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Objective: To qualitative analze the chemical components in rat lung tissues after oral administration Qinbai Qingfei Concentrated Pellets (QQCP). Methods: The rat lung tissues were collected and analyzed by UPLC-Q-TOF-MS after ig administration of QQCP. The chemical components in control and dose groups were identified and speculated by Peakview and Metabolite Pilot data processing software using retention time, exact relative molecular mass, and cleavage fragments of MS/MS as indexes. Results: A total of 25 compounds were identified, including 17 prototypes and eight metabolites. Conclusion: The chemical constituents in the rat lung tissues were identified after oral administration of QQCP based on UPLC-Q-TOF-MS, which will contribute to elucidate the effects of potential pharmacodynamic material basis of QQCP on mycoplasma pneumoniae.
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Objective: To study the mechanism of Qinbai Qingfei Concentrated Pellets (QQCP) in the treatment of mycoplasma pneumonia pneumonia. Methods: UPLC-Q-TOF-MS was applied to detect the change of endogenous substances in the lung tissue of mycoplasma pneumonia mice model after taking decoction of QQCP orally. Progenisis QI was adopted to identify and match the peak, and principal compoment analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to reduce data dimension. Through the analysis of tandem mass spectrum data of compound that had larger contribution to the separation among different groups (VIP > 1, P < 0.05) and the HMDB database retrieval to identify potential biomarkers. Results: Twenty potential biomarkers were identified from lung tissue as retinal, all-trans-retinoic acid, pyroglutamic acid, leukotriene C4, vitamin A, arachidonic acid, and prostaglandin I2. Compared with model group, QQCP group had callback function of 20 potential biomarkers. Conclusion: QQCP play a role of treatment for mycoplasma pneumonia by affecting retinol metabolism, linoleic acid, and arachidonic acid metabolism. These results indicated that QQCP had the characteristics of multi-pathway, multi-target and overall regulation in the treatment of mycoplasmal pneumoniae pneumonia.
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To analyze the main components of Qinbai Qingfei concentrated pellets in rat serum with UPLC-Q-TOF-MS technology and serum pharmacochemistry theory. After gavage administration with Qinbai Qingfei concentrated pellets, blood was collected from hepatic portal vein. ACQUITY UPLC BEH C₁₈(2.1 mm×100 mm, 1.7 μm) was used, with 0.1% formic acid agueous solution(A)-0.1%formic acid and acetonitrile(B) as the mobile phase for gradient elution. The flow rate was 0.3 mL•min⁻¹, the column temperature was maintained at 35 ℃. Through the comparative analysis fingerprints of Qinbai Qingfei concentrated pellets, drug containing-serum and blank serum, and with the help Peakview and Metabolitepilot software, components in serum were defined. A total of 28 compounds were identified, including 18 prototypes and 10 metabolites. As a result, UPLC-Q-TOF-MS technology and serum pharmacochemistry theory were applied to comprehensively expound Qinbai Qingfei concentrated pellets'constituents migrating to rat serum, and provide scientific basis for further studies for in vivo metabolic process and effective material base.
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To discuss the repair mechanism of Qinbai Qingfei concentrated pellets to AEC-Ⅱ of rats infected by mycoplasma through the observation of the changes and distribution of TGF-β and SP-A in lungs, totally 60 Wistar rats that weighing 80-100 g were collected, with male and female in half. The rats were divided into six groups randomly, with 10 rats each group, namely blank group, model group, positive group and Qinbai Qingfei concentrated pellets high, middle and low dose groups. Rats were infected through nasal intubation drip of MP. After 10 days of administration, serum and bronchoalveolar lavage fluid (BALF) were collected to detect the concentration of surface activity related protein A (SP-A) by ELISA, left pulmonary tissues of rats were collected to observe the expression and distribution of transforming growth factor-beta (TGF-β) and SP-A by immunohistochemistry, and right pulmonary tissues were taken to detect TGF-β and SP-A mRNA expression level by real-time quantitative PCR (RT-PCR). Qinbai Qingfei concentrated pellets can reduce the expression of TGF-β and increase the expression of SP-A in the lung tissues of rats infected by mycoplasma. Specifically, TGF-β was mainly distributed among the lung interstitium, while SPAs were mainly distributed in AEC-II and parts of alveolar macrophage. The level of SP-A was reduced in serum and increased in BALF in rats in Qinbai Qingfei concentrated pellets groups. It was proved that Qinbai Qingfei concentrated pellets can restore the normal morphology and function of the lung by reducing the content of TGF-β to inhibit epithelial-mesenchymal transition (EMT) of alveolar type II epithelial cells and increasing the expression of SP-A. Qinbai Qingfei concentrated pellets have the repairing ability to capillary vessel damage caused by MP in lung tissues of rats.
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AIM: To observe the inhibitory effects of the serum containing drugs of Qinbai Qingfei Concentrated Pellets(S-QQCP) on influenza A virus. METHODS: MDCK cells were cultured with the technology of cell culture in vitro,serum contained QQCP of low-,moderate-,high dosages were made with Serum Pharmacological methods,in order to study the action of S-QQCP inhibit the biosynthesis,absorption and direct destruction of influenza A virus. RESULTS: Compared with the controlled blank serum,different dosages of S-QQCP had significant inhibitory effects on the biosynthesis of influenza A virus(P0.05). CONCLUSION: S-QQCP can inhibit the biosynthesis and absorption of influenza A virus to a certain extent.