ABSTRACT
Background:Urinalysis is a procedure for examining physical properties, particulate matter, cells, casts, crystals, organisms and solutes. Urinalysis is a routine screening and diagnostic tool used to identify infections including renal, urological, liver disease, diabetes mellitus and urinary tract infection (UTI). Urinalysis can be used to screen, glucose, leucocytes, nitrite and blood. Although urinalysis is an effective screening tool it should not be used in isolation to guide treatment because false positives, for example, nitrites, and false negatives, for example, glucose, can occur if the sample is contaminated or left to stand for too long. UTI is among the leading reasons for treatment in adult primary care medicine, accounting for a considerable percentage of antibiotic prescriptions. Aim of this study is to identify the markers of urinary tract infection by urine routine microscopic analysis in pregnant women.Methods:This was a two months prospective study from June 1st 2019 to July 31st 2019. Urine samples of 72 pregnant women coming routinely to OPD of GMC,Doda were examined by physicaland biochemical analysisby microscopy methods.Results:Majority of samples were pale yellow with turbid appearance. Protein was present in most of the samples whereas glucose was present in a minor number of samples. Microscopy revealed increase in pus and epithelial cells count in most of the samples while bacteria were present in almost half of the samples.Conclusions:To conclude from the present study that appearance, presence of protein, pus cells, epithelial cell and bacteria, all taken together can serve as a strong indicator of UTI.
ABSTRACT
Background: Urinary tract infection is diagnosed by the growth of many organisms of a single specimen in the urine with presence of many symptoms. bacteriuria is defined as growth with a colony of >105/ml of a single species in a midstream clean catch urine sample.Methods: A cross sectional study was undertaken in febrile children with urinary tract infection attending Department of Paediatrics, tertiary care hospital, Bangalore during the period January 2017 to December 2017.Results: Out of the 200 children studied, 93 children belonged to the age group of 6 year to 12 year and 57 children belong to 1 year to 6-year age group and 50 children belongs to 12 year to 18-year age group. 97 children showed significant pyuria in centrifuged urine sample of which 49 were males and 48 were females. Majority 45 children were belonging to age group of 6 year to 12 year. Out of 97 children showed significant pyuria in centrifuged urine sample of which 46 children 5-10 cells/HPF and 27 children showed > 10 cells/HPF and 24 cases showed < 5cells / HPF. Out of 200 children, in 29 cases urine culture showed E. coli growth and 51 cases showed no growth.Conclusions: Urinary tract infections are common in childhood. Nearly all UTIs are caused by bacteria that enter the opening of the urethra and move upward to the urinary bladder and sometimes the kidneys.
ABSTRACT
Background: New-born acquires mother’s microbiome from the birth canal during the delivery process and these bacteria then colonize in the gut. Studying the oral microbiome of infants offer a good perspective for us to understand the gut microbiome disruption caused by C-section. A previous study of three-month-old infants reported a higher amount of bacterial taxa of the oral microbiota in vaginally delivered infants than the ones with another birth mode. Aim: Comparison of microflora between the birth canal and the oral cavity of a newborn in Caesarean delivery. Materials and methods: 30 pregnant women selected for the study. Parturient canal samples obtained prior to delivery and infant’s oral cavity oral samples obtained immediately after birth. Samples transported to the lab for microbiological assays and data tabulated and statistically analyzed using Chi-Square Test. Results: E.coli was observed in 21.1% (N=7) in the vaginal swab whereas its occurrence in buccal smear was 10.3% (N=3). The chi-square test of independence was statistically insignificant (p> 0.05). The staphylococcus was observed in 44.8 (N= 13) of vaginal swab and 37.9% (N=11) of buccal smear and again the difference was statistically insignificant. The presence of pus cells was 31% (N=9) in vaginal swab and 10.3% (N=3) in the buccal smear. Conclusion: A significant number of mother-child pairs showed the presence of Staphylococcus aureus, Klebsiella, and E.coli. However, Klebsiella and pus cells were occasionally found.