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ABSTRACT: Aster (Aster ericoides L.) is a flower crop of North American origin, which belongs to the family Asteraceae. This plant presents capitulum-type inflorescences, widely used as cut flowers. In Brazil, it has been grown only recently, but still has a high expansion potential. In floriculture, pulsing and conditioning solutions are often used to extend the vase life of cut flowers. The present study aimed to evaluate the influence of silver thiosulphate (STS) pulsing and sucrose solutions on the postharvest life of A. ericoides stems. The experiment was carried out under a complete randomized design with treatments arranged in a 2x5 factorial scheme. Treatments consisted of flower stems treated with STS pulsing for 30 minutes, combined with four sucrose concentrations (5, 10, 15, and 20%) for six hours, plus a control (without treatment). Each plot contained four replicates of 10 stems, totalizing 400 stems. The following variables were daily: vase life, fresh mass, and relative water content (RWC). For pulsing data, means were compared by the Tukey's test (p≥5%). Yet for sucrose concentrations, means were submitted to a polynomial regression to verify the behavior of variables as a function of concentrations increases. Likewise, each treatment was evaluated for fresh mass and RWC variations over time by a polynomial regression. Results indicated no effect of STS pulsing (2mM) on the vase-life length of aster stems. In contrast, the sucrose solution at 10% extended the vase life of stems by two days, decreased fresh mass loss, and helped maintain water balance in stems.
RESUMO: O áster (Aster ericoides L.) é originário da América do Norte, pertence à família Asteraceae, possui inflorescências do tipo capítulo e é muito usada como flor de corte. No Brasil, sua produção é recente e apresenta grande potencial de expansão. O uso de soluções de "pulsing" e de acondicionamento, em flores de corte, é bastante empregado na floricultura para prolongar o tempo de prateleira. O objetivo do presente estudo foi avaliar a influência do "pulsing" de STS (tiossulfato de prata) e do condicionamento em soluções de sacarose na vida pós-colheita de hastes de A. ericoides. O delineamento experimental foi inteiramente casualizado e os tratamentos foram arranjados em esquema fatorial 2x5, ou seja, com e sem aplicação de "pulsing" com tiossulfato de prata (STS) 2mM por 30 minutos, combinados com quatro concentrações de sacarose: 5, 10, 15 e 20% por 6 horas e tratamento controle; foram 4 repetições e 10 hastes por parcela, perfazendo um total de 400 hastes. Avaliou-se diariamente a vida de vaso, acúmulo de massa fresca e conteúdo relativo de água (CRA) das hastes tratadas. Os dados de "pulsing" (STS) as médias foram comparadas pelo Teste de Tukey a 5% de probabilidade. As médias de sacarose foram submetidas à análise de regressão polinomial a fim de verificar o comportamento das variáveis em função do aumento da concentração de sacarose e para massa fresca e CRA foi realizada regressão polinomial para cada tratamento a fim de verificar o comportamento do tratamento ao longo do tempo. Concluiu-se que tratamento de "pulsing" com tiossulfato de prata (STS) a 2mM por 30 minutos não contribuiu para o aumento da vida de vaso das hastes de áster. O tratamento de condicionamento com 10% de sacarose prolongou a vida de vaso em dois dias, diminuiu a perda de massa fresca e favoreceu o balanço hídrico de hastes de áster.
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Objective To study the effect of the continuous pulsing water ball in preventing infection in the multi-level posterior lumbar interbody fusion(PLIF).Methods From June 2016 to December 2016,thirty patients who were treated with multi-level posterior lumbar interbody fusion in our hospital were randomly divided into two groups.The 15 patients in the observation group were given continuous pulsing water ball,and the other 15 patients in the control group were given conventional dumping.The items including the time of intraoperative washing,the C-reactive protein(CRP),the time of suture clearing,postoperative fever cases and postoperative incision healing were oberved and compared between the two groups.Results The time of intraoperative washing was (2.13±0.15)min in the observation group and (5.59±1.24)min in the conrtrol group,and the difference was statistically significant (P<0.05).The postoperative CRP of 1st,3rd, and 5th day in the observation group was declining,whileas the control group was increasing.The time of suture clearing in the oberation group was 11.57 d, which was significantly less than 14.29 d the control group.The temperature of 3rd and 5th day after opreation in the obervation group was (37.1±2.26)℃ and (37.0±0.12)℃ respectively, which were significantly lower than (38.2±3.34)℃ and (37.5±0.25)℃ respectively the control group,and the difference was statistically significant(P<0.05).Conclusion Compared to the the conventional dumping,the continuous pulsing water ball has the some advantages in the multi-level posterior lumbar interbody fusion (PLIF),such as decreasing the washing time and reducing the local inflammatory reponse.
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Objective To evaluate the value of reducing radiation dose with decreased size of ECG-pulsing windows and influence on image quality in dual-source CT coronary angiography. Methods 120 patients with stable heart rate(HR) were divided into four groups according to HR and the rang of ECG-pulsing windows in dual-source CT coronary angiography: HR ＜ 70 bpm and 61% ～ 77% R-R interval of ECG-pulsing windows, HR ＜ 70 bpm and 25% ～ 80% R-R interval of ECG-pulsing windows,HR ＞ 80 bpm and 31% ～47% R-R interval of ECG-pulsing windows, and HR ＞ 80 bpm and 25% ～ 80%R-R interval of ECG-pulsing windows was employed, respectively. The radiation dose parameters were recorded and image quality scores were performed. The image quality and radiation dose between two slow HR groups and between two fast HR groups were compared respectively. Result The effective doses were (7.06 ± 2. 13 ), ( 11.34 ± 3.65 ), ( 6. 67 ± 1.97 ) and ( 9. 92 ± 3. 15 ) mSy for four groups, respectively.The effective dose was decreased by 37.74% for slow HR and by 32. 76% for fast HR using narrow ECG-pulsing windows. There was no difference on image quality between two slow HR groups and two fast HR grouvs. Concluslons The proper application of narrow ECG-pulsing windows can reduce radiation exposure significantly to stable slow or fast HR patients in dual-source CT coronary angiography withont sacrificing the image quality.
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A orquídea Oncidium varicosum é bastante utilizada como flor de vaso e atualmente vem se destacando como flor de corte. Devido sua baixa resistência pós-colheita, o objetivo deste trabalho foi avaliar a longevidade de Oncidium varicosum após o acondicionamento em soluções conservantes com diferentes concentrações de sacarose. As hastes de Oncidium varicosum, produzidas em Holambra (SP), foram transportadas para Londrina (PR) em caminhão climatizado a 5-7°c. Foram avaliadas a utilização de pré-tratamento com "pulsing" (15 de sacarose por 24 horas) seguido de acondicionamento em solução de manutenção (1,2,3 e 4 de sacarose) e o acondicionamento em solução de manutenção sem pré-tratamento. As hastes florais foram distribuídas em delineamento inteiramente casualizado, com dez tratamentos e cinco repetições. As avaliações das flores foram diárias, sob critério de notas definido para comparar e verificar a conservação dos aspectos visuais. Nenhum dos tratamentos demonstrou superioridade à testemunha na manutenção da longevidade das flores, que foi de três a quatro dias.
Oncidium varicosum from the Orchidaceae family has been widely used as pot flower, and, most recently,as cut flower. The objective of this work was to evaluate the longevity of the Oncidium varicosum, dueto its low post-harvest resistance, after being conditioned in preservation solutions with differentconcentrations of sucrose. Oncidium varicosum stems grown in Holambra (SP) were transported toLondrina (PR) in climatized trucks at 5-7oC. Pre-treatment with pulsing (15% sucrose for 24 hours)followed by conditioning in maintenance solution (1, 2, 3, and 4% of sucrose) as well as conditioning inmaintenance solution without pre-treatment were evaluated. Flower stems were distributed in an entirelycasualized design, with ten treatments and five replications. Flowers were evaluated daily, using a scoresystem developed to compare and verify the conservation of visual aspects. No treatment was superiorto the control in maintaining the longevity of the flowers
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Flowers/growth & development , SucroseABSTRACT
BACKGROUND: Tumor cell lysate has been considered as a preferential antigen source for the therapeutic dendritic cell pulsing. Our experiences with in vivo study with animal tumor model indicate the tumor cell lysate dependent differential effect of DC therapy. Our previous data show that MC38 lysate pulsed-DC induced stronger ag-specific immunity than CT26 lysate pulsed-DC in vitro. In this study we tried to reveal the mechanism for differential induction of ag-specific immunity of different colon cancer cell lysate pulsed-DCs. METHODS: MC38 and CT26 cell lines were prepared as lysate by freezing-thawing procedure. Tumor cell antigenicity was confirmed by detecting the surface expression of MHC I/II & B7.1/2 molecules. IL-10, IL-12 and TGF-beta in the tumor cell lysate were detected by ELISA and the presence of heat shock proteins were analysed by western blotting. RESULTS: The secretion of IL-10, a immune-inhibitory cytokine was about 470% higher in CT26 lysate than in MC38. Hsp 70 was detected only in the MC38 lysate but not in the CT26. On the other hand, Hsp 60 and 90 expression were not different in two colon cancer cell lysates. CONCLUSION: In two different colon caner cell lysate, immune inhibitory IL-10 (higher in CT26) and Hsp70 (MC38 superiority) were differentially expressed. These data indicate that higher ag- specific immunity induction by MC38 lysate pulsed-DC may due to the expression of hsp70 and lower secretion of IL-10, a immune-inhibitory cytokine than CT26 lysate. The significance of other cytokine and the surface marker expression will be discussed.
Subject(s)
Animals , Blotting, Western , Cell Line , Colon , Colonic Neoplasms , Dendritic Cells , Enzyme-Linked Immunosorbent Assay , Hand , Heat-Shock Proteins , Interleukin-10 , Interleukin-12 , Transforming Growth Factor betaABSTRACT
Objective To study the effect of pulsing magnetic field (PMF) on the osteogenesis of the distracted region in cosmetic limb lengthening, and to search for an effective therapeutic method for promoting the recovery of the cosmetic patient. Methods Thirty adult short stature volunteers for cosmetic heightening were randomly divided into PMF group and control group, 15 in each group. Magnetic stimulation was given to the PMF group on the day after operation. X-ray examination was taken on fixed interval, and lengthening index was used for bone healing evaluation. Results The height increase ranged from 5～9 cm, mean (6.6?1.2) cm, and the mean lengthening rate was 19.2 % : 5～8 cm (6.2?1.1) cm in the PMF group and 5.2～9 cm (6.8?1.6)cm in the control group ( P
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This paper analyzes the disqualification factors influencing B-D test results by using B-D test monitoring. Effective methods were taken to remove them and the problems were found in time for overhauling the sterilizer. The forevacuum steam sterilizer should be kept in good state and regulated strictly so that it can work in program with qualified sterilizing quality,thus ensuing a safe and reliable clinical application.