ABSTRACT
OBJECTIVE@#To investigate the impact of Qing'e Pill (, QEP) on the cancellous bone microstructure and its effect on the level of β-catenin in a mouse model of postmenopausal osteoporosis.@*METHODS@#Ninety-six 8-week-old specific pathogen free C57BL/6 mice were randomly divided into 4 groups (24/group): sham, ovariectomised osteoporosis model, oestradiol-treated, and QEP-treated groups. Three months after surgery, the third lumbar vertebra and left femur of the animals were dissected and scanned using micro-computed tomography (micro-CT) to acquire three-dimensional (3D) parameters of their cancellous bone microstructure. The impact of ovariectomy, the effect of oestradiol and QEP intervention on cancellous bone microstructure, and the expression of β-catenin were evaluated.@*RESULTS@#The oestradioland the QEP-treated groups exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickneßs, bone surface to bone volume ratio (BS/BV), and β-catenin expression compared with those of the model group (P <0.05). In contrast, the structure model index, trabecular separation, and BS/BV were significantly decreased compared with those of the ovariectomised osteoporosis model group (P <0.05). No differences were observed in the above parameters between animals of the QEP- and oestradiol-treated groups.@*CONCLUSIONS@#The increased β-catenin expression may be the mechanism underlying QEP's improvement of the cancellous bone microstructure in ovariectomised mice. Our findings provide a scientific rationale for using QEP as a dietary supplement to prevent bone loss in postmenopausal women.
ABSTRACT
AIM:T observe the effects of Modified Qing'e Pill on glucolipid metabolism and myocardium function in type 2 diabetic rats.METHODS:Seventy-two healthy similar weighted SD rats were randomly divided into six groups:the normal group,the model group,low dose group (2.7 g/kg),middle dose group (5.4 g/kg),high dose group (10.8 g/kg) and Metformin group (0.15 g/kg).Model of Diabetes mellitus was set by "high fat diet" + " intraperitoneal injection of streptozotocin (STZ) " All groups received intragastric administration once per day for eight weeks.Groups of Modified Qing'e Pill and Metformin group were given the corresponding dose of drugs,while the normal and model groups were given the same volume of saline.At the fixed time points (pre-administration,the 4th week,the 8th week),the mental state,the weight of the rats,the FBG and 2PBG of rats were observed and detected;2 h after the end of administration,relevant indicators (HbA1 c,LDL,HDL,TG,TC,hs-CRP,TNF-α) in serum and the relevant indicators (MDA,SOD,GSH) in myocardial tissue were determined.RESULTS:Compared with the model group,mental state,weight,blood glucose and lipid levels were all improved of rats in the administration group.Also,Modified Qing'e Pill could reduce the levels of hs-CRP and TNF-α in serum,increase the content of SOD and GSH in myocardium and decrease the content of MDA in myocardium.CONCLUSION:Modified Qing'e Pill can improve the glucose and lipid metabolism in type 2 diabetic rats,and protect myocardial tissue from injury through anti-inflammation and antioxidation.