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1.
Journal of Prevention and Treatment for Stomatological Diseases ; (12): 901-906, 2023.
Article in Chinese | WPRIM | ID: wpr-988597

ABSTRACT

@#Periodontitis is associated with abnormal purine metabolism, which is manifested by increased uric acid in host blood and increased expression of the purine-degrading enzyme, xanthine oxidoreductase (XOR), in periodontal tissues. Both XOR and uric acid are pro-oxidative and pro-inflammatory mediators under pathological conditions. Animal studies have found that injection of uric acid promotes the progression of periodontitis and that febuxostat (an XOR inhibitor) improves tissue destruction in periodontitis. Therefore, blocking the source of uric acid may be a therapeutic strategy to control the progression of periodontitis. In this article, the rationality of XOR inhibitors as potential therapeutic drugs for periodontitis is reviewed. The literature review results suggest that XOR inhibitors show antioxidative, anti-inflammatory, and anti-osteoclastic effects, and XOR inhibitors show clinical efficacy in the treatment of infectious, inflammatory and osteolytic diseases. Although there is no direct evidence to support the finding that XOR inhibitors can ameliorate periodontal microecological dysbiosis, these drugs can modulate intestinal microflora dysbiosis, and there is indirect evidence to support a beneficial effect of XOR inhibitors on periodontal microecological dysbiosis. In conclusion, XOR inhibitors may be used as immunomodulators for the adjuvant treatment of periodontitis by inhibiting inflammation, oxidative stress and anti-osteoclast effects.

2.
Chinese Pharmacological Bulletin ; (12): 201-206, 2023.
Article in Chinese | WPRIM | ID: wpr-1013843

ABSTRACT

Hyperuricemia is a chronic metabolic disease caused by purine metabolism disorder or uric acid excretion disorder. The experimental animal model of hyperuricemia is the basis for studying the pathological mechanism and drug treatment of hyperuricemia. This paper reviews the experimental animal models of hyperuricemia commonly used in drug research, and introduces the modeling principle, preparation methods, species selection and related detection techniques of the models, so as to provide reference for the application of such models in research.

3.
China Journal of Chinese Materia Medica ; (24): 2193-2202, 2023.
Article in Chinese | WPRIM | ID: wpr-981350

ABSTRACT

This study aims to explore the effect of tryptanthrin on potential metabolic biomarkers in the serum of mice with ulcerative colitis(UC) induced by dextran sulfate sodium(DSS) based on liquid chromatography-mass spectrometry(LC-MS) and predict the related metabolic pathways. C57BL/6 mice were randomly assigned into a tryptanthrin group, a sulfasalazine group, a control group, and a model group. The mouse model of UC was established by free drinking of 3% DSS solution for 11 days, and corresponding drugs were adminsitrated at the same time. The signs of mice were observed and the disease activity index(DAI) score was recorded from the first day. Colon tissue samples were collected after the experiment and observed by hematoxylin-eosin(HE) staining. The levels of interleukin-4(IL-4), interleukin-10(IL-10), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-8(IL-8) in the serum were measured by enzyme linked immunosorbent assay(ELISA). The serum samples were collected from 6 mice in each group for widely targeted metabolomics. The metabolic pathways were enriched by MetaboAnalyst 5.0. The results showed that compared with the model group, tryptanthrin treatment decreased the DAI score(P<0.05), alleviated the injury of the colon tissue and the infiltration of inflammatory cells, lowered the levels of proinflammatory cytokines, and elevated the levels of anti-inflammatory cytokines in the serum. The metabolomic analysis revealed 28 differential metabolites which were involved in 3 metabolic pathways including purine metabolism, arachidonic acid metabolism, and tryptophan metabolism. Tryptanthrin may restore the metabolism of the mice with UC induced by DSS to the normal level by regulating the purine metabolism, arachidonic acid metabolism, and tryptophan metabolism. This study employed metabolomics to analyze the mechanism of tryptanthrin in the treatment of UC, providing an experimental basis for the utilization and development of tryptanthrin.


Subject(s)
Mice , Animals , Colitis, Ulcerative/drug therapy , Tryptophan , Arachidonic Acid/metabolism , Mice, Inbred C57BL , Colon , Cytokines/metabolism , Tumor Necrosis Factor-alpha/metabolism , Interleukin-6/metabolism , Metabolomics , Purines/therapeutic use , Dextran Sulfate/metabolism , Disease Models, Animal , Colitis/chemically induced
4.
Chinese Journal of Urology ; (12): 156-160, 2022.
Article in Chinese | WPRIM | ID: wpr-933184

ABSTRACT

Some kidney stones are caused by single gene mutations, and monogenic kidney stone diseases associated with purine metabolic disorder mainly including adenine phosphoribosyltransferase(APRT) deficiency, hypoxanthine-guanine phosphoribosyltransferase(HPRT)deficiency, hereditary xanthinuria(HX), and some diseases caused by gene mutations such as PRS1, SLC22A12, SLC2A9 and ABCG2. Such diseases can lead to abnormal metabolism of purine and uric acid, and then form 2, 8-dihydroxyadenine stones, uric acid stones or xanthine stones. This kind of diseases are rare, the genotype and phenotype of different types of monogenic diseases related to purine metabolism have their own characteristics and are not widely recognized. At present, the main treatment is medical therapy. Gene sequencing will make the diagnosis and find more disease-related genes or mutations. Gene editing, such as CRISPR/Cas9 technology, makes it possible to cure monogenic kidney stone diseases associated with purine metabolism disorder in the future.

5.
Acta Pharmaceutica Sinica ; (12): 2464-2471, 2021.
Article in Chinese | WPRIM | ID: wpr-886942

ABSTRACT

Depression was a complex and difficult to regulate disease, which was closely related to purinergic system and purine metabolism disorder. Although there had been studies to improve depression by regulating purinergic system, the mechanism of action was complex and needed to be sorted out. Recently, a large number of studies had found that the addition of exogenous purine metabolites adenosine, inosine and guanosine had a significant antidepressant effect, indicating that regulating the level of purine substances in purine metabolism could also improve depression, which was of great significance to the further study of the pathogenesis and treatment of depression. In view of this, this study reviewed the relationship between purinergic system or purine metabolism and depression, in order to provide a reference for the further study of the pathogenesis of depression.

6.
Biosci. j. (Online) ; 36(4): 1292-1299, 01-06-2020. tab
Article in English | LILACS | ID: biblio-1147243

ABSTRACT

This study investigated the effectiveness of the urine sample collection method in predicting the volume urinary and synthesis of microbial nitrogen. Eight fistulated steers were used with accessible rumens and kept in individual stalls. Their diets consisted of corn silage; corn silage + concentrate; corn silage + concentrate with addition of lipids in the form of soybean oil; and corn silage + concentrate with addition of lipids in the form of soybean grains. Estimates of microbial protein synthesis were obtained based on the urinary excretion of purine derivatives. There was no effect of diets on daily creatinine excretion (P>0.05). There were differences (P<0.05) between the urinary volume and microbial synthesis values determined by the total urine collection and those estimated from the urine spot samples and equations proposed by different authors. The estimation of microbial synthesis based on the urine excretion of purine derivatives should be performed from the total collection of the urine for a period of 24 hours.


Este estudo investigou a eficácia do método de coleta de amostras de urina sobre a predição do volume urinário e síntese de nitrogênio microbiano. Oito novilhos fistulados foram utilizados com rúmen acessível e mantidos em baias individuais. Suas dietas consistiram de silagem de milho; silagem de milho + concentrado; silagem de milho + concentrado com adição de lipídios na forma de óleo de soja; e silagem de milho + concentrado com adição de lipídios na forma de grãos de soja. Estimativas de síntese de proteína microbiana foram obtidas com base na excreção urinária de derivados de purina. Não houve efeito de dietas na excreção diária de creatinina (P> 0,05). Houve diferenças (P <0,05) entre o volume urinário e os valores de síntese microbiana determinados pela coleta total de urina e os estimados a partir das amostras de manchas de urina e equações propostas por diferentes autores. A estimativa da síntese microbiana baseada na excreção de urina dos derivados de purina deve ser realizada a partir da coleta total da urina por um período de 24 horas.


Subject(s)
Cattle , Animal Feed , Urine , Creatinine
7.
Journal of Rural Medicine ; : 225-229, 2020.
Article in English | WPRIM | ID: wpr-829820

ABSTRACT

Objective: Immunosuppressive therapy for interstitial lung disease (ILD) is often necessary, but the standard regimen for antisynthetase-associated ILD has not been established.Patient: An 80-year-old man was hospitalized for severely progressive dyspnea. Bilateral interstitial shadows occurred 1 month before the event. Serological findings showed that he had antisynthetase-associated ILD, as identified by strong positivity for anti-aminoacyl-transfer RNA synthetase (ARS) antibody, despite no evidence of myositis. He was treated transiently with noninvasive positive pressure ventilation and steroid-pulse therapy followed by 60 mg/day of oral prednisolone. However, his diabetes mellitus was aggravated by corticosteroid therapy; thus, a combination of low-dose steroid and mizoribine (MZB), which has a low risk of aggravating glucose intolerance, was used.Results: The patient’s clinical symptoms and daily life activities have been well persevered as an outpatient and well maintained with 200 mg of MZB and 10 mg of prednisolone for several months without obvious clinical recurrence and without any remarkable steroid- and MZB-related side effects.Conclusion: The use of MZB appeared to suppress the pathophysiology of anti-ARS antibody-associated ILD.

8.
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 135-141, 2019.
Article in Chinese | WPRIM | ID: wpr-802012

ABSTRACT

Objective: To explore the interrelation of "composition-target-disease" of Kaixinsan on treatment of Alzheimer's disease. Method: Through the integrated pharmacological platform of Chinese medicine V1.0,the active ingredients and potential targets of four Chinese herbs in Kaixinsan were collected,disease targets of Alzheimer's disease were searched,and enriched by the gene ontology database and the Kyoto encyclopedia of genes and genomes at hubs. Result: Among the 250 compounds of Kaixinsan,2 877 targets were associated with Alzheimer's disease.The key targets,such as mitochondrial trifunctional enzyme subunit alpha(HADHA),hydroxyacyl coenzyme A dehydrogenase(HADH),sterol-4-alpha-carboxylate 3-dehydrogenase(NSDHL) and others,played their pharmacological effects mainly through regulating purine and nucleotide metabolism,Huntington's disease,Alzheimer's disease,neurodegenerative diseases,oxidative phosphorylation,and endocrine and metabolic diseases in molecular reactions,such as cytoplasm,mitochondria,adenosine triphosphate binding,and mitochondrial matrix. Conclusion: The platform can predict the key targets and related pathways of Kaixinsan for treatment of Alzheimer's disease,which lays the foundation for further revealing material basis and mechanism of this formula,and plays an important role in digging and developing this classic and famous formula.

9.
Acta Anatomica Sinica ; (6): 280-284, 2019.
Article in Chinese | WPRIM | ID: wpr-844652

ABSTRACT

Objective To investigate the cflecl of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4., spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OFCs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method. The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situ hybridization were observed in L4-J spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased(P<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord significantly increased(PcO. 05) compared with the CCI group, the reflex threshold of mcchaniral withdrawl of rats in the OF.Cs group was significantly increased (/'cO. 05). The percentage and average absorbance of P2X7 receptor positive cells in L«., spinal cord significantly decreased compared with the OECs group(P<0. 05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher(/,<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord were lower(H<0. 05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain belter and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.

10.
Chinese journal of integrative medicine ; (12): 285-291, 2019.
Article in English | WPRIM | ID: wpr-776619

ABSTRACT

OBJECTIVE@#To quantify the purine concentrations of the acupoints along the pericardium and nonpericardium meridians under healthy and myocardial ischemia conditions to investigate the relationship between acupoint purine change and body functional status in rats.@*METHODS@#A total of 70 rats underwent an operation for myocardial ischemia, while 40 of them survived. They were randomly assigned to the following 5 subgroups: Neiguan (PC 6), Quze (PC 3), Tianquan (PC 2), Quchi (LI 11), and Jianyu (LI 15). Simultaneously, another 40 healthy rats were also randomized into the same 5 subgroups as the control group. The tissue fluids at the acupoints were collected by microdialysis for 30 min. Subsequently, the concentration of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and adenosine (ADO) were quantified using the high-performance liquid chromatography method.@*RESULTS@#Compared with the healthy group, the ADO at PC 6 (P=0.012), PC 3 (P=0.038), PC 2 (P=0.024), and LI 15 (P=0.042) obviously increased in the model group, while no significant difference was observed at LI 11 (P=0.201). However, ATP, ADP, and AMP manifested no significant changes in these areas, except for ATP at LI 15 (P=0.036).@*CONCLUSIONS@#Myocardial ischemia could induce an increase in ADO at acupoints of the upper arm and shoulder area, suggesting that the body functional status could affect the responsiveness of acupoints. The status of these acupoints could be pathogenically activated by disease, and distribution following some specific courses.

11.
Chinese Pharmacological Bulletin ; (12): 450-454, 2017.
Article in Chinese | WPRIM | ID: wpr-511280

ABSTRACT

The function of the nervous system is largely determined by the communication between neurons and glial cells.The interaction of complex signaling pathways between neurons and glial cells involves in a variety of signaling molecules.Purines and the purinergic receptors play a crucial role in the process of the interaction.For a long time, the study on the central nervous system is mainly focused on neurons, while glial cells are mainly used as support cells or in response to brain injury to provide a guiding role.The latest research shows that glial cells play an important role in the occurrence and development of nervous system diseases.We will summarize the function of purinergic receptors in the interaction of neurons and glial cells and in neurological diseases.

12.
Chinese Pharmacological Bulletin ; (12): 883-886, 2017.
Article in Chinese | WPRIM | ID: wpr-618936

ABSTRACT

Aim To establish a novel acute hyperuricemia mouse model and apply it to evaluate the hyporucicemia effects of Ulodesine, a purine nucleoside phosphorylase(PNP) inhibitor.Methods The mice were intraperitoneal injected inosine and subcutaneous injected Oteracil potassium to induce accumulation of uric acid, and the animal blood was collected from eyeball or vena angularis in different time points.The levels of serum uric acid were measured and determined to test whether the acute hyperuricemia mouse model were successful or not.In order to verify the hyperuricemia seen in the model was associated with the accumulation of inosine, which was converted to uric acid by action of PNP,hyporucicemia effects of Ulodesine, a PNP inhibitor, was assessed in an enzyme assay and confirmed by using the newly established model.Result Accumulation of uric acid in the blood of mouse models was observed by combined injections of intraperitoneal 200 mg·kg-1 inosine and subcutaneous 200 mg·kg-1 Oteracil potassium respectively after 1.5 h.The enzyme assay indicated that Ulodesine was a potently PNP inhibitor with IC50 of 2.293 nmol·L-1.IV injection of Ulodesine eliminated uric acid accumulations in blood of the mouse model, which was expected as the in vivo action of Ulodesine.Conclusions A novel acute hyperuricemia mouse model is established.This is a relatively easy and more effective protocol to generate the hyperuricemia in mice, which will be a useful platform to assess the anti-hyperuricemia activity of PNP-target drugs in vivo.

13.
Braz. j. pharm. sci ; 52(4): 787-794, Oct.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-951889

ABSTRACT

ABSTRACT Heroin is known to enhance catabolism and inhibit anabolism of purine nucleotides, leading to purine nucleotide deficiencies in rat brains. Here, we determined the effect of exogenous purine nucleotide administration on purine nucleotide metabolism in the brains of heroin-dependent rats. Heroin was administrated in increasing doses for 9 consecutive days to induce addiction, and the biochemical changes associated with heroin and purine nucleotide administration were compared among the treated groups. HPLC was performed to detect the absolute concentrations of purine nucleotides in the rat brain cortices. The enzymatic activities of adenosine deaminase (ADA) and xanthine oxidase (XO) in the treated rat cortices were analyzed, and qRT-PCR was performed to determine the relative expression of ADA, XO, adenine phosphoribosyl transferase (APRT), hypoxanthine-guaninephosphoribosyl transferase (HGPRT), and adenosine kinase (AK). Heroin increased the enzymatic activity of ADA and XO, and up-regulated the transcription of ADA and XO. Alternatively, heroin decreased the transcription of AK, APRT, and HGPRT in the rat cortices. Furthermore, purine nucleotide administration alleviated the effect of heroin on purine nucleotide content, activity of essential purine nucleotide metabolic enzymes, and transcript levels of these genes. Our findings therefore represent a novel, putative approach to the treatment of heroin addiction.


Subject(s)
Animals , Male , Rats , Purine Nucleosides/analysis , Purine Nucleotides/adverse effects , Heroin/adverse effects , Xanthine Oxidase/analysis , Adenosine Deaminase/analysis , Heroin Dependence/classification
14.
Chinese Acupuncture & Moxibustion ; (12): 669-672, 2016.
Article in Chinese | WPRIM | ID: wpr-352633

ABSTRACT

Coronary heart disease (CHD) is believed to be one of the major diseases with myocardial ischemia (MI) as basic pathological change. The clinical trials and experimental researches in the last 15 years were analyzed in this paper, and it was found that purinergic signaling could transduce the selective expression of molecular adenosine receptors A, A, A, Ain cardiovascular system, which had the potential to be a novel target for treating cardiovascular disease. Using purinergic receptors to explore the actions of acupuncture for myocardial ischemia provides new perspectives to elucidate the mechanism of acupuncture for MI.

15.
Chinese Pharmacological Bulletin ; (12): 1044-1048, 2016.
Article in Chinese | WPRIM | ID: wpr-495697

ABSTRACT

MTH1 enzyme belongs to the family of Nudix hydro-lases, which can prevent the oxidative damage of nucleic acid by cleaning up oxidized purine nucleotides .Close relationships were found between the activity of MTH 1 and neurodegenerative dis-eases, anti-aging et al.In recent years, MTH1 has been regar-ded as a new promising target for cancer treatment , because it plays an essential role in tumor growth , survival , invasion and metastasis.The transcription and translation , structure, catalyt-ic mechanism, detection and application of MTH1 were de-scribed, as well as various MTH1 inhibitors.

16.
Chinese Pharmaceutical Journal ; (24): 1726-1729, 2016.
Article in Chinese | WPRIM | ID: wpr-858932

ABSTRACT

Cardiac hypo-function caused by myocardial ischemia is considered as the first killer of human health. The related metabolites in body can be changed due to the lack of blood and oxygen. These metabolites generated by pathological and physiology changes or exogenous intervention are extreme complicated, and the existing test methods can't satisfy the prospected purpose due to the lower sensitivity, more narrow liner range and so on. Therefore, the concept of metabonomics, a systemic analysis method, was put forward. Metabolites can be quantified in temporal and spatial dimension by metabonomics featured in a high throughput, higher detective sensitivity and wider liner range manner. So it has gradually been applied to many areas including myocardial ischemia. In this paper, based on the comprehensive research literature in domestic and overseas in recent years, the application of metabonomics in pharmacological research of myocardial ischemia was summarized. Five kinds of metabolic pathways are related with myocardial ischemia including sugar metabolism, energy metabolism, amino acid metabolism, purine metabolism and soluble epoxide hydrolase table oxidase P450 metabolism. Some drugs can be used to myocardial ischemia via these pathways. These representative drugs and their mechanisms which are analyzed by metabonomics were concluded in this paper.

17.
Ciênc. rural ; 45(8): 1445-1450, 08/2015. tab, graf
Article in Portuguese | LILACS | ID: lil-753066

ABSTRACT

The objective of this research was to verify the potential rhizogenic stem cuttings and root of raspberry (Rubus idaeus L.). Cuttings of 'Batum' cultivar was stored cold (4°C) for 10, 20 and 30 days, and a control without storage. Subsequently, the cuttings were treated with indolebutyric acid (IBA) at 0, 1000, 2000, 3000 and 4000mg L-1 and root cuttings with benzylaminopurine (BAP) at 0, 300, 600, 900, 1200 and 1500mg L-1 by 10 seconds. The cuttings were buried in 2/3 of its length in the vertical position, and root was completely buried in the horizontal position at three inches deep. It was used vermiculite medium as substrate, and these experiments were conducted in a greenhouse with 50% shading. Evaluations were made ​​75 days after planting the cuttings. It was concluded that the use of cuttings is not feasible and that the root cuttings need not be stimulated by cold storage or treatment with BAP.


O objetivo deste trabalho foi verificar o potencial rizogênico de estacas caulinares e radiculares da framboeseira (Rubus idaeus L.). Estacas da cultivar 'Batum' foram armazenadas a frio (4ºC) por 10, 20 e 30 dias, além do controle sem armazenamento. Posteriormente, as estacas caulinares foram tratadas com ácido indolilbutírico (AIB) a 0, 1000, 2000, 3000 e 4000mg L-1 e as estacas radiculares com benzilaminopurina (BAP) a 0, 300, 600, 900, 1200 e 1500mg L-1 por 10s. As estacas caulinares foram enterradas em 2/3 de seu comprimento, na posição vertical e as radiculares foram totalmente enterradas na posição horizontal, a três centímetros de profundidade. Utilizou-se vermiculita de grânulos médios como substrato, e estes experimentos foram conduzidos sob telado com sombreamento de 50%. As avaliações foram feitas após 75 dias do plantio das estacas. Concluiu-se que o uso de estacas caulinares não é viável e que as estacas radiculares não necessitam ser estimuladas pelo armazenamento a frio ou do tratamento com BAP.

18.
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care ; (6): 154-156, 2015.
Article in Chinese | WPRIM | ID: wpr-460303

ABSTRACT

Objective To investigate the effect of Shenmai injection on purine content in rat cerebral cortex in order to provide a theoretical basis concerning its brain protective mechanism. Methods Sixteen Sprague-Dawley (SD) rats were randomly divided into two groups:normal saline control group and Shenmai injection group, with 8 rats in each group. Shenmai injection 15 mL/kg was injected intraperitoneally into the rats in Shenmai injection group, while in the normal saline group, an equal volume of normal saline was intraperitoneally injected. After the injection for 24 hours, the rats were sacrificed, and the cerebral cortex was removed on ice, homogenized and its supernatant was extracted;then high performance liquid chromatography (HPLC) was used to detect adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine and inosine contents in the supernatant of cerebral cortex. Results Compared with normal saline control group, ATP, ADP, AMP, adenosine and creatinine content in the cerebral cortex of Shenmai injection group were significantly higher, the differences being statistically significant [ATP (ng/L): 31.62±5.12 vs. 20.25±4.53, ADP (ng/L): 37.04±6.72 vs. 25.12±7.35, AMP (ng/L): 87.82±20.37 vs. 33.23±10.34, adenosine (ng/L): 2.82±0.15 vs. 1.12±0.61, creatinine (ng/L): 11.72±1.05 vs. 6.05±2.55, P < 0.05 or P<0.01]. Conclusion Shenmai injection can elevate ATP, ADP, AMP, adenosine and creatinine contents in the cerebral cortex of rats, possibly that is the theoretical basis for brain protective mechanism of Shenmai injection.

19.
European J Med Plants ; 2014 Nov; 4(11): 1356-1366
Article in English | IMSEAR | ID: sea-164202

ABSTRACT

Aims: The present studies were initiated to develop a cost effective protocol for micropropagation, as a mean for conservation of medicinal plant- Tylophora indica (Burm f.) Merill. The plant is threatened and needs immediate conservation, therefore, the study was undertaken with following objective:  In vitro multiplication of Tylophora indica using nodal axillary bud proliferation and through organogenesis of callus. Study Design: For all experiments ten replicates were used per treatment and all the experiments were repeated three times. Data have been presented as Mean ± Standard deviation. Place and Duration of Study: Department of Plant Cell and Molecular Biology, Indian Institute of Advanced Research (IIAR), Gandhinagar, Gujarat, India. Methodology: For in vitro plant regeneration, micropropagation and organogenesis techniques were used. For micropropagation, surface sterilized nodal explants were inoculated on different shoot inducing media and further multiplication was obtained. Root containing shoots were transferred to pot containing pre autoclaved mixture of soil and soilrite. For organogenesis, surface sterilized leaf explants were inoculated on different types of callus inducing media. Results: All the nodal explants were sprouted at a very high frequency, i.e. 98% and sprouted buds elongated up to 8cm on three different media. Dissected explants grew further and average height of shoots reached 9.5cm±0.80cm within 30 days. Interestingly, root formation was observed on the same media; so that the best media was 0.4mg L-1 BA and 0.1mg L-1 Kn for both initiation as well as for multiplication. For organogenesis, the fragile callus was observed on media containing 2mg L-1 2,4-D and 0.1mg L-1 Kn. Green pigmented calli were transferred to MS media, where it regenerated in to shoots and roots, simultaneously Conclusion: The protocol of micropropagation through axillary bud proliferation described here is very simple, repetitive and cost effective, which can be easily utilized for commercial cultivation. On shoot multiplication media, root formation observed, thereby making the process is one step; which is very easy to follow.

20.
Article in English | IMSEAR | ID: sea-168186

ABSTRACT

Recent research has increased our knowledge and understanding of the antimicrobial effect of marine Streptomyces. In a systematic screening effect, Streptomyces isolates from different marine seaweeds were investigated for antimicrobial activity against Vibrio pathogens (V. harveyji, V. parahemolyticus, V. vulnificus and V. algniolyticus) and potentially active secondary metabolites. The different solvent extracts (Butanol, Ethyl acetate, Methanol, hexane) of selected isolates were screened for their antivibrio activity by disc method. The Zone of inhibition was measured in all extracts revealed a wide range of antimicrobial activity against pathogenic vibrios. The overall results of the antimicrobial activity indicates the abundance of purine riboside antibiotic in streptomycetes from marine seaweeds evidenced by the presence of precursor molecules such as guanosine, xanthosine in the extractions of Streptomyces which can be exploited for the production of purine riboside antibiotics which are in use of pharmaceutical industry.

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