ABSTRACT
OBJECTIVE: To analyze the growth arrest and DNA damage gene 45( GADD45) mRNA expression in peripheral blood of radiation workers,and its relationship with exposure dose. METHODS: Using a typical sampling method,97 radiation workers from a hospital of Guangdong were selected as radiation group,48 hospital administrative staff without exposure to radiation and other occupational hazard factors were selected as control group. The real-time quantitative polymerase chain reaction was used to detect GADD45 mRNA expression of two groups. With the use of thermoluminescence dosimetry,we detected and collected personal dose data of radiation group over the past year. RESULTS: The GADD45 mRNA relative expression of the radiation group was higher than that of the control group( P < 0. 01).There was no correlation between GADD45 mRNA relative expression and factors such as personal dose,sex,age,length of service,smoking,and alcohol drinking( P > 0. 05). CONCLUSION: The ionizing radiation can lead to up-regulation of GADD45 mRNA expression in peripheral blood of radiation workers,but there was no linear relationship between GADD45 mRNA expression and its personal dose.
ABSTRACT
OBJECTIVE: To investigate the occupational stress level of radiation exposed workers in Guangdong Province and explore the factors that influence occupational stress. METHODS: By random sampling method,306 radiation workers of Guangdong Province were selected in this study. The simplified Chinese version of Effort-Reward Imbalance( ERI)questionnaire was used to assess occupational stress levels. The scores of 3 dimensions including external effort,reward and internal commitment and their differences were analyzed. RESULTS: The scores of external effort,reward and internal commitment of 306 radiation workers in this study were( 15. 83 ± 5. 18),( 46. 63 ± 9. 06) and( 14. 97 ± 2. 23),respectively. There were 42 workers( 13. 73%) who had self-detected the occupational stress resulted from effort-reward imbalance; 62 workers( 20. 26%) were at high-risk of occupational stress. Compared with the industrial radiation workers,the scores of external effort and detection rate of occupational stress of hospital radiation workers were higher( P < 0. 05),while the high risk detection rate of occupational stress was lower( P < 0. 05). Compared with the female radiation workers, male workers had higher scores of external effort, effort / reward ratio and the detection rate of occupational stress( P < 0. 05) and lower scores of the reward score( P < 0. 05). The scores of external effort of radiation workers with junior college educational level or above were higher than those with senior high school educational level or below( P < 0. 05). CONCLUSION: The occupational stress level of radiation exposed workers has multiple influencing factors.It is recommended to strengthen the social support to improve their social and working environment,in order to reduce their occupational stress level.