Effects of RAB1A on the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells / 华西口腔医学杂志

OBJECTIVE@#This study aimed to investigate the molecular mechanism of RAB1A in the proliferation, invasion, and metastasis of human tongue squamous cell carcinoma.@*METHODS@#Western blot was used to detect the expression of RAB1A protein in human normal tongue epithelial cells (Hacat) and tongue squamous cell carcinoma Tca8113. The changes in RAB1A after plasmid transfection were also studied. The Tca8113 cells were named SiRAB1A/Tca8113 after RAB1A plasmid transfection. The expression of the epithelial-mesenchymal transition (EMT)-related markers of SiRAB1A/Tca8113 cells was also detected. CCK-8 assay was used to detect the proliferation of SiRAB1A/Tca8113 cells. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of SiRAB1A/Tca8113 cells, respectively.@*RESULTS@#Western blot results showed that the expression of RAB1A in tongue squamous cell carcinoma cells was significantly higher than that in Hacat. RAB1A decreased significantly after SiRAB1A plasmid transfection. CCK-8 proliferation assay showed that the proliferation of SiRAB1A/Tca8113 cells also decreased significantly. Transwell and wound healing assays demonstrated that the invasive and metastatic abilities of SiRAB1A/Tca8113 cells decreased significantly, respectively. In addition, Western blot results demonstrated that RAB1A deletion significantly increased the expression of E-cadherin and inhibited the expression of Vimentin.@*CONCLUSIONS@#RAB1A could promote the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells.

Research advances of Rab1A in the development of cancer / 医学研究生学报

Rab1A is a member of the RAB family as a small GTPase and a mammalian target of rapamycin complex 1 (mTORC1) Activator,which has been well established to mediate vesicular trafficking from the endoplasmic reticulum (ER) to the Golgi apparatus. With increasingly deep research of Rab1A,many researchers discover that Rab1A protein is also involved in mediating signal transduction,cell migration and regulation of autophagy. Meanwhile,aberrant expression of Rab1A has been linked to a range of human diseases as well,including Parkinson's disease,cardiomyopathy,and aspirin-exacerbated respiratory disease. Recently Rab1A research has gradually shifted to its role in occurrence and development of tumor,and researchers also discover that Rab1A was abnor-mally expressed in many malignant tumors such as tonguesquamous carcinoma,breast cancer,human lung cancer,hepatocellular carci-noma,colorectal cancer,gastric cancer and cervical cancer. Besides that,over expression of Rab1A plays a significant role in the pro-gression of different tumors. This article summarizes the research progress of Rab1A intumor development and signaling pathways.