression (BGLAP, DSPP, DMP1 e HSP-27) using qRT-PCR; and the Scratch assay, respectively. The cells were submitted to the culture medium conditioned by the biomaterials according to the following experimental groups: COP alone (COP); CaH alone (CaH); CaH associated to COP (CaH+COP); MTA alone (MTA) and MTA associated to COP (MTA+COP). Cells grown in fresh culture medium served as control. Data were compared by ANOVA complemented by the Tukey´s test (p 0.05). The CaH group presented number of viable cells significantly smaller (p<0.01) than those of all other experimental groups, including the CaH+COP, during whole experimental time. The ALP activity in 14 days was similar in all experimental groups. In 21 days, the COP group presented the amount of mineralization higher (p<0.01) than those of all other groups. The gene DMP1 was not expressed by the cells in all experimental groups. The COP group presented the smallest expression (p<0.01) of BGLAP, DSPP and HSP-27 genes. The SHEDs of the MTA+COP group presented superexpression of the BGLAP, DSPP and HSP-27 genes, and in the CaH+COP group the cells superexpressed the BGLAP and HSP-27 genes...
Subject(s)
Biocompatible Materials , Stem Cells/metabolism , Dental Pulp CappingABSTRACT
Objetivo: Avaliar o pH e liberação de íons cálcio dos materiais forradores a base de hidróxido de cálcio não fotopolimerizável (Hydro C), um fotopolimerizável (Biocal) e MTA. Os materiais foram manipulados e inseridos em tubos de 1 cm de comprimento e 1 mm de diâmetro. Os tubos foram preenchidos e imediatamente imersos em frascos contendo 10 mL de água deionizada. Os tubos foram removidos dos frascos depois de 10 minutos, 24, 48 horas, 7, 15 e 30 dias, e a liberação de íons cálcio e hidroxila foi mensurada com um pHmetro e espectrofotômetro de absorção atômica, respectivamente. Os dados foram comparados pela análise de variância a dois critérios, e as comparações individuais pelo teste de Tukey-Kramer, com nível de significância de 5%. Com relação a liberação de íons calcio, no periodo de 10 minutos ocorreram diferenças significantes (P<0,05) nas comparações do MTA com os outros dois materiais. Nos períodos de 24 e 48 horas os três materiais se diferenciaram estatisticamente (P<0,05) entre si. Na análise de 7 e 15 dias ocorreram diferenças significantes (P<0,05) nas comparações entre: MTA e Biocal, e no confronto entre Hydro C e Biocal. Na avaliação de 30 dias as diferenças significantes (P<0,05) ocorreram nas comparações do MTA com os demais materiais. Com relação ao pH, no período de 10 minutos e 24 horas não ocorreram diferenças significantes (P>0,05). Nos períodos de 48 horas ocorreu diferença estatística (P<0,05) nas comparações do BioCal com os outros dois materiais. Na análise de 7 dias ocorreu diferença significante (P<0,05) na comparação entre MTA e Biocal. Na avaliação de 15 dias ocorreram diferenças significantes (P<0,05) na comparação do Hidro C com os demais materiais. Na análise de 30 dias ocorreram diferenças significantes (P>0,05) nas comparações. Conclui-se que todos os materiais foram capazes de liberar íons cálcio e hidroxila.
Objective: Evaluate the pH and calcium ion release from the pulp-capping materials based on calcium hydroxide does not light-cured (Hydro), a light-cured (Biocal) and MTA. The materials were manipulated and inserted into tubes of 1 cm in length and 1 mm in diameter. The tubes were completed and immediately immersed in vials containing 10 mL of deionized water. The tubes were removed from bottles after 10 minutes, 24, 48 hours, 7, 15 and 30 days, and calcium and hydroxyl ions release was measured with an atomic absorption spectrophotometer and pHmetro, respectively. The data were compared by analysis of variance on two criteria, and individual comparisons by Tukey-Kramer test with significance of 5%. Regarding the calcium ion release, in period of 10 minutes there were significant differences (P<0 .05) MTA comparisons with the other two materials. During periods of 24 and 48 hours the three materials have differentiated statistically (P<0 .05) among themselves. Analysis of 7 and 15 days there were significant differences (P<0 .05) in the comparisons between: MTA and the confrontation between Biocal and Hydro C and Biocal. 30 days evaluation of significant differences (P<0 .05) occurred in MTA comparisons with other materials. With regards to pH, within 10 minutes and 12:0 am not significant differences occurred (P>0 .05). During periods of 48 hours there was statistical difference (P0 .05) in BioCal's comparisons with the other two materials. In During periods of 48 hours there was statistical difference (P<0 .05) in BioCal's comparisons with the other two materials. Analysis of 7 days there was significant difference (P<0 .05) in the comparison between MTA and Biocal. In 15 days trial significant differences occurred (P<0 .05) in comparison with the other's Hydro C materials. In the analysis of significant differences occurred 30 days (P<0 .05) in the comparisons. It is concluded that all materials were able to release calcium ions and hydroxyl.