ABSTRACT
Objective Attempting to create a chemical diversity" combinatorial library consisting of active components of Chinese materia medica compounds. Methods This paper was based on the theory of "thinking and method of 'combinatorial screening' of Chinese material medica compound", using the Qingluo Tongbi Decoction (QTD) which has exact effect against rheumatoid arthritis as system. Re-combination group of the formulation was used and various industrial separation techniques, such as resin and membrane, were used to replace the diversity of combinatorial chemistry synthetic route. Different products were used to establish combinatorial library using "Building Block". Results Different contents of active components in each "Building Block" were found during the establishment of combinatorial libraries. AB-8 resin had good adsorption and desorption functions on sinomenine, and good refined effects on saponin as well. The contents of kirenol were different in each "Building Block" of different combinations. Each "Building Block" had a relative high content of total flavonoids. Conclusion Different combinations and separation techniques lead the differences in types and contents of chemical constituents. So certain pharmacodynamic experiments are needed to evaluate the preparation and to filter out the relevant active "Building Blocks".
ABSTRACT
Objective To study the fouling mechanism during the filtration of traditional Chinese medicine decoction by inorganic ceramic membrane so as to make a valuable exploration both on membrane fouling prevention and the industrialization of membrane separation technology in the field of Chinese materia medica. Methods Micro-filtrating Qingluo Tongbi Decoction using alumina ceramic membrane, researching the applicability of the membrane with different pore sizes, observing the fouling on membrane surface by scanning electron microscopy, studying the resistance distribution of membrane in filtration process, analyzing the fouling existence shape, position, and forming rule. Results In this system, alumina membrane with the pore size of 0.2 ?m was most suitable, which had the biggest permeating flux and reservation rate of effective component with less fouling. Among the alumina membranes with three different pore sizes, auto resistance and pore block resistance made up inferior percentage in total resistance, fouling resistance distribution mainly consisted of the reversible resistance, such as concentration polarization and gel layer resistance. Conclusion Optimizing parameters of the techniques in membrane process, using suitable pretreatment method and cleaning method can reduce membrance fouling and make the memberane property recover.