ABSTRACT
Objective: To study the quality differences of medical material, raw decoction pieces, and processed products of Astragalus membranaceus var. mongholicus. Methods: The raw decoction pieces and processed products were obtained from genuine medicinal materials of A. membranaceus var. mongholicus. The reasons for the quality differences were analyzed by comparing the contents of astragaloside IV, calycosin-7-O-β-D-glucopyranoside, formononetin, total polysaccharides, saponins, and flavones. Results: With content analysis, the sequence was found as follows: astragaloside IV (medical material > raw decoction piece > honey-fried piece > alcohol-fried piece > salt-fried piece > fried piece); calycosin-7-O-β-D-glucopyranoside, formononetin, and total polysaccharides (medical material > raw decoction pieces > alcohol-fried piece > salt-fried piece > honey-fried piece > fried piece), total flavones (medical material > alcohol-fried piece > raw decoction pieces > salt-fried piece > honey-fried piece > fried piece), total saponins (medical material > honey-fried piece > raw decoction pieces > alcohol-fried piece > salt-fried piece > fried piece). Conclusion: The temperature and supplementary material may play the main roles for quality differences of A. membranaceus var. mongholicus.
ABSTRACT
The HPLC fingerprint differences ofRadix Platycodonis from different origins were studied to provide references for their quality control and production. The total platycodins were purified by DB101 macroporous resin. HPLC-ELSD fingerprints of the total platycodins for 39 batches ofRadix Platycodonis samples from 9 provinces were performed on an Agilent HC-C8 column (4.6 mm x 250 mm, 5μm) with gradient elution. The mobile phase was acetonitrile-0.5% acetic acid. The injection volume was 6μL. The flow rate was 0.5 mL·min-1. The temperature of drift tube was set at 90℃. And the gas flow (N2) was set at 1.2 mL·min-1. The results showed that there were large differences in the quality ofRadix Platycodonis from different origins with the common fingerprints of 6 batches of samples fromChifeng in Inner Mongolia as references. The quality ofRadix Platycodonis was closely related to the seeds, the ecological environment, the way of drying and storing and so on. It was concluded that it was important to strengthen the provenance base construction, standardization of the seeds, reasonable formulation of the regionalization, and standardization of the production processing for the cultivation and production ofRadix Platycodonis.