Development And In Vitro Evaluation Of A New Topical O/W Emulgel From Fruit Extract Of Pyrus Communis

Objective: The aim of the present study was to develop a new topical dosage form containing Pyrus communis fruit extract. Developed formulation was O/W Emulgel which was evaluated by its in vitro tests and its stability studies at different storage conditions. Methods: Hydroalcoholic Pyrus communis extract was prepared by the maceration process. A 4% Pyrus communis emulgel was prepared by the combination of emulsion and gel at a specific temperature and mixing through homogenizers. The formulations having different concentration of carbopol 940 (gelling agent) were placed at 8 °C, 25 °C, 40 °C and 40 °C+75%RH for 3 mo in order to find out the most stable formulation. After the selection of final emulgel formulation was eventually further evaluated for in vitro studies such as phase separation, centrifugation, rheology, pH, conductivity, organoleptic properties and mean droplet size over a period of 12 w at 8 °C, 25 °C, 40 °C and 40 °C+75%RH. Results: In vitro evaluation of the selected Pyrus communis emulgel formulation showed good resistance to phase separation on centrifugation, conductivity gradually increases due to oil in water emulgel and pH of formulation was gradually decreased. The rheological behavior was non-Newtonian pseudoplastic and showed shear thinning fluid behavior. Mean droplet size of Pyrus communis emulgel was 16.0±0.20 µm and after 90 d droplet size was 16.7±0.55 µm at high storage temperatures at 40 °C and 40 °C+75RH and no significant changes were observed at normal storage conditions at 8 °C and 25 °C. Conclusion: Pyrus communis emulgel fresh fruit extract showed stable formulation at different storage conditions. This new formulation will be a good addition to pharmaceutical dosage forms made from traditionally used plants.

Aspergillus niger as the Source of Ochratoxin A of Contaminated Pyrus communis in Taif Market

Fruits are one of the most important agricultural products that supply the body with vitamins and essential minerals elements, but it is contaminated by fungi during the period of growth, harvesting and storage. A. niger is one of the species that grows on the fruit during the period of storage, and secretes mycotoxins especially ochratoxin A. This study was conducted with the purpose of isolating and identifying different strains of A. niger from 20 samples of pear collected from Taif markets and to determine the ability of these strains to produce OTA. It was observed that showed that out of 20 pear samples collected, 19 samples were detected to be contaminated with different strains of A. niger and the strains were able to produce OTA. From 27 isolates of A. niger which was used to test the ability of production OTA, 10 strains only produced OTA. The range of OTA in all strains were 0.18 to 9.5 ppb. Representative 27 strains of ochratoxigenic and non ochratoxigenic black Aspergilli isolated were subjected for detection of ochratoxin biosynthesis genes, by using two sets of primer for two genes involved in ochratoxin biosynthetic pathway. Bands of the fragments of PKS15C-MeT and PKS15KS genes visualized at 998 and 776 bp, respectively. Whereas, the presence of four tested genes is not sufficient marker for differentatin between aflatoxigenic and non aflatoxigenic isolates.

Temperature Effect on Traditional Pear (Pyrus communis L.) Landraces Seed Germination

Investigations included six traditional pear landraces (Pyrus communis L.): Aleksandra, Vodenka, Patlidjanka, Ljubichica, Tatlikuti and Crvenushka. The number of seeds per fruit, seed energy and total seed germination were investigated and statistically analysed (P = .05 and P = .01). Seed stratification encompassed pre-chilling by using three treatments: I treatment covers seed pre-chilling at -18°C for 24 hours; II treatment covers seed pre-chilling at +1°C for 2 weeks; III treatment covers seed pre-chilling at +8°C for 1 week. Only Patlidjanka belongs to the group with high seed number in the fruit. The other landraces produced low seed number like 2-3 seeds per fruit. The average number of all researched landraces is 3.67 seeds per fruit. The pear Tatlikuti showed a very high significant difference (P = .01). It was concluded that III treatment showed the highest seed energy at landraces Ljubichica 77.5% and Tatlikuti 75.0% and the lowest at Aleksandra 52.5%. There were significant differences (P = .05) between the applied treatments regarding seed energy. In II treatment, the total seed germination had the highest effect on Patlidjanka 87.75%. The values for germination seeds in the other landraces were in the range of 77.5% to 86.25%. Statistically, a significant difference was determined in the landraces Ljubichica and Crvenushka (P = .05), and there was high significant difference (P = .01) in the pear Aleksandra. The II and III treatments could be recommended as the most suitable to improve the pear seed germination.

Hybrid cultivars of pear in subtropics regions: processing ability in the form of jelly / Cultivares de peras híbridas em regiões subtropicais: aptidão para processamento na forma de geleia

ABSTRACT: The aim of this study was to characterize five different hybrid cultivars pears ('Tenra', 'Centenária', 'Cascatence', 'Primorosa' and 'Seleta') deployed in subtropical regions of Brazil regarding its physicochemical characteristics, bioactive compounds and antioxidant activity, as well as to evaluate the influence of these cultivars on the characteristics physicochemical, rheological properties and sensory acceptance of the obtained jelly, for identifying varieties with higher potential for industrial use. Regardingthe bioactive compounds and antioxidant activity it was observed that 'Seleta' cultivar was characterized by a higher phenolic content (57.68mg GAEs 100g-1), higher antioxidant activity (13.56% of DPPH sequestration and 1.25µM of trolox g -1) and higher content of vitamin C (3.59mg 100g-1). As the acidity, it ranged from 4.09 to 5.81 g of malic acid 100g-1 ('Tenra' and 'Seleta', respectively). Regarding color, the color parameter L* varied from 32.14 to 36.83, a* ranged from 2.87 to 5.20, b* ranged from 16.57 to 20.42, Chroma ranged from 16.67 to 21.02 and °Hue ranged from 74.94 to 80.18. Regarding the texture, the jellies produced from 'Centenária' and 'Tenra' were characterized by having the highest values of hardness (1.21 and 1.23N, respectively), gumminess (0.42 and 0.45, respectively) and chewiness (0.40 and 0.45N, respectively). The jelly obtained from the 'Centenária' still showed the highest adhesiveness (2.27N s-1) and springiness (0.99). Already jellies prepared by 'Cascatence' showed the highest cohesiveness values (0.68). The jellies prepared with different pear cultivars showed good sensory acceptance for all measured properties, with average scores ranging between the hedonic terms "liked slightly" to "liked very much". Cultivars of subtropical pear trees present different physicochemical characteristics, resulting in jellies with different physicochemical characteristics and rheological properties. Processing in the form of jelly is extremely viable due to the good acceptance by consumers of all formulations elaborated.

RESUMO: O objetivo deste estudo foi caracterizar cinco diferentes cultivares híbridas de peras ('Tenra', 'Centenária', 'Cascatence', 'Primorosa' e 'Seleta') implantadas em regiões subtropicais do Brasil quanto as caracteristicas físico-química, compostos bioativos e atividade antioxidante, bem como avaliar a influência destas cultivares sobre as características físico-químicas, propriedades reológicas e aceitação sensorial da geleia obtida, para identificação de cultivares com maior potentcial para a utilização industrial. Com relação aos compostos bioativos e atividade antioxidante, pode-se observar que a cultivar 'Seleta' caracterizou-se pelo maior teor de fenólicos (57,68mg GAEs 100 g-1), maior atividade antioxidante (13,56% de sequestro de DPPH e 1,25μM de trolox g-1) e se destaca devido ao seu maior teor de vitamina C (3,59mg 100g-1). A acidez, variou de 4,09 a 5,81g de ácido málico 100 g-1 ('Tenra' e 'Seleta', respectivamente). Em relação à cor, o parâmetro de cor L* variou de 32,14 a 36,83, a* variou de 2,87 a 5,20, b* variou de 16,67 a 20,42, Chroma variou de 16,57 a 21,02 e °Hue variou de 74,94 a 80,18. Em relação à textura, as geleias produzidas a partir de 'Centenária' e 'Tenra' caracterizam-se por apresentar os maiores valores de dureza (1,21 e 1,23N, respectivamente), gomosidade (0,42 e 0,45N, respectivamente) e mastigabilidade (0,40 e 0,45, respectivamente). A geleia obtida da 'Centenária' ainda apresentava a maior adesividade (2,27N s-1) e a elasticidade (0,99). Já as geleias preparadas com a 'Cascatence' apresentaram os maiores valores de coesividade (0,68). As geleias preparadas com diferentes cultivares de pera mostraram boa aceitação sensorial para todas as propriedades avaliadas, com notas médias variando entre os termos hedônicos "gostei ligeiramente" e "gostei muito". As cultivares de pereiras subtropicais apresentam características físico-químicas diferentes, resultando em geleias com características físico-químicas e propriedades reológicas distintas. O processamento sob a forma de geleia é extremamente viável devido à boa aceitação por parte dos consumidores de todas as formulações elaboradas.

Compatibilidade fenotípica entre cultivares de pereiras europeias eporta-enxertos de marmeleiro / Phenotypic compatibility among european pear cultivars and quince rootstocks

A cultura da pereira (Pyrus communisL.) possui grande potencial de expansão no sul do Brasil devido às condições climáticas e de solo. A carência de informações a respeito da(s) melhor(es) combinação(ões) de cultivares 'copa de pereiras europeias' e porta-enxertos de marmeleiro em relação à compatibilidade de enxertia é um dos fatores que tem limitado o cultivo de pera no país. A utilização de porta-enxertos de marmeleiros apresentam as vantagens de redução do vigor e rápida entrada em produção, porém, quando enxertados com algumas cultivares de 'pereira europeia', pode acarretar incompatibilidade de enxertia. O trabalho foi realizado com o objetivo de avaliar fenotipicamente a compatibilidade morfológica entre cultivares 'copa de pereiras europeias' e porta-enxertos de marmeleiro através das variáveis de crescimento das plantas, como o incremento de diâmetro (mm) do tronco da cultivar e do porta-enxerto; diferença de diâmetro entre a cultivar e o porta-enxerto; incompatibilidade 'translocada'; incompatibilidade 'localizada'; e análise da conexão vascular no ponto de enxertia pela imersão em solução corante de Ácido Fuccínico 0,08%. Os experimentos foram conduzidos em pomares de pereiras europeias na região de Urupema, estado de Santa Catarina, durante os ciclos de cultivo 2011/12, 2012/13 e 2013/14. As combinações de cultivares de pereiras europeias e porta-enxertos avaliados foram a 'Abbè Fetel'/'Adams', 'Decana du Comice'/'Adams', 'Clapp's Favourite'/'EMA', 'Rocha'/'Adams', 'Packham´s Triumph'/'EMA' e 'Santa Maria'/'Adams'. A combinação 'Clapp´s Favourite'/'EMA' apresentou incompatibilidade do tipo 'localizada' pela descontinuidade vascular na região de união do enxerto, impedindo a passagem do corante. As combinações 'Rocha'/'Adams' e 'Abbè Fetel'/'Adams' não apresentaram incompatibilidade de enxertia. Nessas combinações, foram observadas união vascular contínua entre as cultivares e os porta-enxertos. As combinações ...

The pear (Pyrus communis L.) crop offers an expansive market opportunity in southern Brazil, because of amicable climatic and soil conditions. However, the lack of studies on compatibility of european pear cultivars and rootstocks is one of the limiting factors on the development of the pear crop in southern Brazil. The use of quinces as rootstocks has the advantages of vigor reduction and earlier bearing trees. However, some of these combinations can present some incompatibility graft. The objective of this study was to evaluate phenotypicaly the morphological compatibility among european pear cultivars and quince rootstocks. The evaluated variable were the section increment of cultivars and rootstocks trunk diameter at the graft union; the diameter difference between scion and cultivars and rootstocks; 'translocated' incompatibility; 'located' incompatibility; and the vascular connection of the graft union by immersion of the base of the plants (under the graft union) in a 0.08% Fuccinic acid solution. The experiments were conducted in an european pear orchards in the region of Urupema municipality, state of Santa Catarina, during the 2011/12, 2012/13 and 2013/14 growing seasons. The european pear cultivars and rootstocks evaluated were: 'Abbé Fetel'/'Adams', 'Decana du Comice'/'Adams', 'Clapp's Favourite'/'EMA', 'Rock'/'Adams', 'Packham's Triumph'/'EMA' and 'Santa Maria'/'Adams'. 'The Clapp´s Favourite'/'EMA' combination showed a `located incompatibility by descontinued vascular graft region, preventing the dye translocation. 'The Rocha'/'Adams' and 'Abbè Fetel'/'Adams' combinations did not present graft incompatibility, showing continued vascular union and no vascular disruption between cultivars and rootstocks. The 'Santa Maria'/'Adams', 'Decana du Comice'/'Adams' and 'Packham´s Triumph'/'EMA' combinations showed a good graft union region with clear and continued bark and wood bond lines. The 'Clapp´s Favourite'/'EMA' ...

Produção e qualidade pós-colheita de cultivares de pereira nas condições subtropicais da região leste paulista / Production and postharvest quality of pear tree cultivars in subtropical conditions at eastern of São Paulo state, Brazil

Objetivou-se avaliar o desempenho produtivo e a qualidade pós-colheita de cultivares de pereira nas condições subtropicais da região leste paulista. Para tanto, um ensaio de competição de cultivares foi implantado no município de Jundiaí-SP, com cinco cultivares ('Centenária', 'Triunfo', 'Primorosa', 'Seleta' e 'Tenra'). O experimento foi implantado em 2007, no espaçamento de 3,0mx4,0m, com mudas enxertadas no porta-enxerto Pyrus calleryana. As avaliações foram realizadas em três ciclos vegetativos (2009/10, 2010/11 e 2011/12), analisando-se os aspectos fenológicos, produtivos e a qualidade pós-colheita das frutas. A pereira 'Tenra' apresentou a maior produção, com 6kg de frutos por planta. As cultivares estudadas produziram frutas com coloração típica e firmeza aceitável, porém pouco doces.

This research aimed to evaluate the production and postharvest quality of pear tree cultivars in subtropical conditions at eastern of São Paulo state. For such, an assay of competition among cultivars was implanted in the Jundiaí city-SP, with five cultivars ('Centenária', 'Triunfo', 'Primorosa', 'Seleta' and 'Tenra'). The experiment was implanted in 2007, the spacing was of 3.0mx4.0m, and the plants were grafting in Pyrus calleryana. The evaluations had been carried through in three vegetative cycle (2009/10, 2010/11 and 2011/12), the phenological stage, productive aspects and fruit postharvest quality were evaluated. The pear 'Tenra' presented highest production, with 6kg of fruits per plant. The cultivars produced fruits with typical coloring firmness and acceptable, but slightly sweet.

Influence of rootstocks on growth, yield, fruit quality and leaf mineral element contents of pear cv. 'Santa Maria' in semi-arid conditions

BACKGROUND: Rootstocks play an essential role to determining orchard performance of fruit trees. Pyrus communisand Cydonia oblonga are widely used rootstocks for European pear cultivars. The lack of rootstocks adapted to different soil conditions and different grafted cultivars is widely acknowledged in pear culture. Cydonia rootstocks (clonal) and Pyrus rootstocks (seedling or clonal) have their advantages and disadvantages. In each case, site-specific environmental characteristics, specific cultivar response and production objectives must be considered before choosing the best rootstock. In this study, the influence of three Quince (BA 29, Quince A = MA, Quince C = MC) and a local European pear seedling rootstocks on the scion yield, some fruit quality characteristics and leaf macro (N, P, K, Ca and Mg) and micro element (Fe, Zn, Cu, Mn and B) content of 'Santa Maria' pear (Pyrus communis L.) were investigated. RESULTS: Trees on seedling rootstock had the highest annual yield, highest cumulative yield (kg tree(-1)), largest trunk cross-sectional area (TCSA), lowest yield efficiency and lowest cumulative yield (ton ha(-1)) in the 10th year after planting. The rootstocks had no significant effect on average fruit weight and fruit volume. Significantly higher fruit firmness was obtained on BA 29 and Quince A. The effect of rootstocks on the mineral element accumulation (N, K, Ca, Mg, Fe, Zn, Cu, Mn and B) was significant. Leaf analysis showed that rootstocks used had different mineral uptake efficiencies throughout the early season. CONCLUSION: The results showed that the rootstocks strongly affected fruit yield, fruit quality and leaf mineral element uptake of 'Santa Maria' pear cultivar. Pear seedling and BA 29 rootstock found to be more prominent in terms of several characteristics for 'Santa Maria' pear cultivar that is grown in highly calcareous soil in semi-arid climate conditions. We determined the highest N, P (although insignificant), K, Ca, Mg, Fe and Cu mineral element concentrations on the pear seedling and BA 29 rootstocks. According to the results, we recommend the seedling rootstock for normal density plantings (400 trees ha(-1)) and BA 29 rootstock for high-density plantings (800 trees ha(-1)) for 'Santa Maria' pear cultivar in semi-arid conditions.

Cultivares e portaenxertos sobre o vigor de plantas de pereira europeias / Cultivars and rootstocks on plants vigor of European pear

A cultura da pereira (Pyrus communis) possui grande potencial de expansão no sul do Brasil devido às condições climáticas e solo. A carência de informações a respeito da(s) melhor(es) combinação(ões) de cultivares copa de pereira europeia e portaenxertos, quanto ao aspecto vegetativo e potencial produtivo, tem limitado o cultivo. O objetivo do trabalho foi avaliar a influência de cultivares copa de pereira europeia e portaenxertos de marmeleiro (Cydonia oblonga) sobre o vigor e variáveis de plantas. Os experimentos foram conduzidos em pomares comerciais da cidade de Fraiburgo, Estado de Santa Catarina, durante os ciclos de cultivo 2008/09, 09/10 e 10/11. As cultivares avaliadas foram 'Packham's Triumph', 'Santa Maria', 'Rocha' e 'Abbè Fetel' e os portaenxertos de marmeleiro EMC e Adams. O espaçamento de plantio foi de 0,3m entre plantas e 4m entre linhas. As variáveis analisadas foram: a. incremento de altura de plantas (m); b. incremento de volume de copa (m³); c. incremento de diâmetro do tronco da cv. 'copa' (mm); d. incremento de diâmetro do tronco do portaenxerto (mm); e. diferença de diâmetro do tronco entre a cultivar copa e o portaenxerto (mm); f. índice de fertilidade (no de gemas cm-1); g. massa fresca média total acumulada do material vegetativo das três podas de inverno (kg); h. diferença cumulativa entre a massa fresca e a massa seca dos ramos podados (kg). As diferentes combinações de cultivares e portaenxertos interferiram nas diferentes variáveis analisadas. As combinações Abbè Fetel e Rocha sobre marmelo Adams, foram significativamente mais vigorosas em termos de incremento de diâmetro do tronco da cultivar copa, incremento de altura de plantas, incremento de volume de copa e massa fresca média total acumulada do material vegetativo das podas de inverno dos três anos. A combinação Santa Maria sobre marmelo Adams apresentou vigor intermediário significativo de plantas de acordo com o incremento em volume de copa, diferença de diâmetro do tronco entre a cultivar copa e do portaenxerto, sendo mais indicada para uso comercial, devido ao maior equilíbrio proporcionado entre parte vegetativa e produtiva da planta, nas condições edafoclimáticas experimentais do Sul do Brasil.

The pear (Pyrus communis) culture offers a great expansive market opportunity in southern Brazil, according to climatic conditions and soil. However, there are still some barriers that prevent satisfactory economic production, such as lack of knowledge about the best combinations of cultivars and rootstocks. The objective of the study was to evaluate the influence of European pear cultivars and quince (Cydonia oblonga) rootstocks on the plants vigor variables. The experiments were carried out in commercials orchards in Fraiburgo Municipality, Santa Catarina state, during the crop season of 2008/09, 09/10 and 10/11. The cultivars evaluated were 'Packham's Triumph', 'Santa Maria', 'Rocha' and 'Abbè Fetel' and the quince rootstocks EMC and Adams. The cultivars and rootstocks combinations were conducted in a spacing of 0.3mx4m between plants and rows, respectively. The vigor variables evaluated were, a. plants height increment (m); b. canopy volume increment (m³); c. truck diameter increment of the cultivars canopy (mm); d. truck diameter increment of rootstocks (mm); e. difference between the trunk diameter of cultivars canopy and rootstock (mm); f. fertility index (buds cm-1); g. total cumulative average of fresh weight of pruned branches (kg); and h. cumulative difference between the fresh and dry weight of pruned branches (kg). All combinations evaluated affected the vegetative parameters of the European pear cultivars. The combinations Abbè Fetel and Rocha grafted on Adams quince rootstocks are significant more vigorous according to increment of cultivars canopy truck diameter, plant height increment, cultivars canopy volume increment and cumulative average weight of pruned branches, when compared with others combinations. The combination Santa Maria grafted on Adams quince rootstock showed significant intermediate vigor according to cultivar canopy volume increment, truck diameter of cultivar canopy and, truck diameter of rootstock. This combination presented a better proportion amongst the vigor variables and can be indicated for commercial production according to the experimental edafoclimatic conditions of southern Brazil.

Aumento da produção de pereira asiática pelo uso de fitorreguladores / Fruit production increase in Asian pear trees by use of plant growth regulators

A baixa frutificação de pereiras nas condições do Sul do Brasil é um dos limitantes à produção da cultura no país, podendo o problema ser minimizado pelo uso de fitorreguladores. Objetivou-se avaliar a efetividade de diferentes fitorreguladores e a combinação dessas substâncias no aumento da frutificação de pereira asiática 'Shinseiki. A aplicação dos fitorreguladores foi realizada quando as pereiras atingiram o estádio de plena floração. O delineamento experimental foi em blocos casualizados, com quatro repetições de uma planta, sendo avaliados os seguintes tratamentos: 1. testemunha (sem aplicação); 2. thidiazuron (TDZ) 20mg L-1; 3. ácido giberélico (AG) 20mg L-1; 4. proexadione cálcio (PCa) 600mg L-1; 5. PCa 600mg L-1 + TDZ 20mg L-1; 6. PCa 600mg L-1 + AG 20mg L-1; e 7. AG 20mg L-1 + TDZ 20mg L-1. Os fitorreguladores thidiazuron e ácido giberélico, e a combinação dessas substâncias, ambos na concentração de 20mg L-1, quando aplicados na floração, determinam aumento significativo da frutificação e da produção de peras 'Shinseiki'. A utilização do proexadione cálcio na floração, tanto isoladamente quanto associado ao thidiazuron e ao ácido giberélico, não repercutiu no aumento da frutificação.

The low fruit set in the Southern Brazil conditions is one of the limiting factors to pear production in the country. The use of plant growth regulators may minimize this problem. This study was carried out aiming to evaluate the effectiveness of different growth regulators and the combination of these substances on fruit set increase of 'Shinseiki Asian pears. The application of growth regulators was performed when the pears have reached the full bloom stage. The experimental design was randomized blocks with four replications of one tree, with the following treatments: 1. control (no application); 2. thidiazuron (TDZ) 20mg L-1; 3. gibberellic acid (GA) 20mg L-1; 4. prohexadione calcium (PCa) 600mg L-1; 5. PCa 600mg L-1 TDZ + 20mg L-1; 6. PCa 600mg L-1 + AG 20mg L-1; and 7. AG 20mg L-1 + TDZ 20mg L-1. When sprayed on full bloom stage, thidiazuron and gibberellic acid, and combinations of these substances, both at a concentration of 20mg L-1, increased significantly the fruit set and the fruit production of 'Shinseiki' pears. The use of prohexadione calcium, single or in combination with thidiazuron and gibberellic acid, did not increase the fruit production.

Influence of cultivar and rootstock on early growth and syllepsis in nursery trees of pear (Pyrus communis L., Rosaceae)

A two-year field study was conducted to examine the capacity of spontaneous formation of long sylleptic shoots (LSS) in nursery trees of pear cvs. 'Abbé Fétel', 'Conference' and 'Starking Delicious' grafted on Quince MA (MA) and Quince BA 29 (BA 29) rootstocks in a nursery during the first year after bud grafting. Tree height (TH), trunk diameter (TD) - 10 cm above the bud union and number of LSS were measured at the end of each season. The TH was measured from the ground level. The highest number of LSS was developed by cv. 'Abbé Fétel' in both the seasons, and the lowest by cv. 'Starking Delicious'. Tree height and TD were highly significantly affected by the cultivar in both the years and by the rootstock in 2008. The interactions between them did not significantly affect the examined parameters. The study showed that the early growth and syllepsis of pear nursery trees during the first year after bud-grafting were incomparably more affected by the cultivar than by the rootstock under similar weather conditions and on the same soil in a crop rotation system.

Revestimento ativo de amido na conservação pós-colheita de pera Williams minimamente processada / Starch-based edible coating on extending shelf life of fresh-cut pear

A pera é uma das frutas de clima temperado mais consumidas no Brasil, no entanto sua conservação é limitada devido ao seu escurecimento quando sofre danos ou tratamentos físicos. Os revestimentos comestíveis interagem favoravelmente com o alimento, aumentando sua vida de prateleira. Este trabalho objetivou avaliar a ação de revestimento de amido adicionado de lactato de cálcio e L-cisteína na inibição do escurecimento enzimático, na redução do crescimento de psicrotrófilos e enterobactérias e na manutenção da textura. As peras fatiadas foram cobertas com revestimentos comestíveis à base de amido incorporados com cisteína e lactato de cálcio, exceto o controle - sem revestimento (C). Os tratamentos foram: apenas revestimento (T1); 2,0 por cento lactato de cálcio e 1,0 por cento L-cisteína (T2) e 2,0 por cento lactato de cálcio e 1,5 por cento L-cisteína (T3). As amostragens foram feitas nos tempos zero, dois, quatro e seis dias. As amostras foram mantidas sob refrigeração (7°C±2°C). As peras submetidas aos tratamentos T2 e T3 apresentaram-se significativamente mais firmes (P<0,05) que as dos tratamentos C e T1. A ação da cisteína sobre a inibição do escurecimento enzimático foi observada nos tratamentos T2 e T3, que não apresentaram diferença significativa (P≥0,05) entre si para os valores de ∆E, no entanto esses valores foram significativamente menores em relação ao controle. No tempo 6, a redução decimal na contagem de psicrotrófilos chegou a 3,03 e 2,43 para T3 e T2, em comparação com o controle. A contagem de enterobactérias apresentou comportamento semelhante, sendo o valor da redução de 3,16 e 3,05 para T2 e T3 em relação ao controle. Verificou-se que a vida de prateleira de pera minimamente processada pode ser mantida por mais tempo com o uso desse revestimento.

Pear is one of the most consumed temperate fruits in Brazil; however their conservation is limited due to browning when it suffers injuries or physical treatments. The edibles coating interact with the food positively extending its shelf life. This research aimed to evaluate the action of starch edible coating incorporated with calcium lactate and L-cysteine on enzymatic browning inhibition, on psychrotrophs and enterobacteriaceae growing reduction and on firmness maintenance. The sliced pears were coated with starch edible coating incorporated with L-cysteine and calcium lactate, except control, without coating (C). The treatment were: only coating (T1); 2,0 percent calcium lactate and 1,0 percent L-cysteine (T2); 2,0 percent calcium lactate and 1,5 percent L-cysteine (T3). The samples were taken at 0, 2, 4 and 6 days. Pears were keeped under refrigeration (7°C±2°C). Pears submitted to treatments T2 and T3 show significantly more firms (P<0,05) compared to treatments C and T1. Cysteine action over enzymatic browning inhibition was observed in treatments T2 and T3 which do not differed significantly each other (P≥0,05) to ∆E values however these were significantly lower than control (C). At time 6, decimal reduction on psychrotrophs counting reached 3,03 and 2,43 to T3 e T2 compared to control. Enterobacteriaceae counting showed similar behavior where the reduction values were 3,16 and 3,05 to T2 e T3 compared to control. It was verified that using the studied edible coating on fresh cut pear can extend its shelf life.

Multiplicação fotoautotrófica in vitro de pereiras 'Abate Fetel' / Photoautotrophic multiplication in vitro of 'Abate Fetel' pears

Novas cultivares de pereira têm sido introduzidas no Brasil, como a 'Abate Fetel'. A produção de mudas de qualidade dessa cultivar é necessária, sendo possível obter tal cultivar pelo uso de técnicas de micropropagação. Objetivou-se avaliar diferentes concentrações de sacarose e tipos de vedação dos frascos na multiplicação in vitro de pereiras 'Abate Fetel'. Segmentos nodais contendo de duas a três gemas axilares, de aproximadamente 1cm de comprimento, oriundos de plantas pré-estabelecidas in vitro, foram inoculados em meio de cultura 'QL', suplementados com mio-inositol (100mg L-1), sacarose (0, 15, 30, 45g L-1), BAP (1mg L-1), AIB (0,1mg L-1) solidificado com 6,5g L-1 de ágar e pH ajustado para 5,7. Os frascos foram vedados com algodão, alumínio ou filme plástico de PVC e mantidos em sala de crescimento a 25±2°C, com intensidade luminosa de 42µmoles m-2 s-1 e fotoperíodo de 16 horas. Os frascos vedados com algodão apresentaram o maior número de folhas na ausência de sacarose, não sendo observado efeito das concentrações de sacarose presentes no meio de cultura sobre o número de brotações e número de folhas formadas. O comprimento médio das brotações e a massa fresca total não diferiram entre os tipos de vedação empregados, porém observou-se aumento expressivo dessas variáveis frente ao aumento da sacarose no meio de cultura. A vedação dos frascos com filme de PVC proporcionou o maior número de brotações por explante quando utilizadas altas concentrações de sacarose.

New pear cultivars have been introduced in Brazil as the 'Abate Fetel'. Seedling production with higher quality of this cultivar is needed, and this can be obtained by use of micropropagation techniques. The objective of the present study was to evaluate different sucrose concentrations and types of closure flasks on micropropagation of 'Abate Fetel' pears. Nodal segments with two to three axillary buds, about 1 cm in length, from plants pre-established in vitro, were inoculated on 'QL' culture medium supplemented with myo-inositol (100mg L-1), sucrose (0, 15, 30, 45g L-1), BAP (1mg L-1), IBA (0.1mg L-1) solidified with 6.5g L-1 agar and pH adjusted to 5.7. The flasks were closured with cotton, aluminum or PVC plastic film and kept in a growth chamber at 25±2°C, light intensity of 42µmoles m-2 s-1 and 16h of photoperiod. The flasks closured with cotton showed the highest number of leaves formed in the absence of sucrose and it was not observed effect of sucrose concentration in the culture medium of shoots number and number of leaves formed. The average shoot length and the total fresh weight did not differ between the types of closure flasks used, but there was a significant increase of these variables against the sucrose increase in the culture medium. The closure flasks with PVC film showed the highest number of shoots per explant, when high sucrose concentrations was used.