ABSTRACT
RESUMOO presente artigo relata a elaboração de um modelo dinâmico de sistemas com o estudo de material reciclado proveniente de escombros para a fabricação de blocos de concreto do tipo não estruturais, utilizando para a modelagem das variáveis o programa computacional Stella. Essa modelagem permite que seja feita uma análise de cada variável do processo de produção, servindo como base para a definição das atividades críticas e as possibilidades de melhora e otimização do processo de produção, além de ser um critério de validação para uma adequada reutilização dos resíduos da construção, tendo em vista as legislações atuais e especificações técnicas e ambientais. Este estudo permitiu o desenvolvimento de um modelo conceitual de análise do processo de produção dos blocos através do comportamento que apresenta as variáveis que intervém no processo, demonstrando que a utilização de resíduos de construção e demolição neste estudo de caso é uma alternativa socioambiental com potencial econômico.
ABSTRACTThis work presents the development of a system dynamic model for the use of recycled material from construction and demolition waste in the production of non-structural concrete blocks, using the software Stella for modeling of parameters. The modeling allows the individual analysis of the production process parameters, serving as a base for the definition of critical activities and possibilities for the improvement and optimization of the production. The modeling is also a validation criterion to ensure an efficient reuse of construction waste, given the current regulations, environmental and technical specifications. This study resulted in a conceptual analysis model considering several variables related to the production process. It was possible to demonstrate that the use of construction and demolition waste is a feasible social and environmental activity with economic potential.
ABSTRACT
Cancer stem cells (CSCs) are often characterized by the elevated expression of drug-resistance related stem-cell surface markers, such as CD133 and ABCG2. Recently, we reported that CSCs have a high level of expression of the IL-6 receptor (IL-6R). The purpose of this study was to investigate the effect of anticancer drugs on the expression of the drug resistance-related cancer stem cell markers, ABCG2, IL-6R, and CD133 in non-small cell lung cancer (NSCLC) cell lines. A549, H460, and H23 NSCLC cell lines were treated with the anticancer drugs 5-fluorouracil (5-FU; 25 microg/ml) and methotrexate (MTX; 50 microg/ml), and the expression of putative CSC markers was analyzed by fluorescent activated cell sorter (FACS) and the gene expression level of abcg2, il-6r and cd133 by reverse transcriptasepolymerase chain reaction (RT-PCR). We found that the fraction of ABCG2-positive(+) cells was significantly increased by treatment with both 5-FU and MTX in NSCLC cells, and the elevation of abcg2, il-6r and cd133 expressions in response to these drugs was also confirmed using RT-PCR. Also, the number of IL-6R(+) cells was increased by MTX in the 3 cell lines mentioned and increased by 5-FU in the H460 cell line. The number of CD133(+) cells was also significantly increased by both 5-FU and MTX treatment in all of the cell lines tested. These results indicate that 5-FU and MTX considerably enhance the expression of drug-resistance related CSC markers in NSCLC cell lines. Thus, we suggest that antimetabolite cancer drugs, such as 5-FU and MTX, can lead to the propagation of CSCs through altering the expression of CSC markers.
Subject(s)
Carcinoma, Non-Small-Cell Lung , Cell Line , Drug Resistance , Fluorouracil , Gene Expression , Methotrexate , Neoplastic Stem Cells , Receptors, Interleukin-6ABSTRACT
Este trabalho objetivou apresentar os resultados de um diagnóstico anual da geração e da composição dos resíduos de construção e demolição de Fortaleza. Durante 12 meses, foram colhidas informações das empresas coletoras e da prefeitura, tais como a quantidade e o preço do metro cúbico do entulho coletado e o destino dado a ele. Foram ainda coletadas amostras de resíduos de construção e demolição nos locais de descarte licenciados pela prefeitura. Os resultados apontam que os locais licenciados recebem em média 702 toneladas.dia-1, sendo que a composição desses resíduos de construção e demolição é, em média, de 65 por cento de restos de argamassa, concreto e material cerâmico. Constatou-se também que grande parte desse tipo de resíduo é descartado ilegalmente, sendo que os dois principais aterros irregulares somam uma área aproximada de 26 hectares. Estima-se que esse sistema movimenta R$ 4,5 milhões anualmente e que quase metade desse montante é aportado somente pela Prefeitura Municipal.
This article aimed to present the results of an annual diagnosis of generation and composition of Fortalezaâs construction and demolition waste. During 12 months CDW collection companiesâ and the city hallâ information were researched, such as the amount and the price of the cubic meter of the collected wastes and the destiny given to them. Samples of construction and demolition waste were either collected at the licensed disposal places. The results point that licensed places receive 702 tons.day-1, on average, and the composition of this construction and demolition waste is made, on average, by 65 percent of mortar, concrete and ceramic material. It was also verified that great part of this waste is discarded illegally, and the two main irregular disposal sites have an approximate area of 26 hectares. It is estimated that system costs approximately R$4.5 million annually and almost half of this amount is paid only by the Municipal City hall.
ABSTRACT
The red cell deformability (RCD) has been taken as a diagnostic aid in recent years. The dilute red cell suspension passes through the silicon microch annels under constant suction pressure. Such three parameters of RCD are measure d as IF (filtration index), and The results show that the above-mentioned parameters can reflect the changes of cell rheological characteristics from dif ferent aspects. With the observation of the flow passage, the rehological charac teristics can be evaluated accurately and comprehensively.